scholarly journals The Effect of Treadmill Treatment on Oxidative Stress Markers and Endogenous Antioxidant Status in Obesity Mice

2018 ◽  
Vol 6 (10) ◽  
pp. 1803-1808
Author(s):  
Tiwuk Susantiningsih ◽  
Roro Rukmi Windi Perdani ◽  
Khairunnisa Berawi ◽  
Sutopo Hadi
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Specific Activity ◽  
Oxidative Status ◽  
Laboratory Research ◽  
Oxidative Stress Markers ◽  
Obese Mice ◽  
Stress Markers ◽  
Post Test ◽  
Endogenous Antioxidant

AIM: This study aimed to determine the effect of treadmill treatment on oxidative stress markers and endogenous antioxidant status seen from MDA, GSH, MnSOD enzyme specific activity and blood catalase of obese mice. MATERIALS AND METHODS: This research is experimental laboratory research using post-test control design group only. The study lasted for 28 days and was divided into 4 groups of study, i.e., group K (normal control), KP (obesity control), P1 (obesity mice with 1 x 10-minute treadmill treatment a day), and P2 (obesity mice with 2 x 10-minute treadmill treatment a day). RESULTS: The treadmill treatment had an effect on the improvement of the oxidative status of mice with a decrease of MDA level of obesity mice blood (p ≤ 0.05) compared to KP control. An elevated endogenous antioxidant status of obesity mice was seen from elevated GSH levels, MnSOD specific activity and blood catalase of obesity mice (p ≤ 0.05) compared with KP controls. Treatment of 1 x 10-minute treadmill per day decreased blood MDA level, increased GSH enzyme and increased specific activity of MnSOD enzyme and blood catalase of obese mice. CONCLUSIONS: The 2 x 10-minute daily treadmill did not differ significantly in improving the oxidative status and endogenous antioxidant status compared with the treadmill 1 x 10 minutes a day (p ≥ 0.05).

Increased oxidative stress status in rat serum after five minutes treadmill exercise

Open Medicine ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. 722-728 ◽  
Author(s):  
Florin-Petrut Trofin ◽  
Alin Ciobica ◽  
Dumitru Cojocaru ◽  
Marin Chirazi ◽  
Cezar Honceriu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Status ◽  
Specific Activity ◽  
Total Antioxidant Status ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Oxidative Stress Status ◽  
Total Antioxidant

AbstractAlthough it is accepted that an important correlation exists between the physical exercise and the oxidative stress status, the data regarding the levels of the main oxidative stress markers after physical training have been difficult to interpret and a subject of many controversies. There are also very few studies regarding the effects of short-time exercise on the oxidative stress status modifications. Thus, in the present report we were interested in studying the modifications of some oxidative stress markers (two antioxidant enzymes-superoxide dismutase and glutathione peroxidase, a lipid peroxidation parameter — malondyaldehide, the total antioxidant status and protein carbonyl levels), from the serum of rats that were subject to one bout of five minutes exercise on a treadmill, when compared to a control sedentary group. In this way, we observed a decrease of superoxide dismutase specific activity in the rats which performed the exercises. Still, no modifications of glutathione peroxidase specific activity were found between groups. In addition, increased levels of malondyaldehide and protein carbonyls were observed in the rats subjected to exercises. In conclusion, our data provides new evidence regarding the increase of the oxidative stress status, as a result of a 5-minutes bout of treadmill exercising in rats, expressed through a decrease in the SOD specific activity and the total antioxidant status and also an increase of the lipid peroxidation and protein oxidation processes.

scholarly journals Active recovery training does not affect the antioxidant response to soccer games in elite female players

2010 ◽  
Vol 104 (10) ◽  
pp. 1492-1499 ◽  
Author(s):  
Helena Andersson ◽  
Anette Karlsen ◽  
Rune Blomhoff ◽  
Truls Raastad ◽  
Fawzi Kadi
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Response ◽  
Oxidative Stress Marker ◽  
Oxidative Stress Markers ◽  
Active Recovery ◽  
Dietary Antioxidants ◽  
Antioxidant Defence ◽  
Stress Markers ◽  
Dietary Antioxidant ◽  
Endogenous Antioxidant

