scholarly journals Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol

2012 ◽  
Vol 5 (2) ◽  
pp. 479-484 ◽  
Author(s):  
JAROMIR GUMULEC ◽  
MARTINA RAUDENSKA ◽  
MARIAN HLAVNA ◽  
TIBOR STRACINA ◽  
MARKETA SZTALMACHOVA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Guinea Pigs

Oxidative Stress and Antioxidant Enzymes in COPD Associated to Biomass Exposure in Guinea Pigs.

2009 ◽  
Author(s):  
C Ramos ◽  
J Pedraza-Chaverri ◽  
C Becerril ◽  
O Medina-Campos ◽  
J Cisneros ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Guinea Pigs

scholarly journals The Influence of Solar Radiation on the Antioxidant Systems in Blood of Dairy Cows and the Processing of the Data Using Wavelets Transforms

2016 ◽  
Vol 73 (2) ◽  
pp. 314
Author(s):  
Dana Liana Pusta ◽  
Rodica Sobolu ◽  
Sanda Andrei ◽  
Camelia Raducu ◽  
Ioana Pop ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Solar Radiation ◽  
Dairy Cows ◽  
Wavelet Transforms ◽  
Oxidative Status ◽  
Antioxidant Systems ◽  
Hot Environment ◽  
Made In

The purpose of this paper was to observe if in cattle, exposed to the solar radiation, could be noticed a certain reaction of the organism related to the oxidative stress. The study was made in the period May - August 2014, on a group of 16 Romanian Simmental dairy cows, kept on pasture during the day. The processing of the determined data were made using wavelet transforms (In-Place Fast Haar Wavelets Transform). The results of the experiment shown that when Temperature Humidity Indexes (THI) are higher than 72 (the superior limit for thermal comfort in cattle), the oxidative stress appeared in dairy cows. This oxidative stress was mainly manifested by the increasing of superoxide dismutase with 95% in August compared to May, followed by the increasing of the catalase with 79% and of glutathione peroxidase with 13%. The increasing of the antioxidant enzymes level was directly co-related with THI. We considered that the determination of the antioxidant enzymes level was an appropriate model for studying the influence of hot environment on the oxidative status of dairy cows. The wavelets transforms can be easier applied to practical data compared to the classical statistical methods.

scholarly journals Disturbance of Antioxidant Enzymes and Purine Metabolism in the Ejaculate of Men Living in Disadvantaged Areas of Kyzylorda Region

2015 ◽  
Vol 3 (3) ◽  
pp. 489-492
Author(s):  
Valentihna N. Kislitskaya ◽  
Zhandos D. Kenzhin ◽  
Berikbay Zh. Kultanov ◽  
Raushan S. Dosmagambetova ◽  
Anar A. Turmuhambetova
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Adenosine Deaminase ◽  
Purine Metabolism ◽  
Reproductive Age ◽  
Aral Sea ◽  
Hazard Class ◽  
Sea Area ◽  
Radical Processes

AIM: Objective of the study was to evaluate the state of the main indicators of antioxidant status and enzymes of purine metabolism in the germ cells of men living in the zone of ecological catastrophe Aral Sea region.METHODS: The criterion for inclusion is the stay of an adult in the Aral Sea area is not less than 5 years, employment in occupations with no more than 2 hazard class. Determination of the activity of adenosine deaminase (ADA) was conducted in semen by the method of Nemechek et al., 1993. Determination of the activity of catalase (CAT) was performed according by the method of Korolyuk et al., 1988.RESULTS: Results of the study indicate a change in the activity of catalase and adenosine deaminase, due to increased levels of oxidative stress and the development of the pathological process.CONCLUSIONS: According to the results of study, it was put the influence  of negative factors of the Aral Sea region in men’s sperm of reproductive  age gives to disability free-radical processes, that proves changing of ferments of ant oxidative protection Catalase and adenosine deaminase (ADA).  This disturbance in men’s sperm of reproductive age leading to increased level of oxidative stress and impaired activity of  antioxidant enzymes and purine metabolism, responsible for the abnormal transmembrane and intracellular processes, reflecting the degree of imbalance of enzymes.

