scholarly journals Inhibitory effect of burdock leaves on elastase and tyrosinase activity

2017 ◽  
Vol 14 (4) ◽  
pp. 3247-3252 ◽  
Author(s):  
Chi-Ting Horng ◽  
Hsing-Chen Wu ◽  
Ni-Na Chiang ◽  
Chiu-Fang Lee ◽  
Yu-Syuan Huang ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Tyrosinase Activity

scholarly journals Inhibitory Effect of Gnetin C, a Resveratrol Dimer from Melinjo (Gnetum gnemon), on Tyrosinase Activity and Melanin Biosynthesis

2012 ◽  
Vol 35 (6) ◽  
pp. 993-996 ◽  
Author(s):  
Miyako Yanagihara ◽  
Maiko Yoshimatsu ◽  
Akinori Inoue ◽  
Tomoko Kanno ◽  
Tomoki Tatefuji ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Melanin Biosynthesis ◽  
Gnetum Gnemon

scholarly journals Anti-Melanogenic Effects of Hydroxyectoine via MITF Inhibition by JNK, p38, and AKT Pathways in B16F10 Melanoma Cells

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1985852 ◽  
Author(s):  
You C. Chung ◽  
Min-Jin Kim ◽  
Eun Y. Kang ◽  
Yun B. Kim ◽  
Bong S. Kim ◽  
...  
Keyword(s):  
Skin Color ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Dependent Manner ◽  
Related Protein ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
B16f10 Cells ◽  
Dose Dependent ◽  
Tyrosinase Related Protein

Melanin plays a role in determining human skin color of a person, and a large amount of melanin makes the skin color look darkened. The proper amount of melanin formation protects our skin from UV radiation, but excessive melanin production causes hyperpigmentation and leads to freckles, melasma, and lentigo. In this study, we investigated the inhibitory effect of hydroxyectoine on melanogenesis and its mechanism in B16F10 cells. Melanin content and cellular tyrosinase activity were determined. The expression of microphthalmia-associated transcription factor (MITF), and the activities of tyrosinase and other melanogenesis-related enzymes, such as tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2, were also examined. Hydroxyectoine treatment significantly inhibited melanin production and intracellular tyrosinase activity in a dose-dependent manner. Western blot analysis showed that hydroxyectoine also reduced the expressions of tyrosinase and TRP-1. In addition, hydroxyectoine significantly reduced the expression of MITF, a major regulator of melanin production, and inhibited the phosphorylation of p38, c-Jun N-terminal kinase, and activated the protein kinase B. The results demonstrated that hydroxyectoine inhibits the expression of MITF through the inhibition or activation of melanin-related signaling pathways and downregulates melanogenesis by inhibiting melanogenic enzyme expression and tyrosinase activity. Hydroxyectoine has potential value in functional cosmetics applications, such as whitening.

scholarly journals Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Haixia Lu ◽  
Ke Yang ◽  
Lianghui Zhan ◽  
Tingting Lu ◽  
Xue Chen ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Dendrobium Officinale ◽  
Key Factors ◽  
Extraction Technology ◽  
Steepest Ascent ◽  
Burman Design ◽  
Solid Liquid

In order to establish the extraction technology of flavonoids from Dendrobium officinale leaves, a method combining Plackett–Burman design (PBD), steepest ascent design, and central composite design was developed to optimize the extraction of flavonoids. In addition, the tyrosinase activity inhibition of flavonoids was further tested in vitro. PBD results showed that ethanol concentration and number of extractions were key factors. Response surface methodology (RSM) indicated that the optimal extraction conditions were 78% ethanol concentration, six extraction times, 2 h, and 1:50 solid-liquid ratio. Under these conditions, the total flavonoid content could reach 35 mg/50 mL. In vitro tyrosinase experiment, the extracted total flavonoids had better inhibitory effect on tyrosinase activity than β-arbutin, and its inhibition rate for monophenolase and diphenolase exceeded 100% and 70%, respectively. These results indicate that RSM can effectively improve the extraction of flavonoids from Dendrobium officinale leaves and the flavonoids have the prospect of being applied to foods and cosmetics.

