scholarly journals Electric pulse current stimulation increases electrophysiological properties of If current reconstructed in mHCN4-transfected canine mesenchymal stem cells

2016 ◽  
Vol 11 (4) ◽  
pp. 1323-1329 ◽  
Author(s):  
YUANYUAN FENG ◽  
SHOUMING LUO ◽  
PAN YANG ◽  
ZHIYUAN SONG
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Pulse Current ◽  
Electric Pulse ◽  
Electrophysiological Properties ◽  
If Current

Electric-Pulse Current Stimulation Increases If Current in mShox2 Genetically Modified Canine Mesenchymal Stem Cells

Cardiology ◽  
2015 ◽  
Vol 132 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Yuanyuan Feng ◽  
Shouming Luo ◽  
Shifei Tong ◽  
Li Zhong ◽  
Changhai Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluorescent Protein ◽  
Pulse Current ◽  
Morphological Changes ◽  
Electric Pulse ◽  
Genetically Modified ◽  
Inward Current ◽  
Cardiomyogenic Differentiation ◽  
If Current

Objective: We aimed to investigate the role of mShox2 in generating If pacemaker current in vitro by means of electric-pulse current stimulation (EPCS) of canine mesenchymal stem cells (cMSCs). Methods: mShox2 genetically modified cMSCs were prepared with pLentis-mShox2 red fluorescent protein. After EPCS induction, we examined the kinetic characteristics of generated inward current by means of a patch clamp. We then evaluated the expression of pacemaker-related genes, such as Nkx2.5, Tbx3, HCN4, Cx43 and Cx45, by means of qRT-PCR and Western blotting. The morphological changes and the cardiomyogenic differentiation marker cTnT were investigated at the same time. Results: The time- and voltage-dependent inward current recorded after mShox2 infection was confirmed to be If current. After EPCS induction, the detection rate of this If current was increased. The current amplitude and density were increased, and the channel activation curve shifted to the right. The pacemaker markers Tbx3, HCN4 and Cx45 were significantly upregulated, but the working myocardium markers Nkx2.5 and Cx43 were downregulated after mShox2 infection, and were more remarkable after EPCS induction. The cells became larger and assumed spindle and spider-like morphologies. cTnT was also detected in the experimental cells. Conclusions: Our results suggest that EPCS promotes the differentiation of mShox2 genetically modified cMSCs into pacemaker-like cells, which generates more If current.

GATA-4 induces changes in electrophysiological properties of rat mesenchymal stem cells

2014 ◽  
Vol 1840 (6) ◽  
pp. 2060-2069 ◽  
Author(s):  
Hong-Xia Li ◽  
Ya-Feng Zhou ◽  
Bin Jiang ◽  
Xin Zhao ◽  
Ting-Bo Jiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrophysiological Properties

scholarly journals 5-Azacytidine induces changes in electrophysiological properties of human mesenchymal stem cells

Cell Research ◽  
2006 ◽  
Vol 16 (12) ◽  
pp. 949-960 ◽  
Author(s):  
Bartosz Balana ◽  
Cecilia Nicoletti ◽  
Ihor Zahanich ◽  
Eva M Graf ◽  
Torsten Christ ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Electrophysiological Properties

scholarly journals Electrophysiological properties of human mesenchymal stem cells

2004 ◽  
Vol 554 (3) ◽  
pp. 659-672 ◽  
Author(s):  
Jürgen F. Heubach ◽  
Eva M. Graf ◽  
Judith Leutheuser ◽  
Manja Bock ◽  
Bartosz Balana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Electrophysiological Properties

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

1417: Mesenchymal Stem Cells Alone or Modified with ENOS Restore Erectile Function in the Aged Rat: Cell-Based Gene Therapy for Erectile Dysfunction

2004 ◽  
Vol 171 (4S) ◽  
pp. 373-373
Author(s):  
Trinity J. Bivalacqua ◽  
Mustafa F. Usta ◽  
Hunter C. Champion ◽  
Weiwen Deng ◽  
Philip J. Kadowitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Mesenchymal Stem Cells ◽  
Erectile Dysfunction ◽  
Erectile Function ◽  
Aged Rat
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