scholarly journals Serum‑deprivation leads to activation‑like changes in primary microglia and BV‑2 cells but not astrocytes

2020 ◽  
Vol 13 (5) ◽  
pp. 1-1
Author(s):  
Yao Yao ◽  
Kai-Yuan Fu
Keyword(s):  
Serum Deprivation ◽  
Primary Microglia

scholarly journals GPR110 ligands reduce chronic optic tract gliosis and visual deficit following repetitive mild traumatic brain injury in mice

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Huazhen Chen ◽  
Karl Kevala ◽  
Elma Aflaki ◽  
Juan Marugan ◽  
Hee-Yong Kim
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Mild Traumatic Brain Injury ◽  
Visual Evoked Potential ◽  
Evoked Potential ◽  
Optic Tract ◽  
Primary Microglia ◽  
Visual Dysfunction ◽  
The Brain

Abstract Background Repetitive mild traumatic brain injury (mTBI) can result in chronic visual dysfunction. G-protein receptor 110 (GPR110, ADGRF1) is the target receptor of N-docosahexaenoylethanolamine (synaptamide) mediating the anti-neuroinflammatory function of synaptamide. In this study, we evaluated the effect of an endogenous and a synthetic ligand of GPR110, synaptamide and (4Z,7Z,10Z,13Z,16Z,19Z)-N-(2-hydroxy-2-methylpropyl) docosa-4,7,10,13,16,19-hexaenamide (dimethylsynaptamide, A8), on the mTBI-induced long-term optic tract histopathology and visual dysfunction using Closed-Head Impact Model of Engineered Rotational Acceleration (CHIMERA), a clinically relevant model of mTBI. Methods The brain injury in wild-type (WT) and GPR110 knockout (KO) mice was induced by CHIMERA applied daily for 3 days, and GPR110 ligands were intraperitoneally injected immediately following each impact. The expression of GPR110 and proinflammatory mediator tumor necrosis factor (TNF) in the brain was measured by using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) in an acute phase. Chronic inflammatory responses in the optic tract and visual dysfunction were assessed by immunostaining for Iba-1 and GFAP and visual evoked potential (VEP), respectively. The effect of GPR110 ligands in vitro was evaluated by the cyclic adenosine monophosphate (cAMP) production in primary microglia isolated from adult WT or KO mouse brains. Results CHIMERA injury acutely upregulated the GPR110 and TNF gene level in mouse brain. Repetitive CHIMERA (rCHIMERA) increased the GFAP and Iba-1 immunostaining of glia cells and silver staining of degenerating axons in the optic tract with significant reduction of N1 amplitude of visual evoked potential at up to 3.5 months after injury. Both GPR110 ligands dose- and GPR110-dependently increased cAMP in cultured primary microglia with A8, a ligand with improved stability, being more effective than synaptamide. Intraperitoneal injection of A8 at 1 mg/kg or synaptamide at 5 mg/kg significantly reduced the acute expression of TNF mRNA in the brain and ameliorated chronic optic tract microgliosis, astrogliosis, and axonal degeneration as well as visual deficit caused by injury in WT but not in GPR110 KO mice. Conclusion Our data demonstrate that ligand-induced activation of the GPR110/cAMP system upregulated after injury ameliorates the long-term optic tract histopathology and visual impairment caused by rCHIMERA. Based on the anti-inflammatory nature of GPR110 activation, we suggest that GPR110 ligands may have therapeutic potential for chronic visual dysfunction associated with mTBI.

Selenium promotes proliferation of chondrogenic cell ATDC5 by increment of intracellular ATP content under serum deprivation

2012 ◽  
Vol 30 (8) ◽  
pp. 657-663 ◽  
Author(s):  
Jidong Yan ◽  
Juan Tian ◽  
Yuewen Zheng ◽  
Yan Han ◽  
Shemin Lu
Keyword(s):  
Serum Deprivation ◽  
Atp Content ◽  
Intracellular Atp

scholarly journals Hypoxia-induced VEGF enhances tumor survivability via suppression of serum deprivation-induced apoptosis

Oncogene ◽  
2000 ◽  
Vol 19 (40) ◽  
pp. 4621-4631 ◽  
Author(s):  
Jin Hyen Baek ◽  
Jae-Eun Jang ◽  
Chang-Mo Kang ◽  
Hae-Young Chung ◽  
Nam Deuk Kim ◽  
...  
Keyword(s):  
Serum Deprivation ◽  
Induced Apoptosis

bFGF expression mediated by a hypoxia-regulated adenoviral vector protects PC12 cell death induced by serum deprivation

2009 ◽  
Vol 390 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Hou-Wen Hu ◽  
Xiao-Kun Li ◽  
Rong-Yuan Zheng ◽  
Jian Xiao ◽  
Jin-Qi Zeng ◽  
...  
Keyword(s):  
Cell Death ◽  
Pc12 Cell ◽  
Adenoviral Vector ◽  
Serum Deprivation

Punicalagin inhibits neuroinflammation in LPS-activated rat primary microglia

2014 ◽  
Vol 58 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
Olumayokun A. Olajide ◽  
Asit Kumar ◽  
Ravikanth Velagapudi ◽  
Uchechukwu P. Okorji ◽  
Bernd L. Fiebich
Keyword(s):  
Primary Microglia

scholarly journals Adenosine Triphosphate Prevents Serum Deprivation-Induced Apoptosis in Human Mesenchymal Stem Cells via Activation of the MAPK Signaling Pathways

Stem Cells ◽  
2014 ◽  
Vol 33 (1) ◽  
pp. 211-218 ◽  
Author(s):  
Jessica L. Berlier ◽  
Sabrina Rigutto ◽  
Antoine Dalla Valle ◽  
Jessica Lechanteur ◽  
Muhammad S. Soyfoo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathways ◽  
Adenosine Triphosphate ◽  
Human Mesenchymal Stem Cells ◽  
Mapk Signaling ◽  
Serum Deprivation ◽  
Induced Apoptosis ◽  
Mapk Signaling Pathways
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