scholarly journals Naringenin attenuates the release of pro-inflammatory mediators from lipopolysaccharide-stimulated BV2 microglia by inactivating nuclear factor-κB and inhibiting mitogen-activated protein kinases

2012 ◽  
Author(s):  
Yung Hyun Choi
Keyword(s):  
Protein Kinases ◽  
Inflammatory Mediators ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein ◽  
Bv2 Microglia

scholarly journals Bacteroides fragilis Enterotoxin Induces Intestinal Epithelial Cell Secretion of Interleukin-8 through Mitogen-Activated Protein Kinases and a Tyrosine Kinase-Regulated Nuclear Factor-κB Pathway

2004 ◽  
Vol 72 (10) ◽  
pp. 5832-5839 ◽  
Author(s):  
Shaoguang Wu ◽  
Jan Powell ◽  
Nes Mathioudakis ◽  
Sheryl Kane ◽  
Ellen Fernandez ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Protein Kinases ◽  
Bacteroides Fragilis ◽  
Nuclear Factor Κb ◽  
Interleukin 8 ◽  
Biologically Active ◽  
Nuclear Factor ◽  
Intestinal Epithelial ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

ABSTRACT Enterotoxigenic Bacteroides fragilis (ETBF) secretes a 20-kDa metalloprotease toxin termed B. fragilis toxin (BFT). ETBF disease in animals is associated with an acute inflammatory response in the intestinal mucosa, and lethal hemorrhagic colitis may occur in rabbits. In this study, we confirmed recent reports (J. M. Kim, Y. K. Oh, Y. J. Kim, H. B. Oh, and Y. J. Cho, Clin. Exp. Immunol. 123:421-427, 2001; L. Sanfilippo, C. K. Li, R. Seth, T. J. Balwin, M. J. Menozzi, and Y. R. Mahida, Clin. Exp. Immunol. 119:456-463, 2000) that purified BFT stimulates interleukin-8 (IL-8) secretion by human intestinal epithelial cells (HT29/C1 cells) and demonstrate that stimulation of IL-8 production is dependent on biologically active BFT and independent of serum. Induction of IL-8 mRNA expression occurs rapidly and ceases by 6 h after BFT treatment, whereas IL-8 secretion continues to increase for at least 18 h. Our data suggest that BFT-stimulated IL-8 secretion involves tyrosine kinase-dependent activation of nuclear factor-κB (NF-κB) as well as activation of the mitogen-activated protein kinases (MAPKs), p38 and extracellular signal-related kinase. Simultaneous activation of NF-κB and MAPKs appears necessary for secretion of IL-8 by HT29/C1 cells treated with BFT.

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