scholarly journals Changes of adrenomedullin and natriuretic peptides in patients with adrenal medullary hyperplasia prior to and following pharmacological therapy and adrenalectomy

2016 ◽  
Vol 12 (2) ◽  
pp. 864-872 ◽  
Author(s):  
Pang-Hu Zhou ◽  
Lei Shi ◽  
Wei Hu ◽  
Xiao-Bin Zhang ◽  
Wei Wang ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Pharmacological Therapy ◽  
Adrenal Medullary Hyperplasia

Detection of atrial natriuretic peptides (ANP) in rat atria by immunogold-silver staining (IGSS)

1994 ◽  
Vol 52 ◽  
pp. 304-305
Author(s):  
Robyn Rufner ◽  
Gerhard W. Hacker ◽  
Michele Forte ◽  
Nancyleigh E. Carson ◽  
Cristina Xenachis ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Silver Staining ◽  
Sodium Thiosulfate ◽  
Atrial Natriuretic Peptides ◽  
Paraffin Sections ◽  
Glass Slides ◽  
Metallic Salts ◽  
Wistar Kyoto ◽  
Right Atria

The use of immunogold-silver staining (IGSS) to enhance label penetration and Localization for immunocytochemistry or in situ hybridization utilizing a variety of metallic salts has been documented. In this morphological study, the effects of silver acetate, silver lactate and silver nitrate were evaluated for immunogold-labeling of a trial natriuretic peptides (ANP) in rat right atria.Eight Wistar Kyoto retired breeders were sedated with pentobarbital, perfused with either 4% paraformaldehyde (LM) or Karnovsky's fixative (EM), and right atria were dissected, processed, embedded in paraffin or epon, respectively and sectioned according to conventional methods. For light microscopy, an indirect IGSS method according to Hacker (3) was performed. Paraffin sections on glass slides were washed in ddH2O, immersed in Lugol's iodine, washed in ddH2O and treated with 2.5% aqueous sodium thiosulfate for 20 sec. After additional washes in ddH2O and TBS-0.1% fish gelatin, 10% normal goat serum (PBS with 1% BSA) was applied for 20 min before an overnight incubation at 4°C with a polyclonal α-ANP primary antibody (Peninsula Labs, 1:1000 in TBS/BSA).

PENURUNAN SUHU TUBUH MENGGUNAKAN TEPID WATER SPONGING DENGAN PENDEKATAN KONSERVASI LEVINE

2019 ◽  
Vol 11 (1) ◽  
pp. 33-40
Author(s):  
Muhammad Khabib Burhanuddin Iqomh ◽  
Nani Nurhaeni ◽  
Dessie Wanda
Keyword(s):  
Body Temperature ◽  
Nursing Care ◽  
Human Body ◽  
Model Theory ◽  
Pharmacological Treatment ◽  
Scientific Work ◽  
Pharmacological Therapy ◽  
Nursing Process ◽  
Tepid Water ◽  
Conservation Model

Peningkatan suhu tubuh  menyebabkan rasa tidak nyaman, gelisah pada anak, sehingga waktu untuk istirahat menjadi terganggu.Tatalaksana pada anak dengan demam dapat dilakukan dengan metode farmakologi dan non farmakologi. Tepid water spongingmerupakan tatalaksana non farmakologi. Konservasi adalah serangkaian sistem agar tubuh manusia mampu menjalankan fungsi, beradaptasi untuk melangsungkan kehidupan. Perawat mempunyai peran untuk membantu anak dalam mengatasi gangguan termoregulasi. Karya ilmiah ini bertujuan untuk mengetahui efektifitas penurunan suhu tubuh menggunakan tepid water sponging dengan pendekatanl konservasi Levine di ruang rawat infeksi. Efektifitas diukur dalam pemberian asuhan keperawatan berdasarkan proses keperawatan yang terdapat dalam model konservasi Levine yaitu: pengkajian, menentukan trophicognosis, menentukan hipotesis, intervensi dan evaluasi. Terdapat lima kasus yang dibahas. Hasil penerapan model konservasi Levine mampu meningkatkan kemampuan anak dalam mempertahankan fungsi tubuh dan beradaptasi terhadap perubahan. Kombinasi tepid water sponging dan terapi farmakologi mampu mengatasi demam dengan cepat dibanding terapi farmakologi.   Kata kunci: termoregulasi, tepid water sponging, teori model konservasi Levine   REDUCTION OF BODY TEMPERATURE USING TEPID WATER SPONGINGWITH THE LEVINE CONSERVATION APPROACH   ABSTRACT Increased body temperature causes discomfort, anxiety in children, so that the time to rest becomes disturbed. Management of children with fever can be done by pharmacological and non-pharmacological methods. Tepid water sponging is a non-pharmacological treatment. Conservation is a series of systems so that the human body is able to function, adapt to life. Nurses have a role to help children overcome thermoregulation disorders. This scientific work aims to determine the effectiveness of decreasing body temperature using tepid water sponging with the approach of Levine conservation in the infectious care room. Effectiveness is measured in the provision of nursing care based on the nursing process contained in the Levine conservation model, namely: assessment, determining trophicognosis, determining hypotheses, intervention and evaluation. There are five cases discussed. The results of the application of the Levine conservation model are able to improve the ability of children to maintain body functions and adapt to changes. The combination of tepid water sponging and pharmacological therapy is able to overcome fever quickly compared to pharmacological therapy.   Keywords: thermoregulation, tepid water sponging, Levine conservation model theory  

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