Assessment of Superoxide Dismutase and Catalase Evolution in Different Stages of an Experimental Endometriosis

2017 ◽  
Vol 68 (6) ◽  
pp. 1381-1383
Author(s):  
Allia Sindilar ◽  
Carmen Lacramioara Zamfir ◽  
Eusebiu Viorel Sindilar ◽  
Alin Constantin Pinzariu ◽  
Eduard Crauciuc ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Reproductive Function ◽  
Female Rats ◽  
Oxygen Species ◽  
Efficient Treatment ◽  
Endometrial Cells ◽  
Gynecological Disorder ◽  
The Impact

Endometriosis is described as a gynecological disorder characterized by the presence of endometrial tissue outside the uterus; extensively explored because of its increasing incidency, with an indubitable diagnostic only after invasive surgery, with no efficient treatment, it has still many aspects to be elucidated. A growing body of facts sustain oxidative stress as a crucial factor between the numerous incriminated factors implicated in endometriosis ethiopathogeny. Reactive oxygen species(ROS) act to decline reproductive function. Our study intends to determine if an experimental model of endometriosis may be useful to assess the impact of oxidative stress on endometrial cells; we have used a murine model of 18 adult Wistar female rats. A fragment from their left uterine horn was implanted in the abdominal wall. After 4 weeks, a laparatomy was performed, 5 endometrial implants were removed, followed by biochemical tissue assay of superoxide dismutase(SOD) and catalase(CAT). At the end of the experiment, the rats were sacrificed, the implants were removed for histopathological exam and biochemical assay of antioxidant enzymes. The results revealed decreased levels of antioxidant enzymes, pointing on significant oxidative stress involvement.

Alteration in the oxidative status of Drosophila melanogaster Meigen (Diptera: Drosophilidae) fed with a diet containing Centaurea depressa M. Bieb. (Asteraceae)

2020 ◽  
Vol 70 (2) ◽  
pp. 227-237
Author(s):  
Eda Güneş
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Total Protein ◽  
Protein Carbonyl ◽  
Control Group ◽  
Carbonyl Content ◽  
Protein Carbonyl Content ◽  
Freeze Dried ◽  
Dried Extract

Abstract The aim of the this study was to evaluate the effects of fresh, dried and freeze-dried Centaurea depressa M. Bieb. (Asteraceae) on the oxidant and antioxidant status of the model organism D. melanogaster Meigen (Diptera: Drosophilidae) experimentally. The study was carried out from 2016 to 2019, and plant leaf extracts (0-50 mg/l) were added to insect standard artificial diets. The total protein, protein carbonyl content and glutathione-S-transferase, superoxide dismutase and catalase activities were quantified at the insect’s third larval stage. Our data showed that protein carbonyl content varied from 2.70 nmol/mg protein in the control group to 59.11 nmol/mg protein in the group fed with fresh leaf extract signifying induction of oxidative stress. All extracts increased the levels of all antioxidant enzymes and decreased the amounts of total protein. Meanwhile, the group fed with the freeze-dried extract showed no significant difference in the levels of total protein and protein carbonyl content except at the 50 mg/l concentration of the extract. Moreover, this group had superoxide dismutase and catalase activities 4 to 5 times higher than in the control group. In conclusion, induction of oxidative stress indicates that the fresh form of C. depressa leaves may have potential as a natural pesticide, whereas induction of endogenous antioxidant enzymes by the freeze-dried extract suggest its potential as an antioxidant.

scholarly journals Electroanalytical Techniques for the Detection of Selenium as a Biologically and Environmentally Significant Analyte—A Short Review

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1768
Author(s):  
Miroslav Rievaj ◽  
Eva Culková ◽  
Damiána Šandorová ◽  
Zuzana Lukáčová-Chomisteková ◽  
Renata Bellová ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Essential Element ◽  
Analytical Techniques ◽  
High Sensitivity ◽  
Stripping Voltammetry ◽  
Short Review ◽  
Electroanalytical Methods ◽  
High Concentrations ◽  
Speed Of Analysis

