scholarly journals Inhibitory effect of bone morphogenetic protein-2 on the proliferation of giant cell tumor of bone stromal cells in vitro

2015 ◽  
Vol 11 (1) ◽  
pp. 309-314
Author(s):  
BAOHUA HE ◽  
GUANPING HE ◽  
XIAOFEI ZHENG ◽  
LIHUA LI ◽  
MEI LI ◽  
...  
Keyword(s):  
Giant Cell Tumor ◽  
Giant Cell ◽  
Bone Morphogenetic Protein ◽  
Stromal Cells ◽  
Inhibitory Effect ◽  
Cell Tumor ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

scholarly journals The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Shalini Singh ◽  
Isabella W. Y. Mak ◽  
Divya Handa ◽  
Michelle Ghert
Keyword(s):  
Cell Migration ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Stromal Cells ◽  
Epithelial Mesenchymal Transition ◽  
Cell Tumor ◽  
Stable Cell Line ◽  
Mesenchymal Transition

Giant cell tumor of bone (GCT) is a bone tumor consisting of numerous multinucleated osteoclastic giant cells involved in bone resorption and neoplastic osteoblast-like stromal cells responsible for tumor growth. The tumor occasionally metastasizes to the lung; however, factors leading to metastasis in this tumor are unknown. The TWIST-1 protein (also referred to as TWIST) has been suggested to be involved in epithelial-mesenchymal transition (EMT) and tumor progression in some cancers. In this study we investigated the functional role of TWIST in GCT cell angiogenesis and migration. Overexpression of TWIST in neoplastic GCT stromal cells significantly increased mRNA and protein expression of VEGF and VEGFR1 in vitro, whereas knockdown of TWIST resulted in decreased VEGF and VEGFR1 expression. A stable cell line with TWIST overexpression resulted in features of EMT including increased cell migration and downregulation of E-cadherin. The results of our study indicate that TWIST may play an important role in angiogenesis and cell migration in GCT.

scholarly journals New Target for Precision Medicine Treatment of Giant-Cell Tumor of Bone: Sunitinib Is Effective in the Treatment of Neoplastic Stromal Cells with Activated PDGFRβ Signaling

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3543
Author(s):  
Michal Mahdal ◽  
Jakub Neradil ◽  
Peter Mudry ◽  
Silvia Paukovcekova ◽  
Iva Staniczkova Zambo ◽  
...  
Keyword(s):  
Growth Factor ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Stromal Cells ◽  
Growth Factor Receptor ◽  
Metastatic Potential ◽  
Giant Cells ◽  
Cell Tumor ◽  
Primary Bone Tumor

Giant-cell tumor of bone (GCTB) is an intermediate type of primary bone tumor characterized by locally aggressive growth with metastatic potential. The aim of this study was to identify new druggable targets among the cell signaling molecules involved in GCTB tumorigenesis. Profiles of activated signaling proteins in fresh-frozen tumor samples and tumor-derived cell lines were determined using phosphoprotein arrays. Analysis of the obtained data revealed epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor beta (PDGFRβ) as potential targets, but only the PDGFR inhibitor sunitinib caused a considerable decrease in stromal cell viability in vitro. Furthermore, in the case of a 17-year-old patient suffering from GCTB, we showed that the addition of sunitinib to the standard treatment of GCTB with the monoclonal antibody denosumab resulted in the complete depletion of multinucleated giant cells and mononuclear stromal cells in the tumor tissue. To summarize, the obtained data showed that a specific receptor tyrosine kinase (RTK) signaling pattern is activated in GCTB cells and plays an important role in the regulation of cell proliferation. Thus, activated RTKs and their downstream signaling pathways represent useful targets for precision treatment with low-molecular-weight inhibitors or with other types of modern biological therapy.

Influence of Surface Chemistry on the Biological Feature of Giant Cell Tumor of Bone Stromal Cells In Vitro

2013 ◽  
Vol 3 (5) ◽  
pp. 554-563 ◽  
Author(s):  
Li-Hua Li ◽  
Ying-Hu Deng ◽  
Jin He ◽  
Guo-Bo Lan ◽  
Mei Li ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Stromal Cells ◽  
Cell Tumor ◽  
Biological Feature

Development of osteoplastic Material Impregnated with Plasmid Encoding Bone Morphogenetic Protein-2

2020 ◽  
Vol 36 (4) ◽  
pp. 59-64
Author(s):  
T.B. Bukharova ◽  
Yu.D. Zagoskin ◽  
A.V. Vasilyev ◽  
T.E. Grigoriev ◽  
V.O. Mokrousova ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Biological Properties ◽  
Bone Morphogenetic Protein 2 ◽  
Russian Science ◽  
Morphogenetic Protein ◽  
Osteoplastic Material

The biological properties of a new gene-activated osteoplastic material based on chitosan sponges impregnated with plasmids with the gene for the bone morphogenetic protein-2 (BMP2) have been studied. The optimal conditions for transfection of multipotent mesenchymal stromal cells with TurboFect reagent were selected. The in vitro osteoinducing effect of the developed material associated with an increase in the expression of osteogenic markers in mesenchymal stromal cells was shown. The designed gene-activated material can be used to repair bone defects. gene-activated material, plasmids, BMP-2, gene therapy, bone regeneration The work was supported by the Russian Science Foundation (Grant no. 16-15-00298).

Design of hydroxyapatite bioceramics with micro-/nano-topographies to regulate the osteogenic activities of bone morphogenetic protein-2 and bone marrow stromal cells

Nanoscale ◽  
2020 ◽  
Vol 12 (13) ◽  
pp. 7284-7300 ◽  
Author(s):  
Xiangfeng Li ◽  
Minjun Liu ◽  
Fuying Chen ◽  
Yuyi Wang ◽  
Menglu Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Natural Bone ◽  
Morphogenetic Protein ◽  
Bone Apatite

Biomimicking the nanostructure of natural bone apatite to enhance the bioactivity of hydroxyapatite (HA) biomaterials is an eternal topic in the bone regeneration field.

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