Inhibition of Tyrosinase by Protocatechuic Aldehyde

2004 ◽  
Vol 32 (01) ◽  
pp. 97-103 ◽  
Author(s):  
Jae Kyung No ◽  
Min Sun Kim ◽  
You Jung Kim ◽  
Song Ja Bae ◽  
Jae Sue Choi ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Salvia Miltiorrhiza ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Medical Problems ◽  
Melanin Biosynthesis ◽  
Rate Limiting Step ◽  
Protocatechuic Aldehyde ◽  
Dependent Inhibition ◽  
Potent Inhibitory Effect

The purpose of this study was to determine the inhibitory action of protocatechuic aldehyde (PCA) on tyrosinase activity. PCA is one of the compounds found in the root of Salvia miltiorrhiza. Our study documented that PCA has a potent inhibitory effect on tyrosinase, which catalyzes the rate-limiting step of melanin biosynthesis. Although melanin biosynthesis has an essential function normally in human skin for defense against ultraviolet light of the sun, its abnormal activity as seen in pigmentation disorder could lead to serious medical problems. Our data showed that PCA, with concentrations ranging from 1×10-5 M to 8×10-5 M , exhibited dose-dependent inhibition of the enzyme activity with 50% of inhibition at 19.92×10-6 M . A further kinetic analysis on PCA inactivation of tyrosinase activity revealed a competitive inhibition of the enzyme at the L-tyrosine binding site. The findings of our present study merit further research on the applicability of PCA as a potential agent for treatment of pigmentation disorder.

scholarly journals Tyrosinase Inhibitory Effect, Antioxidant and Anticancer Activities of Bioactive Compounds in Ripe Hog Plum (Spondias Pinnata) Fruit Extracts

2019 ◽  
Vol 35 (3) ◽  
pp. 916-926
Author(s):  
Supawadee Patathananone ◽  
Jureerut Daduang ◽  
Amonrat Koraneekij ◽  
Chia-Ying Li
Keyword(s):  
High Performance ◽  
Antioxidant Property ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Normal Fibroblast ◽  
Anticancer Activities ◽  
Ic50 Value ◽  
Bioactive Agents ◽  
Hog Plum

The usage of ripe hog plum fruit (Spondias pinnata) extracts in cosmetics and food products, including cancer therapeutic agents, have a few studies. Herein, the strong anti-tyrosinase activity found in the extracted part of isopropanol is reported. This extract was separated by liquid/liquid extraction using hexane: methanol+H2O. The hydrophilic layer (6A*) exhibited the anti-tyrosinase, antioxidant, and anticancer activities in vitro. The IC50 value of each bioactivity was presented as approximately 0.18, 0.04, and 1.40 mg/ml, respectively. In addition, 6A* fraction showed a very low cytotoxic effect in normal fibroblast cells (NHDF cells). The bioactive agents in 6A* were purified by C18 reverse-phase High-Performance Liquid Column Chromatography (HPLC). The 12 purified peaks were shown in the chromatogram profile. All peaks (excepted 6A-06 and 6A-09) displayed anti-tyrosinase activity, whereas the antioxidant property was not found in 6A-01, 6A-06, and 6A-08 but was represented in other peaks. Most purified peaks were indicated to be the aromatic alcohol or derivative phenol compounds.

scholarly journals Camellia japonica Essential Oil Inhibits α-MSH-Induced Melanin Production and Tyrosinase Activity in B16F10 Melanoma Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Si Young Ha ◽  
Ji Young Jung ◽  
Jae-Kyung Yang
Keyword(s):  
Essential Oil ◽  
Inhibitory Activity ◽  
Treated Group ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Tyrosinase Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Camellia Japonica ◽  
Tyrosinase Inhibitory Activity ◽  
Natural Substances

