scholarly journals β-2 microglobulin is unsuitable as an internal reference gene for the analysis of gene expression in human colorectal cancer

2013 ◽  
Vol 1 (2) ◽  
pp. 193-196 ◽  
Author(s):  
YASUHIRO NIHON-YANAGI ◽  
KENSUKE TERAI ◽  
TAKEYOSHI MURANO ◽  
TAKAYUKI KAWAI ◽  
SHINYA KIMURA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Reference Gene ◽  
Human Colorectal Cancer ◽  
Internal Reference ◽  
Internal Reference Gene

scholarly journals Validation of internal reference genes for relative quantitation studies of gene expression in human laryngeal cancer

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2763 ◽  
Author(s):  
Xiaofeng Wang ◽  
Jinting He ◽  
Wei Wang ◽  
Ming Ren ◽  
Sujie Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Laryngeal Cancer ◽  
Reference Genes ◽  
Target Gene ◽  
Cancer Tissue ◽  
Internal Reference ◽  
Target Gene Expression ◽  
Internal Reference Gene ◽  
Relative Quantitation

BackgroundThe aim of this study was to determine the expression stabilities of 12 common internal reference genes for the relative quantitation analysis of target gene expression performed by reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human laryngeal cancer.MethodsHep-2 cells and 14 laryngeal cancer tissue samples were investigated. The expression characteristics of 12 internal reference gene candidates (18S rRNA, GAPDH, ACTB, HPRT1, RPL29, HMBS, PPIA, ALAS1, TBP, PUM1, GUSB, and B2M) were assessed by RT-qPCR. The data were analyzed by three commonly used software programs: geNorm, NormFinder, and BestKeeper.ResultsThe use of the combination of four internal reference genes was more appropriate than the use of a single internal reference gene. The optimal combination was PPIA + GUSB + RPL29 + HPRT1 for both the cell line and tissues; while the most appropriate combination was GUSB + RPL29 + HPRT1 + HMBS for the tissues.ConclusionsOur recommended internal reference genes may improve the accuracy of relative quantitation analysis of target gene expression performed by the RT-qPCR method in further gene expression research on laryngeal tumors.

Prognostic significance of Bcl-xL gene expression in human colorectal cancer

2011 ◽  
Vol 113 (8) ◽  
pp. 810-814 ◽  
Author(s):  
Yang Jin-Song ◽  
Wang Zhao-Xia ◽  
Lv Cheng-Yu ◽  
Liang Xiao-Di ◽  
Sun Ming ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Prognostic Significance ◽  
Human Colorectal Cancer

scholarly journals Effects of olive oil polyphenols on fatty acid synthase gene expression and activity in human colorectal cancer cells

2010 ◽  
Vol 6 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Maria Notarnicola ◽  
Simona Pisanti ◽  
Valeria Tutino ◽  
Domenica Bocale ◽  
Maria Teresa Rotelli ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Fatty Acid ◽  
Olive Oil ◽  
Cancer Cells ◽  
Fatty Acid Synthase ◽  
Human Colorectal Cancer ◽  
Colorectal Cancer Cells ◽  
Human Colorectal Cancer Cells ◽  
Expression And Activity

Assessment of an internal reference gene in Rhodobacter sphaeroides grown under cobalt exposure

2010 ◽  
Vol 50 (3) ◽  
pp. 302-305 ◽  
Author(s):  
Luca Losurdo ◽  
Francesca Italiano ◽  
Massimo Trotta ◽  
Raffaele Gallerani ◽  
Ruggiero Ceci Luigi ◽  
...  
Keyword(s):  
Reference Gene ◽  
Rhodobacter Sphaeroides ◽  
Internal Reference ◽  
Internal Reference Gene ◽  
Cobalt Exposure

Analysis of gene expression in human colorectal cancer tissues by cDNA array

2002 ◽  
Vol 37 (S14) ◽  
pp. 83-86 ◽  
Author(s):  
Hiroyuki Yamamoto ◽  
Arisa Imsumran ◽  
Hiroshi Fukushima ◽  
Yasushi Adachi ◽  
Yongfen Min ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Cdna Array ◽  
Human Colorectal Cancer ◽  
Cancer Tissues

Abstract 823: Gene expression profiling for oncogenic Kras mutation in mice and human colorectal cancer

2015 ◽  
Author(s):  
Hiroaki Niitsu ◽  
Takao Hinoi ◽  
Yasuo Kawaguchi ◽  
Kazuhiro Sentani ◽  
Naohide Oue ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Kras Mutation ◽  
Human Colorectal Cancer

47. Microarray Analysis of Smad4 Gene Expression and Impact On Survival in Human Colorectal Cancer

2008 ◽  
Vol 144 (2) ◽  
pp. 196-197
Author(s):  
J. Joshua Smith ◽  
Bonnie LaFleur ◽  
Kay Washington ◽  
Nipun Merchant ◽  
Robert Coffey ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Microarray Analysis ◽  
Human Colorectal Cancer ◽  
Smad4 Gene ◽  
Impact On Survival
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