Sensitivity and limitations of high throughput fluorescent microsatellite analysis for the detection of allelic imbalance: application in lung tumors.

2000 ◽  
Author(s):  
T Liloglou ◽  
P Maloney ◽  
G Xinarianos ◽  
S Fear ◽  
J K Field
Keyword(s):  
High Throughput ◽  
Allelic Imbalance ◽  
Lung Tumors ◽  
Microsatellite Analysis

Microsatellite analysis of allelic imbalance in tumour and blood from patients with prostate cancer

2008 ◽  
Vol 102 (2) ◽  
pp. 253-258 ◽  
Author(s):  
Heidi Schwarzenbach ◽  
Felix K.-H. Chun ◽  
Imke Müller ◽  
Christoph Seidel ◽  
Karoline Urban ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Allelic Imbalance ◽  
Microsatellite Analysis

scholarly journals High-throughput microsatellite analysis using fluorescent dUTPs for high-resolution genetic mapping of the mouse genome.

1997 ◽  
Vol 7 (1) ◽  
pp. 81-86 ◽  
Author(s):  
M Rhodes ◽  
A Dearlove ◽  
R Straw ◽  
S Fernando ◽  
A Evans ◽  
...  
Keyword(s):  
High Resolution ◽  
Genetic Mapping ◽  
High Throughput ◽  
Mouse Genome ◽  
Microsatellite Analysis

Modified Rapid DNA Extraction Protocol for High Throughput Microsatellite Analysis in Wheat

Crop Science ◽  
2002 ◽  
Vol 42 (6) ◽  
pp. 2088-2091 ◽  
Author(s):  
Kristi L. Hill-Ambroz ◽  
Gina L. Brown-Guedira ◽  
John P. Fellers
Keyword(s):  
Dna Extraction ◽  
High Throughput ◽  
Microsatellite Analysis ◽  
Extraction Protocol

Allelic Imbalance Demonstrated by Microsatellite Analysis of Lung Samples From Patients With Familial Pulmonary Fibrosis

CHEST Journal ◽  
2002 ◽  
Vol 121 (3) ◽  
pp. 25S ◽  
Author(s):  
Alan Q. Thomas ◽  
Jennifer Carneal ◽  
Cheryl Markin ◽  
Kirk B. Lane ◽  
John A. Phillips ◽  
...  
Keyword(s):  
Pulmonary Fibrosis ◽  
Allelic Imbalance ◽  
Microsatellite Analysis ◽  
Familial Pulmonary Fibrosis

Loss of Heterozygosity on Chromosomes 1, 2, 8, 9 and 17 in Cerebral Atherosclerotic plaques

2001 ◽  
Vol 16 (3) ◽  
pp. 167-171 ◽  
Author(s):  
P. Miniati ◽  
G. Sourvinos ◽  
M. Michalodimitrakis ◽  
D.A. Spandidos
Keyword(s):  
Loss Of Heterozygosity ◽  
Allelic Imbalance ◽  
Genetic Alterations ◽  
Microsatellite Analysis ◽  
Atherosclerotic Plaques ◽  
Potential Implication ◽  
Molecular Alterations ◽  
Significant Incidence ◽  
Polymerase Chain ◽  
Chromosomal Loci

Objective Atherosclerosis is a fibroproliferative disease which has been attributed to several factors including genetic and molecular alterations. Initial studies have shown genetic alterations at the microsatellite level in the DNA of atherosclerotic plaques. Extending our initial findings, we performed a microsatellite analysis on cerebral atherosclerotic plaques. Methods Twenty-seven cerebral atherosclerotic plaques were assessed for loss of heterozygosity (LOH) and microsatellite instability (MI) using 25 microsatellite markers located on chromosomes 2, 8, 9 and 17. DNA was extracted from the vessels as well as the respective blood from each patient and subjected to polymerase chain reaction. Results Our analyses revealed that specific loci on chromosomes 2, 8, 9 and 17 exhibited a significant incidence of LOH. Forty-six percent of the specimens showed loss of heterozygosity at 2p13–p21, 48% exhibited LOH at 8p12–q11.2, while allelic imbalance was detected in 47% of the cases. The LOH incidence was 39%, 31% and 27% at 17q21, 9q31–34 and 17p13, respectively. Genetic alterations were detected at a higher rate as compared to the corresponding alterations observed in plaques from other vessels. Discussion This is the first microsatellite analysis using atherosclerotic plaques obtained from cerebral vessels. Our results indicate an elevated mutational rate on specific chromosomal loci, suggesting a potential implication of these regions in atherogenesis.

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