scholarly journals Association between host TNF‑α, TGF‑β1, p53 polymorphisms, HBV X gene mutation, HBV viral load and the progression of HBV‑associated chronic liver disease in Indonesian patients

2019 ◽  
Author(s):  
Citrawati Dyah Kencono Wungu ◽  
Mochamad Amin ◽  
S. Eriaty N. Ruslan ◽  
Priyo Purwono ◽  
Ulfa Kholili ◽  
...  
Keyword(s):  
Viral Load ◽  
Liver Disease ◽  
Chronic Liver Disease ◽  
Gene Mutation ◽  
Chronic Liver ◽  
Tnf Α ◽  
X Gene ◽  
Tgf Β1 ◽  
P53 Polymorphisms

scholarly journals Prolonged Detection of SARS CoV2 RNA in Extracellular Vesicles in Nasal Swab RT-PCR Negative Patients

2021 ◽  
Author(s):  
P Debishree Subudhi ◽  
Sheetalnath Rooge ◽  
Swati Thangriyal ◽  
Reshu Aggarwal ◽  
Ekta Gupta ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Viral Load ◽  
Liver Disease ◽  
Chronic Liver Disease ◽  
Extracellular Vesicles ◽  
Nasal Swab ◽  
High Viral Load ◽  
Chronic Liver ◽  
Rt Pcr ◽  
Route Of Transmission

Background: There is a prolonged RT PCR positivity seen in COVID-19 infected patients up to 2 to 3 months. It is assumed that this virus is usually non-infective but there are hardly any study on the reactivation of this virus within the respiratory tract. We aim to investigate the presence of viral particles inside Extracellular vesicles (EV) and its role in underlying liver disease patients. Methods: SARS CoV2 nasal and throat swab RT-PCR positive n=78 {n=24(66.6%) chronic liver disease (CLD); n=52 (81.3%) non liver disease} n=5 RT PCR negative subjects (HC) were studied. SARS CoV2 patients were also followed up for day (d) 7, 14 and 28. Nasal swab [collected in viral transport media (VTM)] and plasma samples were investigated at each time point. Extracellular vesicles were isolated using differential ultracentrifugation. SARS CoV2 RNA was measured using qRT-PCR by Altona Real Star kit. Cellular origin of EV was confirmed using epithelial cells (Epcam+ CK19+ CDh1+), endothelial cells (CD31+CD45-), and hepatocytes (ASGPR+) surface markers by Flow cytometry. Results: The COVID19 patients {Mean age 54±23 years; 41 males} were having severity between moderate to severe. In patients with cirrhosis, the most common aetiology of liver disease was alcohol (MELD 22±8). In baseline RT-PCR positive patients, SARS-CoV2 RNA inside the EV was present in 64/74 (82%) patients with comparable viral load between VTM and EV (mean 1/CT 0.033±0.005 vs. 1/CT 0.029±0.014, p=ns). On follow-up at day 7, of the 24 patients negative for COVID19, 10 (41%) had persistence of virus in the EV (1/CT 0.028±0.004) and on day 14, 14 of 40 (35%) negative RT-PCR had EVs with SARS CoV2 RNA (1/CT 0.028±0.06). The mean viral load decreased at day7 and day14 in nasal swab from baseline (p=0.001) but not in EV. SARS-CoV2 RNA otherwise undetectable in plasma, was found to be positive in EV in 12.5% of COVID19 positive patients. Interestingly, significantly prolonged and high viral load was found in EV at day 14 in CLD COVID19 patients compared to COVID19 alone (p=0.002). The high cellular injury was seen in CLD COVID19 infected patients with significant high levels of EV associated with endothelial cells and hepatocytes than COVID19 alone (p=0.004; 0.001). Conclusion: Identification of SARS-CoV2 RNA in EV, in RT-PCR negative patients indicates persistence of infection for and likely recurrence of the infection. It is suggestive of another route of transmission as EV harbour SARS CoV2 RNA. EV associated RNA may determine the ongoing inflammation and clinical course of subjects with undetectable SARS-CoV2 virus and this may also have relevance in management of chronic liver disease patients.

Past HBV Viral Load as Predictor of Mortality and Morbidity from HCC and Chronic Liver Disease in a Prospective Study

2006 ◽  
Vol 101 (8) ◽  
pp. 1797-1803 ◽  
Author(s):  
Gang Chen ◽  
Wenyao Lin ◽  
Fumin Shen ◽  
Uchenna H. Iloeje ◽  
W. Thomas London ◽  
...  
Keyword(s):  
Viral Load ◽  
Liver Disease ◽  
Prospective Study ◽  
Chronic Liver Disease ◽  
Chronic Liver ◽  
Mortality And Morbidity ◽  
A Prospective Study

TNF-α and Growth Hormone Resistance in Patients with Chronic Liver Disease

2003 ◽  
Vol 23 (5) ◽  
pp. 229-235 ◽  
Author(s):  
Antonio Picardi ◽  
Umberto Vespasiani Gentilucci ◽  
Enrico Maria Zardi ◽  
Domenico Caccavo ◽  
Tommasangelo Petitti ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Liver Disease ◽  
Chronic Liver Disease ◽  
Chronic Liver ◽  
Hormone Resistance ◽  
Growth Hormone Resistance ◽  
Tnf Α

scholarly journals Biochemical Studies of Matrix Metalloproteinase (MMP) and tissue inhibitor of Metalloproteinase (TIMP) as New Biomarkers in HCV - Chronic liver disease

2017 ◽  
Vol 1 (4) ◽  
Author(s):  
Elgzar M. Y., Elemshaty M.H., Amar E.A.T., Abdel Atty A.M.,
Keyword(s):  
Liver Disease ◽  
Matrix Metalloproteinase ◽  
Chronic Liver Disease ◽  
Group Iv ◽  
Chronic Liver ◽  
Hcv Infection ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Tissue Inhibitor ◽  
Tnf Α ◽  
New Biomarkers

