scholarly journals Genetic stability of a recombinant adenovirus vaccine vector seed library expressing human papillomavirus type 16 E6 and E7 proteins

2015 ◽  
Vol 9 (4) ◽  
pp. 1161-1165 ◽  
Author(s):  
JIE WU ◽  
KE-DA CHEN ◽  
MENG GAO ◽  
GANG CHEN ◽  
SU-FENG JIN ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Genetic Stability ◽  
Recombinant Adenovirus ◽  
Vaccine Vector ◽  
Human Papillomavirus Type 16 ◽  
Adenovirus Vaccine ◽  
E6 And E7 ◽  
E7 Proteins

scholarly journals Identification of Antibodies against Human Papillomavirus Type 16 E6 and E7 Proteins in Sera of Patients with Cervical Neoplasias

1992 ◽  
Vol 83 (7) ◽  
pp. 705-713 ◽  
Author(s):  
Toshiyuki Sasagawa ◽  
Masaki Inoue ◽  
Osamu Tanizawa ◽  
Masuo Yutsudo ◽  
Akira Hakura
Keyword(s):  
Human Papillomavirus ◽  
Human Papillomavirus Type 16 ◽  
E6 And E7 ◽  
E7 Proteins

scholarly journals Identification of a new promoter in the early region of the human papillomavirus type 16 genome

1999 ◽  
Vol 80 (12) ◽  
pp. 3241-3250 ◽  
Author(s):  
T. H. Braunstein ◽  
B. S. Madsen ◽  
B. Gavnholt ◽  
M. W. Rosenstierne ◽  
C. Koefoed Johnsen ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Control Synthesis ◽  
Start Site ◽  
Late Gene Expression ◽  
Transcription Start ◽  
Transcription Start Sites ◽  
Polycistronic Mrna ◽  
Human Papillomavirus Type 16 ◽  
E6 And E7 ◽  
E7 Proteins

Transcription of the human papillomavirus type 16 (HPV-16) genome is controlled by several promoters; the P97 promoter is considered to be the main one. An additional promoter has been identified within the E7 ORF as well as an antisense promoter just upstream of the L2 ORF. The significance of these promoters for early and late gene expression and their activity related to cell differentiation is not known in detail. Identification of two new, previously undescribed transcription start sites at nt 542 just upstream of the E7 ORF and at nt 611 within the E7 ORF is reported. The promoter responsible for the start site at nt 542 (P542) was active in SiHa, HeLa and C33A cells. Very low promoter activity was found upstream of the nt 611 start site. The E7 protein has previously been shown to be synthesized from a polycistronic mRNA encoding both the E6 and E7 proteins under the control of the P97 promoter. The data reported in the present paper suggest that promoter P542 may control synthesis of the E7 oncoprotein from a monocistronic mRNA.

Antibodies against Human Papillomavirus Type 16 and 18 E6 and E7 Proteins in Cervicovaginal Washings and Serum of Patients with Cervical Neoplasia

2001 ◽  
Vol 14 (4) ◽  
pp. 415-424 ◽  
Author(s):  
M.Y. Tjiong ◽  
K. Zumbach ◽  
J. ter Schegget ◽  
N. van der Vange ◽  
T.A. Out ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cervical Neoplasia ◽  
Human Papillomavirus Type 16 ◽  
E6 And E7 ◽  
E7 Proteins

Serologic response to the E4, E6, and E7 proteins of human papillomavirus type 16 in pregnant women

1998 ◽  
Vol 178 (2) ◽  
pp. 360-364 ◽  
Author(s):  
Shneh Sethi ◽  
Martin Müller ◽  
Achim Schneider ◽  
Maria Blettner ◽  
Elaine Smith ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Pregnant Women ◽  
Human Papillomavirus Type 16 ◽  
Serologic Response ◽  
E6 And E7 ◽  
E7 Proteins

E6 and E7 Oncoproteins from Human Papillomavirus Type 16 Induce Activation of Human Transforming Growth Factorβ1Promoter throughout Sp1 Recognition Sequence

2006 ◽  
Vol 19 (3) ◽  
pp. 468-480 ◽  
Author(s):  
Oscar Peralta-Zaragoza ◽  
Víctor Bermúdez-Morales ◽  
Lourdes Gutiérrez-Xicotencatl ◽  
Juan Alcocer-González ◽  
Félix Recillas-Targa ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Recognition Sequence ◽  
Human Papillomavirus Type 16 ◽  
E6 And E7 Oncoproteins ◽  
E6 And E7

scholarly journals Multiple ASF/SF2 Sites in the Human Papillomavirus Type 16 (HPV-16) E4-Coding Region Promote Splicing to the Most Commonly Used 3′-Splice Site on the HPV-16 Genome

2010 ◽  
Vol 84 (16) ◽  
pp. 8219-8230 ◽  
Author(s):  
Monika Somberg ◽  
Stefan Schwartz
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Splice Site ◽  
Binding Sites ◽  
Mrna Levels ◽  
Coding Region ◽  
Cervical Lesions ◽  
Hpv 16 ◽  
Human Papillomavirus Type 16 ◽  
E6 And E7

ABSTRACT Our results presented here demonstrate that the most abundant human papillomavirus type 16 (HPV-16) mRNAs expressing the viral oncogenes E6 and E7 are regulated by cellular ASF/SF2, itself defined as a proto-oncogene and overexpressed in cervical cancer cells. We show that the most frequently used 3′-splice site on the HPV-16 genome, site SA3358, which is used to produce primarily E4, E6, and E7 mRNAs, is regulated by ASF/SF2. Splice site SA3358 is immediately followed by 15 potential binding sites for the splicing factor ASF/SF2. Recombinant ASF/SF2 binds to the cluster of ASF/SF2 sites. Mutational inactivation of all 15 sites abolished splicing to SA3358 and redirected splicing to the downstream-located, late 3′-splice site SA5639. Overexpression of a mutant ASF/SF2 protein that lacks the RS domain, also totally inhibited the usage of SA3358 and redirected splicing to the late 3′-splice site SA5639. The 15 ASF/SF2 binding sites could be replaced by an ASF/SF2-dependent, HIV-1-derived splicing enhancer named GAR. This enhancer was also inhibited by the mutant ASF/SF2 protein that lacks the RS domain. Finally, silencer RNA (siRNA)-mediated knockdown of ASF/SF2 caused a reduction in spliced HPV-16 mRNA levels. Taken together, our results demonstrate that the major HPV-16 3′-splice site SA3358 is dependent on ASF/SF2. SA3358 is used by the most abundantly expressed HPV-16 mRNAs, including those encoding E6 and E7. High levels of ASF/SF2 may therefore be a requirement for progression to cervical cancer. This is supported by our earlier findings that ASF/SF2 is overexpressed in high-grade cervical lesions and cervical cancer.

Sign in / Sign up

Export Citation Format

Share Document