Changes in plasma endogenous and dietary antioxidants and oxidative stress markers were studied following two 90 min elite female soccer games separated by 72 h of either active or passive recovery. The active recovery group (n 8) trained for 1 h at 22 and 46 h after the first game (low-intensity cycling and resistance training), while the passive group rested (n 8). Blood samples were taken before the games; immediately after the games; 21, 45 and 69 h after the first game; and immediately after the second game. The oxidative stress markers and antioxidants were not affected by active recovery. The oxidative stress marker GSSG increased by the same extent after both the games, while the lipid peroxidation marker diacron-reactive oxygen metabolite remained unchanged. The endogenous antioxidants total glutathione and uric acid and ferric reducing/antioxidant power increased immediately after both the games with the same amplitude, while increases in cysteine, cysteine–glycine and total thiols reached significant levels only after the second game. The changes in dietary antioxidants after the first game were either rapid and persistent (tocopherols and ascorbic acid (AA) increased; polyphenols decreased) or delayed (carotenoids). This resulted in high pre-second game levels of tocopherols, AA and carotenoids. Polyphenols returned to baseline at 69 h, and were not affected by the second game. In conclusion, the soccer-associated dietary antioxidant defence, but not the endogenous antioxidant defence, is persistent. Similar acute oxidative stress and endogenous antioxidant responses and dissimilar dietary antioxidant reactions occur during two repeated female soccer games. Finally, the complex antioxidant response to soccer is not affected by active recovery training.

scholarly journals Markers of Oxidative Stress and Antioxidant Status in the Plasma, Urine and Saliva of Healthy Mice

2018 ◽  
pp. 921-934 ◽  
Author(s):  
M. GYURÁSZOVÁ ◽  
A. KOVALČÍKOVÁ ◽  
K. JANŠÁKOVÁ ◽  
K. ŠEBEKOVÁ ◽  
P. CELEC ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Blood Collection ◽  
Reference Ranges ◽  
Oxidative Stress Markers ◽  
Male Adult ◽  
Antioxidant Power ◽  
Stress Markers ◽  
Status Assessment

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment. Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.

Oxidative Stress Markers and Antioxidant Status after Oral Iron Supplementation to Very Low Birth Weight Infants

2007 ◽  
Vol 151 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Kristin Braekke ◽  
Anne Grete Bechensteen ◽  
Bente Lise Halvorsen ◽  
Rune Blomhoff ◽  
Kirsti Haaland ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Birth Weight ◽  
Low Birth Weight ◽  
Antioxidant Status ◽  
Iron Supplementation ◽  
Very Low Birth Weight ◽  
Oral Iron ◽  
Oxidative Stress Markers ◽  
Low Birth Weight Infants ◽  
Stress Markers

scholarly journals Moderate Caloric Restriction Partially Improved Oxidative Stress Markers in Obese Humans

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1018
Author(s):  
Dominika Kanikowska ◽  
Alina Kanikowska ◽  
Ewelina Swora-Cwynar ◽  
Marian Grzymisławski ◽  
Maki Sato ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Dietary Intervention ◽  
Real Life ◽  
Ros Generation ◽  
Oxidative Stress Markers ◽  
Short Term ◽  
Anthropometric Parameters ◽  
Stress Markers

Oxidative stress and inflammation are implicated in obesity. Therefore, we investigated whether moderate and short-term calorie restriction (CR) reflects a real-life situation, mediates weight loss, and improves oxidative stress markers. We analyzed oxidative stress markers in patients with obesity undergoing moderate CR. Serum oxidative stress markers (myeloperoxidase (MPO), superoxide dismutase (SOD), catalase, total antioxidant status (TAS), and reactive oxygen species (ROS) (generation by endothelial cells in vitro)) were measured in 53 subjects (mean BMI 37.8 ± 5.9 kg/m2) who underwent 8 weeks of CR, which included a reduction of 300–500 kcal/day. MPO was the most CR-sensitive parameter. The mean level of serum MPO in patients with obesity was 20% higher than that in post CR intervention (p < 0.001). SOD increased by 12% after CR (p < 0.05), which was largely due to the improvement in glucose tolerance and the reduction in insulin resistance after CR. Other tested parameters were not modified during the treatment. CR resulted in an expected decrease in body weight (by 5.9 ± 4.6 kg, p < 0.0001) and other anthropometric parameters. Additionally, it was accompanied by a significant change in hsCRP, hsTNF alpha, hsIL-6, leptin (all p < 0.0001), and HOMA-IR (p < 0.05). Cardiovascular and metabolic parameters were also partially improved. Short-term, moderate CR partially improves antioxidant capacity but is enough to substantially change anthropometric parameters in obese patients. Our observations indicate that mimicking real-life situations and low-cost dietary intervention can be successfully implemented in obesity treatment with a simultaneous moderate effect on antioxidant status.