The present study aimed to investigate serum levels of Matrix Metalloproteinase (MMP9) and tissue inhibitor of Metalloproteinase (TIMP-1) as new biomarkers in HCV Chronic liver disease. Patients and methods: The study included 35 patients suffering from Chronic liver disease attending at Gastro Entrology Surgical Center (GEC), Mansoura University from 2016 to 2017 and 25 healthy individuals (HI) used as control group ,the studied groups were categorized into 14 patients (9 men and 5women; mean age 53.34±9.46yrs) suffering from HCC associated with HCV infection, 11patients (9 men and 2 women; mean age 52.34±9.14yrs) suffering from LC associated with HCV infection and 10 patients (7 men and 3women; mean age 48.9 ± 12.4yrs) suffering from Chronic Hepatitis (CH). Sera of all individuals were examined for TNF-α, LDH, AFP, ALT, AST, Albumin, T.Bilirubin, GGT, MMP-9 and TIMP-1 .  Results: The obtained results showed a significant increase in serum ALT, AST, total Bilirubin and GGT and significant decrease of Albumin in liver disease groups (I, II and III) compared with Group IV. Significant increase in serum MMP9, TIMP1 in groups (I, II and III) when compared with HI group (IV). Significant correlation was recorded between MMP9 and TIMP1 in one hand and between each one with AST, T. bilirubin and Albumin concentration. Serum concentrations of TNF-α, AFP were elevated significantly in HCC patients compared to LC and CH but the difference between LC and CH was elevated significantly only (p<0.0001) in TNF-α. Significant association was recorded between TNF-α, LDH, AFP, ALT and AST Conclusion: MMP9 and TIMP1 could be used as new biomarker for evaluating disease progression of HCV-chronic liver disease

HFE Gene Mutation, Chronic Liver Disease, and Iron Overload In Turkey

2007 ◽  
Vol 52 (11) ◽  
pp. 3298-3302 ◽  
Author(s):  
Oya Yönal ◽  
Özden Hatırnaz ◽  
Filiz Akyüz ◽  
Ugur Özbek ◽  
Kadir Demir ◽  
...  
Keyword(s):  
Liver Disease ◽  
Iron Overload ◽  
Chronic Liver Disease ◽  
Gene Mutation ◽  
Chronic Liver ◽  
Hfe Gene ◽  
Hfe Gene Mutation

scholarly journals Serum cytokines, adipokines and ferritin for non-invasive assessment of liver fibrosis in chronic liver disease: a systematic review

2019 ◽  
Vol 57 (5) ◽  
pp. 577-610 ◽  
Author(s):  
Seyyed Mortaza Haghgoo ◽  
Heidar Sharafi ◽  
Seyed Moayed Alavian
Keyword(s):  
Chronic Hepatitis ◽  
Liver Disease ◽  
Growth Factor ◽  
Liver Fibrosis ◽  
Chronic Liver Disease ◽  
Serum Levels ◽  
Chronic Liver ◽  
Major Health Problem ◽  
Non Invasive ◽  
Tgf Β1

AbstractChronic liver disease (CLD) is a major health problem worldwide. Non-alcoholic fatty liver disease (NAFLD), chronic hepatitis C (CHC), chronic hepatitis B (CHB), and alcoholic liver disease (ALD) are the most common etiologies of CLD. Liver biopsy is the gold standard for assessment of liver fibrosis, however, it is an invasive method. This review attempts to evaluate the usefulness of serum adiponectin, serum leptin, serum ferritin, serum transforming growth factor-β1 (TGF-β1), and serum platelet derived growth factor-BB (PDGF-BB) as non-invasive markers in the diagnosis of liver fibrosis/cirrhosis. A systematic search in MEDLINE, Web of Science, Scopus, and local databases was performed to identify articles published in English or Persian as of November 2017. Studies conducted among CLD patients, with biopsy proven fibrosis/cirrhosis, and providing sufficient details of patients’ clinicopathological characteristics were included. In the 95 studies included, there were a total of 15,548 CLD patients. More than 83% of studies were carried out in Asia and Europe. The relationship between liver fibrosis/cirrhosis and serum levels of ferritin, adiponectin, leptin, TGF-β1, and PDGF-BB was assessed in 42, 33, 27, nine, and three studies, respectively. Serum levels of the markers, particularly ferritin, could successfully predict liver fibrosis/cirrhosis, however, these data might not be clinically replicated and further studies are needed.

scholarly journals Detection of hepatitis B virux X-gene protein and anti-X in type B chronic liver disease.

Kanzo ◽  
1988 ◽  
Vol 29 (4) ◽  
pp. 483-487
Author(s):  
Kazuhiro KATAYAMA ◽  
Norio HAYASHI ◽  
Yutaka SASAKI ◽  
Akinori KASAHARA ◽  
Keiji UEDA ◽  
...  
Keyword(s):  
Liver Disease ◽  
Hepatitis B ◽  
Chronic Liver Disease ◽  
Chronic Liver ◽  
Type B ◽  
X Gene

300 SERPINB3 AND TGF-β1 CORRELATION IN CHRONIC LIVER DISEASE

2009 ◽  
Vol 50 ◽  
pp. S117
Author(s):  
C. Turato ◽  
F. Calabrese ◽  
A. Biasiolo ◽  
S. Quarta ◽  
M. Ruvoletto ◽  
...  
Keyword(s):  
Liver Disease ◽  
Chronic Liver Disease ◽  
Chronic Liver ◽  
Tgf Β1
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