scholarly journals BMP7 coordinates endometrial epithelial cell receptivity for blastocyst implantation through the endoglin pathway in cell lines and a mouse model

2019 ◽  
Author(s):  
Caixia Yuan ◽  
Xianlian Li ◽  
Haixia Song ◽  
Lingling Fan ◽  
Shili Su ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mouse Model ◽  
Cell Lines ◽  
Endometrial Epithelial Cell ◽  
Blastocyst Implantation

Expression and function of Toll-like receptors in human endometrial epithelial cell lines

2010 ◽  
Vol 84 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Wedad Aboussahoud ◽  
Reza Aflatoonian ◽  
Chris Bruce ◽  
Sarah Elliott ◽  
Jon Ward ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Toll Like Receptors ◽  
Endometrial Epithelial Cell ◽  
Epithelial Cell Lines ◽  
And Function

Differential expression of 17beta-hydroxysteroid dehydrogenases types 2 and 4 in human endometrial epithelial cell lines

2000 ◽  
Vol 24 (1) ◽  
pp. 135-144 ◽  
Author(s):  
B Husen ◽  
N Psonka ◽  
M Jacob-Meisel ◽  
C Keil ◽  
GM Rune
Keyword(s):  
Epithelial Cell ◽  
Mrna Expression ◽  
Differential Expression ◽  
Cell Lines ◽  
Calf Serum ◽  
Human Endometrium ◽  
Rt Pcr ◽  
Serum Replacement ◽  
Endometrial Epithelial Cell ◽  
Epithelial Cell Lines

In the endometrium two enzymes are known to convert estradiol to its inactive metabolite estrone: microsomal 17beta-hydroxysteroid dehydrogenase type 2 (17beta-HSD2) and peroxisomal 17beta-HSD4. In order to elucidate the particular function of each of these two different enzymes, the human endometrial epithelial cell lines HEC-1-A and RL95-2 were examined with respect to the expression of 17betaHSD isozymes. They were compared with human endometrium in vivo. Non-radioactive in situ hybridization revealed both enzymes in glandular epithelial cells of human endometrium. The two cell lines were screened for mRNA expression of 17beta-HSD 1-4 by RT-PCR and Northern blot. 17beta-HSD2 and 4 could be detected by either method, 17beta-HSD1 only by RT-PCR, 17beta-HSD3 not at all. Both cell lines were proven to have no receptor for progesterone which is known as a physiological inducer of several 17beta-HSD isozymes. To study the regulation of 17beta-HSD2 and 17betaHSD4, the concentration of fetal calf serum in the cell culture media was reduced stepwise to 0.3% by dilution with a defined serum replacement. This treatment led to an inhibition of 17beta-HSD2 mRNA expression and an increase in the mRNA expression of 17beta-HSD4. Concomitantly, distinct morphological changes were observed, such as a decrease in the number and length of microvilli and a decrease in the formation of domes on top of the monolayers. The endometrial epithelial cell lines HEC-1-A and RL95-2 represent a suitable in vitro model for further studies of the differential expression of the major endometrial HSD isozymes, independent of the effect of progesterone.

Cell surfactomes of two endometrial epithelial cell lines that differ in their adhesiveness to embryonic cells

2014 ◽  
Vol 81 (4) ◽  
pp. 326-340 ◽  
Author(s):  
Sonali R. Bhagwat ◽  
Tejashree Redij ◽  
Kruttika Phalnikar ◽  
Sumeet Nayak ◽  
Swati Iyer ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Embryonic Cells ◽  
Endometrial Epithelial Cell ◽  
Epithelial Cell Lines

scholarly journals The effects of sex steroid hormones and interleukin-1-beta on MUC1 expression in endometrial epithelial cell lines

Reproduction ◽  
2006 ◽  
Vol 131 (4) ◽  
pp. 733-742 ◽  
Author(s):  
A W Horne ◽  
E-N Lalani ◽  
R A Margara ◽  
J O White
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Interleukin 1 ◽  
Flow Cytometric Analysis ◽  
Endometrial Receptivity ◽  
Muc1 Expression ◽  
Interleukin 1 Beta ◽  
Similar Treatment ◽  
Endometrial Epithelial Cell ◽  
Epithelial Cell Lines

