scholarly journals Yes‑associated protein (YAP) predicts poor prognosis and regulates progression of esophageal squamous cell cancer through epithelial‑mesenchymal transition

2019 ◽  
Author(s):  
Yan Qu ◽  
Lin Zhang ◽  
Jianbo Wang ◽  
Pengxiang Chen ◽  
Yibin Jia ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Poor Prognosis ◽  
Epithelial Mesenchymal Transition ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Esophageal Squamous Cell Cancer ◽  
Mesenchymal Transition

547 THE ROLE OF HEAT SHOCK PROTEIN 90 IN HYPOXIA INDUCED ANGIOGENESIS AND EPITHELIAL-MESENCHYMAL TRANSITION OF ESOPHAGEAL SQUAMOUS CELL CANCER

2021 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Ching Tzao ◽  
Chin-Kun Wang
Keyword(s):  
Cell Migration ◽  
Heat Shock ◽  
Squamous Cell ◽  
Epithelial Mesenchymal Transition ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Esophageal Squamous Cell Cancer ◽  
Mesenchymal Transition ◽  
And Migration

Abstract   Hypoxia is known as an important trigger for the development of metastases in human cancers. Heat shock proteins (Hsps) are up-regulated by cellular stressors including hypoxia. To date, the functional role of Hsps within the hypoxic tumor microenvironment for esophageal squamous cell cancer (ESCC) remains poorly defined. Methods CoCl₂ was used to induce hypoxia in cultured ESCC cells which was confirmed by 2′,7′ –dichlorofluorescin diacetate (DCFDA) assay. 7-Dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG), a selective Hsp90 inhibitor, was used to treat 2 ESCC cell lines, KYSE-170 and -510 cells pretreated with or without CoCl₂₂₂₂₂ in different concentrations, followed by cytotoxicity (MTT) and migration assays. In parallel, expression of Hsp90, vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1α (HIF-1α), and markers related to epithelial-mesenchymal transition (EMT) such as snail/E-cadherin, by immunoblot or ELISA while analyzing cell proliferation and migration of treated ESCC cells. Results CoCl₂ induced hypoxia was supported by induction of reactive oxygen species (ROS). CoCl₂ (200 μM) significantly suppressed cell viability and proliferation with a concomitant up-regulation of VEGF and HIF-1α in a dose-dependent fashion. In contrast, cell migration was significantly increased in response to CoCl₂ while down-regulating E-cadherin with a concomitant increase in Snail expression. 17-DMAG decreased expression of VEGF and HIF-1α while inhibiting cell migration and invasion. Conclusion Our data demonstrate that CoCl2 induced hypoxia promotes EMT and angiogensis, which are inhibited by 17-DMAG. These results suggest that hypoxia induced EMT and angiogensis is Hsp90 dependent in ESCC.

scholarly journals PTTG1 cooperated with GLI1 leads to epithelial-mesenchymal transition in esophageal squamous cell cancer

Oncotarget ◽  
2017 ◽  
Vol 8 (54) ◽  
pp. 92388-92400 ◽  
Author(s):  
Wang Feng ◽  
Xuan Xiaoyan ◽  
Li Shenglei ◽  
Liu Hongtao ◽  
Jiang Guozhong
Keyword(s):  
Squamous Cell ◽  
Epithelial Mesenchymal Transition ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Esophageal Squamous Cell Cancer ◽  
Mesenchymal Transition

scholarly journals Low expression of BEX1 predicts poor prognosis in patients with esophageal squamous cell cancer

2018 ◽  
Author(s):  
Hai‑Tao Geng ◽  
Zhi‑Wen Cheng ◽  
Rui‑Juan Cao ◽  
Zhen‑Bo Wang ◽  
Shao‑Zhi Xing ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Poor Prognosis ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Esophageal Squamous Cell Cancer ◽  
Low Expression

scholarly journals STAT3 regulates hypoxia-induced epithelial mesenchymal transition in oesophageal squamous cell cancer

2016 ◽  
Vol 36 (1) ◽  
pp. 108-116 ◽  
Author(s):  
YAO CUI ◽  
YUN-YUN LI ◽  
JIAN LI ◽  
HONG-YAN ZHANG ◽  
FENG WANG ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Epithelial Mesenchymal Transition ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Mesenchymal Transition
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