scholarly journals Therapeutic effects of nerve leachate‑treated adipose‑derived mesenchymal stem cells on rat sciatic nerve injury

2019 ◽  
Author(s):  
Yumei Liu ◽  
Ruiqi Dong ◽  
Chunyan Zhang ◽  
Yuxiang Yang ◽  
Yaolu Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Therapeutic Effects ◽  
Rat Sciatic Nerve

Repairing sciatic nerve injury with an EPO-loaded nerve conduit and sandwiched-in strategy of transplanting mesenchymal stem cells

Biomaterials ◽  
2017 ◽  
Vol 142 ◽  
pp. 90-100 ◽  
Author(s):  
Wei Zhang ◽  
Lihai Zhang ◽  
Jianheng Liu ◽  
Licheng Zhang ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Nerve Conduit

Differentiated Mesenchymal Stem Cells for Sciatic Nerve Injury

2011 ◽  
Vol 7 (3) ◽  
pp. 664-671 ◽  
Author(s):  
Michal Dadon-Nachum ◽  
Ofer Sadan ◽  
Itay Srugo ◽  
Eldad Melamed ◽  
Daniel Offen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury

scholarly journals Schwann Cell-Like Cells Derived from Human Amniotic Mesenchymal Stem Cells Promote Peripheral Nerve Regeneration through a MicroRNA-214/c-Jun Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Wei Chen ◽  
Shune Xiao ◽  
Zairong Wei ◽  
Chengliang Deng ◽  
Kaiyu Nie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Molecular Mechanisms ◽  
Peripheral Nerve Regeneration ◽  
Sciatic Nerve Injury ◽  
Amniotic Mesenchymal Stem Cells

Background. The use of Schwann cell-like cells (SCLCs) derived from stem cells has been introduced as an effective strategy for promoting peripheral nerve regeneration (PNR). However, molecular mechanisms underlying therapeutic transplantation of SCLCs for PNR are often ignored. Objectives. To explore the potential of SCLCs for the treatment of sciatic never injury and investigate the underlying molecule mechanisms. Method. SCLCs differentiated from human amniotic mesenchymal stem cells (hAMSCs) and specific markers of Schwann cells were detected. SCLCs were transplanted into the injured sites of a rat model of sciatic nerve injury, and sciatic nerve functional index (SFI) was determined. Results. SCLCs expressed specific markers of Schwann cells as well as secreted neurotrophic factors. The transplantation of SCLCs into injured sites of a rat model of sciatic nerve injury promoted the functional recovery. With regard to the underlying molecular mechanisms, we identified c-Jun as a negative regulator of the myelination of SCLCs. Moreover, we discovered a novel signaling transduction pathway in SCLCs; that is, miR-214 directly targets c-Jun to promote the myelination of SCLCs. Finally, we demonstrated that miR-214 upon overexpression in SCLCs enhanced the therapeutic effects of SCLCs on sciatic nerve injury. Conclusions. We demonstrate that SCLCs have beneficial effect for myelination. Moreover, our results provide a previously unknown molecular basis underlying the treatment of peripheral nerve injury with SCLCs and also offer a practical strategy for future therapeutic promotion of PNR.

Effect of Schwann and mesenchymal stem cells on experimentally induced sciatic nerve injury in rats

2015 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
RedaA AboElsoud ◽  
FawzyA Ashour ◽  
AmaniA Elbaz ◽  
FadiaM Attia ◽  
SafaaM El-Kotb
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Experimentally Induced

scholarly journals Effects of Adipose-Derived Mesenchymal Stem Cells and Human Amniotic Membrane on Sciatic Nerve Repair in Rats

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Siyawash Xaki ◽  
Afshin Fathi ◽  
Mehdi Ariana ◽  
Hamid Reza Aghayan ◽  
Babak Arjmand ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Amniotic Membrane ◽  
Nerve Fibers ◽  
Sciatic Nerve Injury ◽  
Control Group ◽  
Human Amniotic Membrane

Background: Peripheral nerve injuries remain a great challenge for microsurgery despite the significant progress in recent decades. The current gold standard is autogenous nerve grafting with a success rate as low as 50% in long gaps. Current studies have focused on finding alternative methods for bridging nerve defects. Previous data have demonstrated the role of human amniotic membrane in stimulating neural regeneration. On the other hand, adipose-derived mesenchymal stem cells can differentiate into all three germ layers and could support nerve repair. The purpose of this study was to compare the role of the human amniotic membrane with and without adipose tissue stem cells in sciatic nerve injury with gap in rats. Objectives: We aimed to evaluate the effectiveness of the human amniotic membrane with and without adipose-derived mesenchymal stem cells in sciatic nerve injury with gap in rats. Methods: Twenty-four male Wistar rats in four random groups were used in our study. In the first group, the nerve gap was repaired using the inverse resected nerve segment (Control group), the second group was repaired with a human amniotic membrane (AM group), the third group was repaired with an amnion sheet with seeded adipose-derived mesenchymal stem cells (AM/ADMSCs group), and the last group was not repaired, and both stumps were sutured to muscles. Results: All the animals underwent the procedures and survived without complication. The sciatic function index and hot plate test results were significantly improved in the AM and AM/ADMSCs groups compared to the Control group (as a gold standard of care) (P>0.05). Based on histopathology findings, regenerative nerve fibers were seen in the implanted area of both AM and AM/ADMSCs groups; however, nerve fibers were surrounded by significant fibrosis (scar formation) in the AM/ADMSCs group. The axon count in the Control group was significantly higher than both experimental groups (P < 0.01). Conclusions: Our study showed the role of amniotic membrane in the promotion of nerve regeneration in sciatic nerve injury with a gap, but adding adipose-derived mesenchymal stem cells not only has no extra benefits, but also causes more tissue scar.

Sign in / Sign up

Export Citation Format

Share Document