Cynodon dactylon alleviates radiation-induced behavioral and biochemical changes in the cerebral cortex of mice

2021 ◽  
pp. 2569-2575
Author(s):  
Roopesh Poojary ◽  
Arun Kumar Nayanatara ◽  
Reshma Kumarchandra ◽  
Ganesh Sanjeev
Keyword(s):  
Oxidative Stress ◽  
Cerebral Cortex ◽  
Cognitive Functions ◽  
Antioxidant Status ◽  
Cynodon Dactylon ◽  
Alcoholic Extract ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Radiation Induced

Cynodon dactylon is herb with its rich antioxidant phytoconstituents it has been widely used in traditional medicine. The present study was aimed to investigate the ameliorative role of hydro alcoholic extract of Cynodon dactylon on cerebral cortex in the mice exposed to lethal dose of radiation. Swiss albino mice were treated with 0.25 and 1 g/kg body weight of Cynodon dactylon extract (CDE) via oral gavage for 7 days and subjected to 5 Gy of gamma radiation. All mice were subjected to behavioral and cognitive functions tests. Further, potent markers of radiation including biochemical parameters were evaluated in the cerebral cortex. Mice irradiated with γ- rays displayed a significant decline in the behavior and cognitive functions. Further, decline in antioxidant status and increase in the oxidative stress markers was observed. Pretreatment of hydroalcoholic extract of Cynodon dactylon before exposure to γ-radiation showed significant improvement in the cognitive and behavioral analysis. Further, increase in antioxidant status and a decline in level of oxidative stress markers was observed. The present study confirms the potent protective role of Cynodon dactylon on radiation induced oxidative damage to the cerebral cortex.

Ascorbic acid supplementation does not alter oxidative stress markers in healthy volunteers engaged in a supervised exercise program

2016 ◽  
Vol 41 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Piyawan Bunpo ◽  
Tracy G. Anthony
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Young Adults ◽  
Antioxidant Status ◽  
Exercise Program ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Supervised Exercise ◽  
Significant Change ◽  
Supervised Exercise Program

The purpose of this study was to investigate the impact of ascorbic acid (AA) consumption on the oxidative stress status of untrained volunteers participating in a supervised exercise program. The study included 46 young adults (average age, 23.5 ± 0.59 years; 37 females, 9 males) who remained sedentary (n = 16) or participated in 30 min of outdoor aerobic running (n = 30) at an intensity corresponding to 65%–75% of maximum heart rate for 3 times per week for 12 weeks. Exercised subjects were randomly assigned to an exercise group without AA supplementation (control; n = 10) or received either 250 mg (n = 10) or 500 mg (n = 10) of AA supplementation previous to each exercise session. Blood samples were taken on day 0 and day 84 to evaluate metabolic profiles and antioxidant status. Sedentary subjects underwent in a single bout of aerobic running to determine total antioxidant status (TAS) and malondiadehyde (MDA) at pre- and postexercise with or without AA supplementation. No significant change in TAS was observed. Plasma MDA significantly increased at postexercise (P < 0.05), and AA supplementation decreased MDA level significantly (P < 0.05). After 3 months of exercise, there was no significant change in blood glucose, lipid profile, MDA, TAS, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase activities amongst groups. Supplementation of AA was associated with minor and inconsistent reductions in SOD, GPx, and catalase activities (P < 0.05). These findings indicate that pre-exercise supplementation of ascorbic acid does not alter oxidative stress markers in the plasma and erythrocytes of young adults engaged in a supervised exercise program.

Sign in / Sign up

Export Citation Format

Share Document