scholarly journals Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

2020 ◽  
Author(s):  
Ping Liu ◽  
Jeong‑Wook Choi ◽  
Min‑Kyeong Lee ◽  
Youn Choi ◽  
Taek‑Jeong Nam
Keyword(s):  
Mouse Model ◽  
Wound Repair ◽  
Skin Wound ◽  
Full Thickness ◽  
Excisional Wound

scholarly journals The role of placenta-derived mesenchymal stem cells in healing of induced full-thickness skin wound in a mouse model

IUBMB Life ◽  
2015 ◽  
Vol 67 (9) ◽  
pp. 701-709 ◽  
Author(s):  
Somia H. Abd-Allah ◽  
Amal S. El-Shal ◽  
Sally M. Shalaby ◽  
Eman Abd-Elbary ◽  
Nehad F. Mazen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

Evaluation of the Effect of the Structure of Bacterial Cellulose on Full Thickness Skin Wound Repair on a Microfluidic Chip

2015 ◽  
Vol 16 (3) ◽  
pp. 780-789 ◽  
Author(s):  
Ying Li ◽  
Shiwen Wang ◽  
Rong Huang ◽  
Zhuo Huang ◽  
Binfeng Hu ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Microfluidic Chip ◽  
Wound Repair ◽  
Skin Wound ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals A New Role for Activin in Endometrial Repair after Menses

Endocrinology ◽  
2008 ◽  
Vol 150 (4) ◽  
pp. 1904-1911 ◽  
Author(s):  
Tu'uhevaha J. Kaitu'u-Lino ◽  
David J. Phillips ◽  
Naomi B. Morison ◽  
Lois A. Salamonsen
Keyword(s):  
Mouse Model ◽  
Wound Repair ◽  
Abnormal Uterine Bleeding ◽  
Transgenic Animals ◽  
Skin Wound ◽  
Uterine Bleeding ◽  
Epithelial Cell Line ◽  
Skin Wound Healing ◽  
Endometrial Epithelial Cell

Abnormal uterine bleeding can severely affect the quality of life for women. After menstruation, the endometrium must adequately repair to limit and stop bleeding. Abnormal uterine bleeding may result from incorrect or inadequate endometrial repair after menstruation. Previous studies have shown an important contribution of activin to skin wound healing, with severely delayed wound repair observed in animals transgenically induced to overexpress activin’s natural inhibitor, follistatin. Activin subunits have also been identified within human endometrium; however, their role in endometrial repair is unknown. We assessed the contribution of activin to endometrial repair after menses using a human in vitro cell wounding method and our well-characterized mouse model of endometrial breakdown and repair applied to mice overexpressing follistatin. Endometrial repair after menses is initiated with reepithelialization of the uterine surface. To mimic this repair, we utilized a human endometrial epithelial cell line (ECC-1) and demonstrated significant stimulation of wound closure after activin A administration, and attenuation of this response by addition of follistatin. Immunolocalization of activin subunits, βA and βB, in control endometrium from the mouse model demonstrated specific epithelial and stromal localization and some leukocyte staining (βA) around sites of endometrial repair, suggestive of a role for activin in this process. Follistatin-overexpressing animals had significantly higher circulating follistatin levels than wild-type littermates. There was a significant delay in endometrial repair after breakdown in follistatin transgenic animals compared with control animals. This study demonstrates for the first time a functional role for activin in endometrial repair after menses.

scholarly journals Injectable Self-Healing Ceria-based Nanocomposite Hydrogel with ROS-scavenging Activity for Skin Wound Repair

2021 ◽  
Author(s):  
Xueyun Gong ◽  
Meng Luo ◽  
Min Wang ◽  
Wen Niu ◽  
Yidan Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Skin Wound ◽  
Scavenging Activity ◽  
Self Healing ◽  
Nanocomposite Hydrogel ◽  
Full Thickness ◽  
Ros Scavenging ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract Excessive reactive oxygen species (ROS) in the injured skin may impede the wound repair and skin regeneration. Herein, we develop an injectable self-healing ceria-based nanocomposite hydrogel with ROS-scavenging activity to accelerate wound healing. The nanocomposite hydrogels were successfully prepared by coating cerium oxide nanorods with polyethylenimine (PEI) and crosslinked with benzaldehyde-terminated F127 (F127-CHO) through the dynamic Schiff-base reaction (FVEC hydrogel). The results showed that the FVEC hydrogel possessed the good thermosensitivity, injectability, self-healing ability and ROS scavenging activity. The subcutaneous implantation experiments in mice confirmed that FVEC hydrogels are biocompatible and biodegradable in vivo. The full-thickness skin wound studies showed that FVEC hydrogel could significantly enhance the wound healing and epithelium regeneration with the formation of hair follicle and adipocyte tissue. This work provides a new strategy for the development of multifunctional Ce-based nanocomposite hydrogel for full-thickness skin wound healing and regeneration.

scholarly journals Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Poonam Shakya ◽  
A. K. Sharma ◽  
Naveen Kumar ◽  
Remya Vellachi ◽  
Dayamon D. Mathew ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Repair ◽  
Healing Process ◽  
Skin Wound ◽  
Skin Wounds ◽  
Full Thickness ◽  
Open Wound ◽  
Full Thickness Skin ◽  
Group I ◽  
Thickness Skin

An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P<0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

scholarly journals A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model

2020 ◽  
Vol 21 (12) ◽  
pp. 4508 ◽  
Author(s):  
Ilia Banakh ◽  
Perdita Cheshire ◽  
Mostafizur Rahman ◽  
Irena Carmichael ◽  
Premlatha Jagadeesan ◽  
...  
Keyword(s):  
Mouse Model ◽  
Wound Repair ◽  
Human Fibroblasts ◽  
Skin Wound ◽  
Tissue Growth ◽  
Skin Substitute ◽  
Protein Levels ◽  
Collagen Iii ◽  
Synthetic Grafts ◽  
Dermal Template

Engineered dermal templates have revolutionised the repair and reconstruction of skin defects. Their interaction with the wound microenvironment and linked molecular mediators of wound repair is still not clear. This study investigated the wound bed and acellular “off the shelf” dermal template interaction in a mouse model. Full-thickness wounds in nude mice were grafted with allogenic skin, and either collagen-based or fully synthetic dermal templates. Changes in the wound bed showed significantly higher vascularisation and fibroblast infiltration in synthetic grafts when compared to collagen-based grafts (P ≤ 0.05). Greater tissue growth was associated with higher prostaglandin-endoperoxide synthase 2 (Ptgs2) RNA and cyclooxygenase-2 (COX-2) protein levels in fully synthetic grafts. Collagen-based grafts had higher levels of collagen III and matrix metallopeptidase 2. To compare the capacity to form a double layer skin substitute, both templates were seeded with human fibroblasts and keratinocytes (so-called human skin equivalent or HSE). Mice were grafted with HSEs to test permanent wound closure with no further treatment required. We found the synthetic dermal template to have a significantly greater capacity to support human epidermal cells. In conclusion, the synthetic template showed advantages over the collagen-based template in a short-term mouse model of wound repair.

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