Essential oils are aromatic oils extracted from the leaves, stems, peels, petals, and roots of aromatic plants grown in nature or grown in organic methods and have various medical effects as natural substances. The essential oil extracted from Camellia japonica seeds exhibits various functional properties; however, its tyrosinase inhibitory activity has not been investigated extensively. This study is performed to investigate the chemical composition and tyrosinase inhibitory activity of Camellia japonica seed essential oil (CJS-EO). Hexamethylcyclotrisiloxane (42.36%) and octamethylcyclotetrasiloxane (23.28%) are the two primary components of CJS-EO, as identified via gas chromatography-mass spectrometry. The inhibitory activities of CJS-EO and positive control arbutin are further evaluated against mushroom tyrosinase. The results show that CJS-EO and arbutin inhibit tyrosinase activity. Moreover, CJS-EO significantly inhibits melanogenesis in the α-melanocyte-stimulating hormone-treated group, and a significant amount of melanin is suppressed. To ascertain the cause of the CJS-EO tyrosinase inhibitory effect and melanin reduction effect, genetic and protein analyses are performed. Based on our results, we tentatively conclude that CJS-EO can inhibit melanocytes from harmful factors such as tyrosinase-related protein. These results demonstrate that CJS-EO possesses potent antityrosinase activity and may be a good skin-whitening agent.

Inhibitors of Tyrosinase

1955 ◽  
Vol 32 (3) ◽  
pp. 468-484
Author(s):  
M. G. M. PRYOR
Keyword(s):  
Methylene Blue ◽  
Dissolved Oxygen ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Aromatic Substrate ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Low Concentrations ◽  
Sulphydryl Groups

1. It has been reported that if Drosophila larvae are ground to a fine paste with sand, the homogenate shows little tyrosinase activity, but that if the larvae are allowed to blacken in chloroform vapour before grinding, activity is increased. 2. This has been interpreted as showing the effect of an intracellular inhibitor, set free by rupturing the cells, but destroyed by chloroform. This inhibitor has been identified by previous authors as a dehydrogenase. 3. It is here suggested that the lack of activity of Drosophila extracts prepared with sand is due to destruction of tyrosinase as it oxidizes naturally occurring aromatic substrates. 4. It is shown that tyrosinase is destroyed by oxidizing the aromatic substrate present in the cuticle of Calliphora larvae, or by very low concentrations of homocatechol. 5. The aromatic substrate of Calliphora larvae is concentrated in the cuticle, and would be set free by fine grinding. 6. Drosophila or Calliphora larvae yield a more active extract when ground with sand than when simply crushed, provided that they are tested soon after grinding. 7. The tyrosinase activity of such extracts is not increased by chloroform or methanol. 8. The compound between o-quinones and amino-acids is capable of oxidizing ascorbic acid or excess amino-acid without the aid of an enzyme, and of simultaneously reducing methylene blue. 9. This reaction, rather than the activity of dehydrogenases, is probably responsible for most of the ability of damaged insect tissue to bleach methylene blue. 10. The blood of insects normally contains dissolved oxygen in equilibrium with the air. 11. The reaction involved in the blackening of insect blood may consume all the dissolved oxygen. 12. Previous observations on fluctuations in the oxidation-reduction potential of the blood of Calliphora larvae with age are probably due to changes in the rate at which oxygen is consumed by the blood after it is shed. 13. There does not therefore appear to be any valid evidence that tyrosinase is inhibited in vivo by the action of dehydrogenases. The absence of tyrosinase activity in undamaged tissue is probably due to the structure of the cytoplasm, which keeps enzyme and substrate apart. 14. Instances of the inhibition of tyrosinase reported in Crustacea and Echinodermata seem to be susceptible of the same explanation as in insects. 15. The supposed inhibitory effect of sulphydryl groups reported for vertebrate melanophores is shown to be due to the combination of sulphydryl groups with o-quinones, which prevents the formation of melanins.

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