scholarly journals Electroacupuncture attenuates learning and memory impairment via activation of α7nAChR-mediated anti-inflammatory activity in focal cerebral ischemia/reperfusion injured rats

2017 ◽  
Vol 14 (2) ◽  
pp. 939-946 ◽  
Author(s):  
Jiao Liu ◽  
Chunyan Li ◽  
Hongwei Peng ◽  
Kunqiang Yu ◽  
Jing Tao ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Learning And Memory ◽  
Memory Impairment ◽  
Focal Cerebral Ischemia ◽  
Ischemia Reperfusion ◽  
Inflammatory Activity ◽  
Cerebral Ischemia Reperfusion ◽  
Anti Inflammatory

N-Nitro-L-Arginine-Methyl ester Exerts Neuroprotection By Inhibiting Inflammation In Cerebral Injury Induced By Transient Ischemia/Reperfusion In Rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Awooda Hiba A
Keyword(s):  
Nitric Oxide ◽  
Cerebral Ischemia ◽  
Methyl Ester ◽  
Focal Cerebral Ischemia ◽  
Ischemia Reperfusion ◽  
Cerebral Injury ◽  
Cerebral Ischemia Reperfusion ◽  
Transient Focal Cerebral Ischemia ◽  
Tnf Α ◽  
Anti Inflammatory

Cerebral ischemia-reperfusion injury is a pivotal cause of deaths due to cerebrovascular accidents. Further research efforts are needed to reveal the mechanism underlying its aggravation or alleviation. We previously reported the antioxidant effect of N-Nitro-L-Arginine-Methyl Ester (L-NAME), a nonselective nitric oxide synthase (NOS) inhibitor, on rats subjected to transient focal cerebral ischemia-reperfusion (I/R). The aim of this work was to explore further neuroprotective anti-inflammatory effects of L-NAME. This study involved 30 adult male Wistar rats divided into three groups with ten rats in each: sham-operated (control), I/R group of rats subjected to 30 minutes of left common carotid artery (CCA) occlusion followed by 24-hour of reperfusion and test group infused with L-NAME intraperitoneally 15 minutes before the same I/R period. Neurological assessments were evaluated, Western blotting used to estimate Nuclear factor-kappa B (NF-қB), ELISA used to detect Tumor necrosis factor- α (TNF-α), and Nitric oxide metabolites were measured colorimetrically, as well as H&E staining to assess brain damage. Compared with the I/R group, the neurological score, infarction area, and the inflammatory biomarkers NF-қB, TNF-α, and NO were significantly decreased in L-NAME treated rats (P ≤0.001). As a conclusion from the current study, L-NAME showed potential neuroprotection through it is an anti-inflammatory effect on a rat’s model of transient focal cerebral ischemia-reperfusion.

Sign in / Sign up

Export Citation Format

Share Document