scholarly journals Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells

2018 ◽  
Author(s):  
Chunhui Wang ◽  
Huimei Zang ◽  
Dongsheng Zhou
Keyword(s):  
Femoral Head ◽  
Bone Morphogenetic Protein ◽  
Bone Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Therapeutic Benefits ◽  
Morphogenetic Protein

Bone morphogenetic protein 2 controls steroid-induced osteonecrosis of the femoral head via directly inhibiting interleukin-34 expression

2021 ◽  
Author(s):  
Meng Wang ◽  
Hong Sung Min ◽  
Haojie Shan ◽  
Yiwei Lin ◽  
Wenyang Xia ◽  
...  
Keyword(s):  
Femoral Head ◽  
Bone Morphogenetic Protein ◽  
Inflammatory Responses ◽  
Gene Knockout ◽  
Osteoclast Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Qrt Pcr ◽  
Morphogenetic Protein ◽  
Local Expression

Increased inflammatory responses is one of the major characteristics of osteonecrosis of the femoral head (ONFH). We aimed to investigate the function of bone morphogenetic protein 2 (BMP-2)/interleukin (IL)-34 axis in the inflammatory responses of ONFH. The systemic and local expression of BMPs in ONFH patients were detected by qRT-PCR and ELISA. In vitro osteoclast differentiation and ONFH mouse models, induced by 20 mg/kg methylprednisolone through intramuscular injection, were established using wild type and BMP-2-/- mice to explore the regulatory role of BMP-2 in pro-inflammatory responses and bone defects of ONFH. IL-34 expression and function were examined in vitro and in vivo through qRT-PCR, TRAP staining, and gene knockout. The systemic and local expression of BMPs were elevated in ONFH patients. BMP-2 reduced the production of pro-inflammatory cytokines and inhibited the differentiation of osteoclasts. Mechanistically, BMP-2 inhibited osteoclasts formation through suppressing IL-34 expression, and then promoted bone repair and alleviated ONFH. In conclusion, our study reveals that BMP-2 inhibits inflammatory responses and osteoclast formation through down-regulating IL-34.

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