scholarly journals Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27

2016 ◽  
Vol 39 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Cho-Rong Kim ◽  
Young-Min Kim ◽  
Min-Kyeong Lee ◽  
In-Hye Kim ◽  
Youn-Hee Choi ◽  
...  
Keyword(s):  
Cell Line ◽  
Signaling Pathway ◽  
Collagen Synthesis ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Pyropia Yezoensis ◽  
Fibroblast Cell Line ◽  
Smad Signaling ◽  
Smad Signaling Pathway

scholarly journals Fermentation of Panax notoginseng root extract polysaccharides attenuates oxidative stress and promotes type I procollagen synthesis in human dermal fibroblast cells

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shiquan You ◽  
Xiuqin Shi ◽  
Dan Yu ◽  
Dan Zhao ◽  
Quan An ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Signaling Pathway ◽  
Dermal Fibroblast ◽  
Panax Notoginseng ◽  
Human Dermal Fibroblast ◽  
Down Regulation ◽  
Smad Signaling ◽  
Smad Signaling Pathway

Abstract Background Panax notoginseng is one of the most valuable traditional Chinese medicines. Polysaccharides in P. notoginseng has been shown to significantly reduce the incidence of human diseases. However the application of fermentation technology in Panax notoginseng is not common, and the mechanism of action of P. notoginseng polysaccharides produced by fermentation is still unclear. The specific biological mechanisms of fermented P. notoginseng polysaccharides (FPNP) suppresses H2O2-induced apoptosis in human dermal fibroblast (HDF) and the underlying mechanism are not well understood. Methods In this study, the effects of water extracted and fermentation on concentration of polysaccharides in P. notoginseng extracts were analyzed. After the H2O2-induced HDF model of oxidative damage was established, and then discussed by the expression of cell markers, including ROS, MDA, SOD, CAT, GSH-Px and MMP-1, COL-I, ELN, which were detected by related ELISA kits. The expression of TGF-β/Smad pathway markers were tested by qRT-PCR to determine whether FPNP exerted antioxidant activity through TGF-β signaling in HDF cells. Results The polysaccharide content of Panax notoginseng increased after Saccharomyces cerevisiae CGMCC 17452 fermentation. In the FPNP treatment group, ROS and MDA contents were decreased, reversed the down-regulation of the antioxidant activity and expression of antioxidant enzyme (CAT, GSH-Px and SOD) induced by H2O2. Furthermore, the up-regulation in expression of TGF-β, Smad2/3 and the down-regulation in the expression of Smad7 in FPNP treated groups revealed that FPNP can inhibit H2O2-induced collagen and elastin injury by activating TGF-β/Smad signaling pathway. Conclusion It was shown that FPNP could inhibit the damage of collagen and elastin induced by H2O2 by activating the TGF-β/Smad signaling pathway, thereby protecting against the oxidative damage induced by hydrogen peroxide. FPNP may be an effective attenuating healing agent that protects the skin from oxidative stress and wrinkles.

Morphologic, Immunophenotypic and Bioenergetic Characterization of a Human Dermal Fibroblast Cell Line

2014 ◽  
Author(s):  
Ioana Zinuca Pavel ◽  
Oana Duicu ◽  
Florina Bojin ◽  
Andreea Privistirescu ◽  
Dorina Coricovac ◽  
...  
Keyword(s):  
Cell Line ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cell Line

scholarly journals Morphologic, Immunophenotypic and Bioenergetic Characterization of a Human Dermal Fibroblast Cell Line

2014 ◽  
Author(s):  
Ioana Zinuca Pavel ◽  
Oana Duicu ◽  
Florina Bojin ◽  
Andreea Privistirescu ◽  
Dorina E Coricovac ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Line ◽  
Mitochondrial Function ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Seeding Density ◽  
Glycolytic Flux ◽  
Fibroblast Cell Line

Background In the past years, ultraviolet-B (UVB)-induced morphological changes in cultured fibroblasts have been systematically studied in relation with photoprotective strategies. The present study was aimed at providing a comprehensive characterization of a human dermal fibroblast cell line, HDFa, in terms of: (i) morphological and phenotypical aspects, and (ii) mitochondrial function and bioenergetics. Methods Morphology of HDFa line was assessed by light microscopy and flow cytometry was used for the evaluation of the the following surface markers: CD34, CD45, CD90, CD73, CD44, CD29, CD117, CD105, CD109, CD26, IL-10R, HLA-DR and TGF-βRIII. Cell monolayers were fixed and stained for CD105, CD117, Ki67, Vimentin, α-smooth muscle actin, Caldesmon, Cytokeratin, and smooth muscle myosin by immunofluorescence. Mitochondrial bioenergetics was measured by using Seahorse Bioscience XF24 extracellular flux analyzer. Different number of cells were seeded in order to obtain the optimal seeding density needed to identify a measurable OCR (oxygen consumption rate) and ECAR (extracellular acidification rate). The following protocol for mitochondrial function assay was tested at 37°C: Basal respiration followed by sequential injections of Oligomycin, FCCP, and Antimycin A (BOFA). Parameters of mitochondrial respiratory function (basal OCR, maximal respiratory capacity, ATP production, and proton leak) together with those of glycolysis (glycolytic flux, glycolytic capacity, and glycolytic reserve), respectively were measured. After each BOFA assay, cells were lysed to quantify total protein by Bradford assay. Results HDFa cells showed elongated flattened form, were adherent and rapidly expanded in culture. HDFa cells were positive for CD90, CD73, CD26, and CD29 at flowcytometry, whereas imunofluorescence microscopy revealed that the cell line was positive for CD105, Ki67, and Vimentin. The optimal mitochondrial and glycolytic flux parameters were reported at 20.000 cells/well, resulting that this seeding density will be used in our further experiments. Conclusions Characterization of HDFa cell line was performed in order to further report on their changes in response to different protocols of UVB exposure in the presence vs. the absence of protective phytochemicals. Research supported by the doctoral fellowship POSDRU/159 /1.5/S/ 136893.

Evaluation of a near-senescent human dermal fibroblast cell line and effect of amelogenin

2009 ◽  
Vol 160 (6) ◽  
pp. 1163-1171 ◽  
Author(s):  
S. Almqvist ◽  
M. Werthén ◽  
A. Johansson ◽  
J. Törnqvist ◽  
M.S. Ågren ◽  
...  
Keyword(s):  
Cell Line ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cell Line
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