scholarly journals Pneumocystis jirovecii dihydropteroate synthase gene mutations in a group of HIV-negative immunocompromised patients with Pneumocystis pneumonia

2014 ◽  
Vol 8 (6) ◽  
pp. 1825-1830 ◽  
Author(s):  
YINGJIAO LONG ◽  
CHENG ZHANG ◽  
LI SU ◽  
CHENGLI QUE
Keyword(s):  
Gene Mutations ◽  
Pneumocystis Pneumonia ◽  
Pneumocystis Jirovecii ◽  
Immunocompromised Patients ◽  
Dihydropteroate Synthase ◽  
Hiv Negative

Severity and outcome of HIV-associated Pneumocystis pneumonia containing Pneumocystis jirovecii dihydropteroate synthase gene mutations

AIDS ◽  
2005 ◽  
Vol 19 (8) ◽  
pp. 801-805 ◽  
Author(s):  
Kristina Crothers ◽  
Charles B Beard ◽  
Joan Turner ◽  
Gena Groner ◽  
Melissa Fox ◽  
...  
Keyword(s):  
Gene Mutations ◽  
Pneumocystis Pneumonia ◽  
Pneumocystis Jirovecii ◽  
Dihydropteroate Synthase

scholarly journals Low Prevalence of Pneumocystis pneumonia (PCP) but High Prevalence of Pneumocystis dihydropteroate synthase (dhps) Gene Mutations in HIV-Infected Persons in Uganda

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49991 ◽  
Author(s):  
Steve M. Taylor ◽  
Steven R. Meshnick ◽  
William Worodria ◽  
Alfred Andama ◽  
Adithya Cattamanchi ◽  
...  
Keyword(s):  
Gene Mutations ◽  
Pneumocystis Pneumonia ◽  
Dihydropteroate Synthase ◽  
High Prevalence ◽  
Dhps Gene ◽  
Low Prevalence

Dihydropteroate synthase gene mutations in Pneumocystis jiroveci strains isolated from immunocompromised patients

2011 ◽  
Vol 59 (4) ◽  
pp. 222-225 ◽  
Author(s):  
M.A. Jarboui ◽  
A. Sellami ◽  
H. Sellami ◽  
F. Cheikhrouhou ◽  
F. Makni ◽  
...  
Keyword(s):  
Gene Mutations ◽  
Immunocompromised Patients ◽  
Pneumocystis Jiroveci ◽  
Dihydropteroate Synthase

Detection of Pneumocystis jirovecii dihydropteroate synthase polymorphisms in patients with Pneumocystis pneumonia

2005 ◽  
Vol 37 (10) ◽  
pp. 766-771 ◽  
Author(s):  
M. C Costa ◽  
J Gaspar ◽  
K Mansinho ◽  
F Esteves ◽  
F Antunes ◽  
...  
Keyword(s):  
Pneumocystis Pneumonia ◽  
Pneumocystis Jirovecii ◽  
Dihydropteroate Synthase

scholarly journals Development of a Droplet Digital Polymerase Chain Reaction for Sensitive Detection of Pneumocystis jirovecii in Respiratory Tract Specimens

2021 ◽  
Vol 8 ◽  
Author(s):  
Jie Yi ◽  
Nan Wang ◽  
Jie Wu ◽  
Yueming Tang ◽  
Jingjia Zhang ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Pneumocystis Pneumonia ◽  
Pneumocystis Jirovecii ◽  
Immunocompromised Patients ◽  
Balf Sample ◽  
Bronchoalveolar Fluid ◽  
Life Threatening ◽  
Digital Polymerase Chain Reaction

Background:Pneumocystis jirovecii is a human-specific opportunistic fungus that causes Pneumocystis pneumonia (PCP), a life-threatening opportunistic lung infection that affects immunocompromised patients. P. jirovecii colonization may be linked to the transmission of the infection. The detection of P. jirovecii in immunocompromised patients is thus especially important. The low fungal load and the presence of PCR inhibitors limit the usefulness of quantitative PCR (qPCR) for accurate absolute quantification of P. jirovecii in specimens. Droplet digital PCR (ddPCR), however, presents a methodology that allows higher sensitivity and accuracy. Here, we developed a ddPCR method for detecting P. jirovecii DNA in respiratory specimens, and evaluated its sensitivity against qPCR.Materials and Methods: One bronchoalveolar fluid (BALF) sample each was collected from 82 patients with potential PCP to test the presence of P. jirovecii DNA using both ddPCR and qPCR, and samples with inconsistent results between the two methods were further tested by metagenomic next generation sequencing (mNGS). In addition, 37 sputum samples from 16 patients diagnosed with PCP, as well as continuous respiratory tract specimens from nine patients with PCP and treated with sulfonamides, were also collected for P. jirovecii DNA testing using both ddPCR and qPCR.Results: ddPCR and qPCR gave the same results for 95.12% (78/82) of the BALF samples. The remaining four specimens tested positive using ddPCR but negative using qPCR, and they were found to be positive by mNGS. Detection results of 78.37% (29/37) sputum samples were consistent between ddPCR and qPCR, while the other eight samples tested positive using ddPCR but negative using qPCR. The P. jirovecii load of patients with PCP decreased to undetectable levels after treatment according to qPCR, but P. jirovecii was still detectable using ddPCR.Conclusions: ddPCR was more sensitive than qPCR, especially at detecting low-pathogen-load P. jirovecii. Thus, ddPCR represents a useful, viable, and reliable alternative to qPCR in P. jirovecii testing in patients with immunodeficiency.

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