scholarly journals Establishment and characterization of gastric carcinoma cell clones expressing LMP2A of Epstein-Barr virus

2009 ◽  
Vol 25 (01) ◽  
Author(s):  
Lee
Keyword(s):  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Carcinoma Cell ◽  
Gastric Carcinoma Cell ◽  
Barr Virus ◽  
Cell Clones ◽  
Epstein Barr

The full-length DNA sequence of Epstein Barr virus from a human gastric carcinoma cell line, SNU-719

Virus Genes ◽  
2015 ◽  
Vol 51 (3) ◽  
pp. 329-337 ◽  
Author(s):  
Kyung-A Song ◽  
San-Duk Yang ◽  
Jinha Hwang ◽  
Jong-Il Kim ◽  
Myung-Soo Kang
Keyword(s):  
Gastric Carcinoma ◽  
Cell Line ◽  
Dna Sequence ◽  
Epstein Barr Virus ◽  
Carcinoma Cell ◽  
Gastric Carcinoma Cell ◽  
Gastric Carcinoma Cell Line ◽  
Human Gastric Carcinoma ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein-Barr Virus Promotes Epithelial Cell Growth in the Absence of EBNA2 and LMP1 Expression

1999 ◽  
Vol 73 (2) ◽  
pp. 1286-1292 ◽  
Author(s):  
Jun Nishikawa ◽  
Shosuke Imai ◽  
Takanori Oda ◽  
Toshichika Kojima ◽  
Kiwamu Okita ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Nuclear Antigen ◽  
Population Doubling ◽  
Term Survival ◽  
Barr Virus ◽  
Ebv Infection ◽  
Cell Clones ◽  
Lmp1 Expression ◽  
Epstein Barr

ABSTRACT We attempted to infect primary gastric epithelia (PGE) with recombinant Epstein-Barr virus (EBV) carrying a selectable marker that made it possible to select EBV-infected cells. Cells dually positive for EBV-determined nuclear antigen (EBNA) and cytokeratin were detected in 3 of 21 primary cultures after 3 days of EBV inoculation. From one culture, EBV-infected cell clones were repeatedly obtained at a frequency of 3 to 5 cell clones per 106 cells. EBV-infected clones had enhanced population doubling and grew to attain a highly increased saturation density, together with acquisition of marked anchorage independence. The infected clones retained the ultrastructural morphology characteristic of gastric mucosal epithelium and have been growing stably for more than 18 months (corresponding to at least 300 generations) so far, in clear contrast to the parental PGE cells, which ceased growth after 60 generations. The p53 gene of the parental PGE cells was found to be overexpressed, perhaps thereby conferring the basal potential for long-term survival in vitro. Moreover, EBV infection accelerated, to a significant extent, the growth rate and agar clonability of NU-GC-3 cells, an established EBV-negative but EBV-susceptible human gastric carcinoma cell line. Both EBV-converted PGE and NU-GC-3 clones, like EBV-positive gastric carcinoma biopsy specimens, expressed a restricted set of EBV latent infection genes characterized by the absence of EBNA2 and latent membrane protein 1 (LMP1) expression. These results indicate that EBV infection causes a transformed phenotype on PGE in the setting of possible unregulated cell cycling and renders even established gastric carcinoma cells more malignant via a limited spectrum of viral latent-gene expression. This study may reflect an in vivo scenario illustrating multiphasic involvement of EBV in carcinogenesis of gastric or other epithelial cancers.

Characterization of Epstein-Barr virus gp350/220 gene variants in virus isolates from gastric carcinoma and nasopharyngeal carcinoma

2011 ◽  
Vol 157 (2) ◽  
pp. 207-216 ◽  
Author(s):  
Bing Luo ◽  
Mengyang Liu ◽  
Yan Chao ◽  
Yun Wang ◽  
Yongzheng Jing ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Gene Variants ◽  
Barr Virus ◽  
Epstein Barr ◽  
Virus Isolates

scholarly journals Expression of Viral MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma

2006 ◽  
Vol 81 (2) ◽  
pp. 1033-1036 ◽  
Author(s):  
Do Nyun Kim ◽  
Hiun-Suk Chae ◽  
Sang Taek Oh ◽  
Jin-Hyoung Kang ◽  
Cho Hyun Park ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Carcinoma Cells ◽  
Gastric Carcinoma Cell ◽  
Barr Virus ◽  
Carcinoma Cell Lines ◽  
Tumor Tissues ◽  
Gastric Carcinoma Cell Lines ◽  
Epstein Barr ◽  
Bart Mirnas

ABSTRACT Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs). We found that BART miRNAs but not BHRF1 miRNAs were expressed in EBV-infected gastric carcinoma cell lines and the tumor tissues from patients as well as the animal model. The expression of viral miRNAs in EBVaGCs suggests that these EBV miRNAs may play important roles in the tumorigenesis of EBVaGCs.

Establishment and characterization of an in vivo model for Epstein–Barr virus positive gastric carcinoma

2007 ◽  
Vol 79 (9) ◽  
pp. 1343-1348 ◽  
Author(s):  
Sang Taek Oh ◽  
Jung-Ho Cha ◽  
Dong-Jik Shin ◽  
Sungjoo Kim Yoon ◽  
Suk Kyeong Lee
Keyword(s):  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
In Vivo Model ◽  
Barr Virus ◽  
Epstein Barr
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