scholarly journals Cell culture density affects the stemness gene expression of adipose tissue-derived mesenchymal stem cells

2017 ◽  
Vol 6 (3) ◽  
pp. 300-306 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Tae-Hee Lee ◽  
Ki Woong Sung ◽  
Hong Hoe Koo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Culture Density

scholarly journals Gene Expression Profiles of Human Adipose Tissue-Derived Mesenchymal Stem Cells Are Modified by Cell Culture Density

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e83363 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Keon Hee Yoo ◽  
Tae-Hee Lee ◽  
Hye Jin Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Adipose Tissue ◽  
Culture Density

Cell culture density affects the proliferation activity of human adipose tissue stem cells

2016 ◽  
Vol 34 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Young Jong Ko ◽  
Yong Hoon Chun ◽  
Hyung Joon Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Culture Density ◽  
Proliferation Activity

scholarly journals Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

2016 ◽  
Vol 33 (10) ◽  
pp. 1395-1403 ◽  
Author(s):  
Moysés S. Miranda ◽  
Hamilton S. Nascimento ◽  
Mayra P. R. Costa ◽  
Nathália N. Costa ◽  
Karynne N. L. Brito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Bovine Embryos ◽  
Blastocyst Rate

Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue

2019 ◽  
Vol 120 (7) ◽  
pp. 11842-11852 ◽  
Author(s):  
Simone Ortiz Moura Fideles ◽  
Adriana Cassia Ortiz ◽  
Amanda Freire Assis ◽  
Max Jordan Duarte ◽  
Fabiola Singaretti Oliveira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteoblast Differentiation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cell Source

scholarly journals Influence of Different ECM-Like Hydrogels on Neurite Outgrowth Induced by Adipose Tissue-Derived Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
E. Oliveira ◽  
R. C. Assunção-Silva ◽  
O. Ziv-Polat ◽  
E. D. Gomes ◽  
F. G. Teixeira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Growth Factors ◽  
Axonal Outgrowth ◽  
Mrna Levels ◽  
Cord Injury

Mesenchymal stem cells (MSCs) have been proposed for spinal cord injury (SCI) applications due to their capacity to secrete growth factors and vesicles—secretome—that impacts important phenomena in SCI regeneration. To improve MSC survival into SCI sites, hydrogels have been used as transplantation vehicles. Herein, we hypothesized if different hydrogels could interact differently with adipose tissue-derived MSCs (ASCs). The efficacy of three natural hydrogels, gellan gum (functionalized with a fibronectin peptide), collagen, and a hydrogel rich in laminin epitopes (NVR-gel) in promoting neuritogenesis (alone and cocultured with ASCs), was evaluated in the present study. Their impact on ASC survival, metabolic activity, and gene expression was also evaluated. Our results indicated that all hydrogels supported ASC survival and viability, being this more evident for the functionalized GG hydrogels. Moreover, the presence of different ECM-derived biological cues within the hydrogels appears to differently affect the mRNA levels of growth factors involved in neuronal survival, differentiation, and axonal outgrowth. All the hydrogel-based systems supported axonal growth mediated by ASCs, but this effect was more robust in functionalized GG. The data herein presented highlights the importance of biological cues within hydrogel-based biomaterials as possible modulators of ASC secretome and its effects for SCI applications.

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