Oestrogen, progesterone and paracrine signals from the embryo have been associated with the overall control of implantation. Changes in the expression of the heavily glycosylated transmembrane glycoprotein MUC1 mucin on the endometrial epithelium are also thought to be important for embryo attachment. Increased MUC1 expression has been correlated with elevated progesterone levels in the secretory phase of the menstrual cycle. Embryonic control of endometrial receptivity through changes in MUC1 expression could be achieved through the interleukin-1 system. Four endometrial epithelial cell lines (HEC1A, HEC1B, Ishikawa and RL592) were treated with oestrogen and progesterone (with or without interleukin-1-beta) and were subjected to immunocytochemistry and flow cytometric analysis to determine MUC1 production using MUC1 antibodies. HEC1A (oestrogen receptor (ER) and progesterone receptor (PR) positive) and HEC1B (ER positive and PR negative) were transfected with theMUC1promoter, underwent similar treatment regimes and the activity of theMUC1promoter relative to their untreated controls was determined using a chloramphenicol acetyltransferase (CAT) enzyme-linked immunoassay. Using the cell lines, we determined that endometrial MUC1 expression is up-regulated by progesterone, consistent with thein vivoincreases in MUC1 related to high progesterone levels. We also revealed that neither oestrogen, nor interleukin-1-beta, appear to modulate MUC1. Progesterone-dependent regulation of MUC1 is likely to be an important factor in determining endometrial receptivity.

Human endometrial epithelial cell lines for studying steroid and cytokine actions

1990 ◽  
Vol 26 (12) ◽  
pp. 1173-1179 ◽  
Author(s):  
S. Tabibzadeh ◽  
K. L. Kaffka ◽  
P. L. Kilian ◽  
P. G. Satyaswaroop
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Endometrial Epithelial Cell ◽  
Epithelial Cell Lines

scholarly journals Pseudomonas aeruginosa Gene Products PilT and PilU Are Required for Cytotoxicity In Vitro and Virulence in a Mouse Model of Acute Pneumonia

1999 ◽  
Vol 67 (7) ◽  
pp. 3625-3630 ◽  
Author(s):  
James C. Comolli ◽  
Alan R. Hauser ◽  
Leslie Waite ◽  
Cynthia B. Whitchurch ◽  
John S. Mattick ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cell ◽  
Mouse Model ◽  
Cell Lines ◽  
Twitching Motility ◽  
Wild Type ◽  
Epithelial Cell Lines ◽  
Acute Pneumonia

ABSTRACT Type IV pili of the opportunistic pathogen Pseudomonas aeruginosa mediate twitching motility and act as receptors for bacteriophage infection. They are also important bacterial adhesins, and nonpiliated mutants of P. aeruginosa have been shown to cause less epithelial cell damage in vitro and have decreased virulence in animal models. This finding raises the question as to whether the reduction in cytotoxicity and virulence of nonpiliated P. aeruginosa mutants are primarily due to defects in cell adhesion or loss of twitching motility, or both. This work describes the role of PilT and PilU, putative nucleotide-binding proteins involved in pili function, in mediating epithelial cell injury in vitro and virulence in vivo. Mutants of pilT and pilU retain surface pili but have lost twitching motility. In three different epithelial cell lines, pilT or pilU mutants of the strain PAK caused less cytotoxicity than the wild-type strain but more than isogenic, nonpiliated pilA or rpoN mutants. ThepilT and pilU mutants also showed reduced association with these same epithelial cell lines compared both to the wild type, and surprisingly, to a pilA mutant. In a mouse model of acute pneumonia, the pilT and pilUmutants showed decreased colonization of the liver but not of the lung relative to the parental strain, though they exhibited no change in the ability to cause mortality. These results demonstrate that pilus function mediated by PilT and PilU is required for in vitro adherence and cytotoxicity toward epithelial cells and is important in virulence in vivo.

scholarly journals Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

2015 ◽  
Vol 20 (3) ◽  
pp. 399-409 ◽  
Author(s):  
Xiangguo Wang ◽  
Pengfei Lin ◽  
Yanlong Yin ◽  
Jinhua Zhou ◽  
Lanjie Lei ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Er Stress ◽  
Cell Lines ◽  
Vaccine Strain ◽  
Endometrial Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Brucella Suis
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