scholarly journals Xuebijing attenuates hypotension through the upregulation of angiotensin II type 1 receptor-associated protein 1 in rats suffering from heat stroke

2014 ◽  
Vol 34 (6) ◽  
pp. 1699-1705 ◽  
Author(s):  
ZHIGUO PAN ◽  
YU SHAO ◽  
WENPENG DONG ◽  
CHENXI LIU ◽  
YI CHEN ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Heat Stroke ◽  
Receptor Associated Protein

Involvement of Runx3 in the basal transcriptional activation of the mouse angiotensin II type 1 receptor-associated protein gene

2011 ◽  
Vol 43 (14) ◽  
pp. 884-894 ◽  
Author(s):  
Miyuki Matsuda ◽  
Kouichi Tamura ◽  
Hiromichi Wakui ◽  
Toru Dejima ◽  
Akinobu Maeda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Angiotensin Ii ◽  
Transcriptional Activation ◽  
At1 Receptor ◽  
Distal Convoluted Tubule ◽  
Luciferase Assay ◽  
Serum Starvation ◽  
Receptor Associated Protein ◽  
Tubule Cells

We previously cloned a molecule that interacts with angiotensin II type 1 (AT1) receptor to exert an inhibitory function on AT1 receptor signaling that we named ATRAP/ Agtrap (for AT1 receptor-associated protein). In the present study we examined the regulation of basal ATRAP gene expression using renal distal convoluted tubule cells. We found that serum starvation upregulated basal expression of ATRAP gene, a response that required de novo mRNA and protein synthesis. Luciferase assay revealed that the proximal promoter region directs transcription and that a putative binding site of runt-related transcription factors (RBE) is important for transcriptional activation. The results of RBE-decoy transfection and endogenous knockdown by small interference RNA showed that the runt-related transcription factor Runx3 is involved in ATRAP gene expression. Chromatin immunoprecipitation assay also supported the binding of Runx3 to the ATRAP promoter in renal distal convoluted tubule cells. Immunohistochemistry demonstrated the expression of Runx3 and ATRAP proteins in the distal convoluted and connecting tubules of the kidney in consecutive sections. Furthermore, the Runx3 immunostaining was decreased together with a concomitant suppression of ATRAP expression in the affected kidney after 7 days of unilateral ureteral obstruction. These findings indicate that Runx3 plays a role in ATRAP gene expression in renal distal tubular cells both in vitro and in vivo.

scholarly journals Deletion of the angiotensin II type 1 receptor–associated protein enhances renal sodium reabsorption and exacerbates angiotensin II–mediated hypertension

2014 ◽  
Vol 86 (3) ◽  
pp. 570-581 ◽  
Author(s):  
Masato Ohsawa ◽  
Kouichi Tamura ◽  
Hiromichi Wakui ◽  
Akinobu Maeda ◽  
Toru Dejima ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Sodium Reabsorption ◽  
Receptor Associated Protein ◽  
Renal Sodium Reabsorption

Association of angiotensin II type 1 receptor-associated protein gene polymorphism with increased mortality in septic shock*

2011 ◽  
Vol 39 (7) ◽  
pp. 1641-1648 ◽  
Author(s):  
Taka-aki Nakada ◽  
James A. Russell ◽  
John H. Boyd ◽  
Luke McLaughlin ◽  
Emiri Nakada ◽  
...  
Keyword(s):  
Septic Shock ◽  
Angiotensin Ii ◽  
Gene Polymorphism ◽  
Protein Gene ◽  
Receptor Associated Protein
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