This short review deals with the properties and significance of the determination of selenium, which is in trace amounts an essential element for animals and humans, but toxic at high concentrations. It may cause oxidative stress in cells, which leads to the chronic disease called selenosis. Several analytical techniques have been developed for its detection, but electroanalytical methods are advantageous due to simple sample preparation, speed of analysis and high sensitivity of measurements, especially in the case of stripping voltammetry very low detection limits even in picomoles per liter can be reached. A variety of working electrodes based on mercury, carbon, silver, platinum and gold materials were applied to the analysis of selenium in various samples. Only selenium in oxidation state + IV is electroactive therefore the most of voltammetric determinations are devoted to it. However, it is possible to detect also other forms of selenium by indirect electrochemistry approach.

Exogenous 24-Epibrassinolide stimulates root protection, and leaf antioxidant enzymes in rice plants lead stressed: Central roles to minimize Pb content and oxidative stress

2021 ◽  
pp. 116992
Author(s):  
Flávia Raphaela Carvalho Miranda Guedes ◽  
Camille Ferreira Maia ◽  
Breno Ricardo Serrão da Silva ◽  
Bruno Lemos Batista ◽  
Mohammed Nasser Alyemeni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Rice Plants ◽  
Root Protection ◽  
And Oxidative Stress

scholarly journals Pathophysiological In Vitro Profile of Neuronal Differentiated Cells Derived from Niemann-Pick Disease Type C2 Patient-Specific iPSCs Carrying the NPC2 Mutations c.58G>T/c.140G>T

2021 ◽  
Vol 22 (8) ◽  
pp. 4009
Author(s):  
Maik Liedtke ◽  
Christin Völkner ◽  
Alexandra V. Jürs ◽  
Franziska Peter ◽  
Michael Rabenstein ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transmembrane Protein ◽  
Hereditary Disease ◽  
Cholesterol Homeostasis ◽  
Patient Specific ◽  
Differentiated Cells ◽  
Npc1 Gene ◽  
Niemann Pick

Niemann-Pick type C2 (NP-C2) disease is a rare hereditary disease caused by mutations in the NPC2 gene. NPC2 is a small, soluble protein consisting of 151 amino acids, primarily expressed in late endosomes and lysosomes (LE/LY). Together with NPC1, a transmembrane protein found in these organelles, NPC2 accomplishes the exclusion of cholesterol; thus, both proteins are essential to maintain cellular cholesterol homeostasis. Consequently, mutations in the NPC2 or NPC1 gene result in pathophysiological accumulation of cholesterol and sphingolipids in LE/LY. The vast majority of Niemann-Pick type C disease patients, 95%, suffer from a mutation of NPC1, and only 5% display a mutation of NPC2. The biochemical phenotype of NP-C1 and NP-C2 appears to be indistinguishable, and both diseases share several commonalities in the clinical manifestation. Studies of the pathological mechanisms underlying NP-C2 are mostly based on NP-C2 animal models and NP-C2 patient-derived fibroblasts. Recently, we established induced pluripotent stem cells (iPSCs), derived from a donor carrying the NPC2 mutations c.58G>T/c.140G>T. Here, we present a profile of pathophysiological in vitro features, shared by NP-C1 and NP-C2, of neural differentiated cells obtained from the patient specific iPSCs. Profiling comprised a determination of the NPC2 protein level, detection of cholesterol accumulation by filipin staining, analysis of oxidative stress, and determination of autophagy. As expected, the NPC2-deficient cells displayed a significantly reduced amount of NPC2 protein, and, accordingly, we observed a significantly increased amount of cholesterol. Most notably, NPC2-deficient cells displayed only a slight increase of reactive oxygen species (ROS), suggesting that they do not suffer from oxidative stress and express catalase at a high level. As a site note, comparable NPC1-deficient cells suffer from a lack of catalase and display an increased level of ROS. In summary, this cell line provides a valuable tool to gain deeper understanding, not only of the pathogenic mechanism of NP-C2, but also of NP-C1.

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