scholarly journals The Effect of Strontium Ranelate Gel on Bone Formation in Calvarial Critical Size Defects

2017 ◽  
Vol 5 (7) ◽  
pp. 994-999 ◽  
Author(s):  
Hani El Nahass ◽  
Nada Nour El Din ◽  
Sherine Adel Nasry
Keyword(s):  
Bone Formation ◽  
Strontium Ranelate ◽  
Cost Effective ◽  
Negative Control ◽  
Female Rats ◽  
Control Groups ◽  
Critical Size Defects ◽  
Cost Effective Method ◽  
Significant Difference ◽  
Median Area

AIM: The current study was designed to investigate the effectiveness of locally applied Strontium ranelate to induce bone formation.MATERIALS AND METHODS: Forty-eight female rats were divided into six groups (eight rats in each group): The three test groups included Strontium (SR) 2.5 mg, 5 mg and 10 mg that was dissolved in methylcellulose gel. The control groups included methylcellulose, simvastatin 5 mg and a negative control where the defect was left to heal without any intervention. At 44 days the groups were sacrificed, and the bone defects were assessed histomorphometically to assess bone formation. The data was statistically analysed.RESULTS: There was a statistically significant difference in the amount of new bone formation between all groups, where the 2.5 mg SR group showed the highest median bone percentage, is 41.95 %, followed by the 5, and 10 mg SR demonstrating a median bone are a percentage of 39.89%, and 30.19% respectively. Simvastatin showed a median bone percentage of 36.07 %, while the methylcellulose and the negative control groups demonstrated the lowest median area percentage of 23.12 and 20.70 % respectively.CONCLUSIONS: The study showed that the local application of an SR could up-regulate the bone formation and may prove to be a cost-effective method of bone regeneration.

Survival of Macrobrachium amazonicum embryos submitted to cooling

Zygote ◽  
2017 ◽  
Vol 25 (3) ◽  
pp. 288-295 ◽  
Author(s):  
Arthur Vinícius Lourenço Ferreira ◽  
Moisés Fernandes Martins ◽  
Míriam Luzia Nogueira Martins de Sousa ◽  
Aldeney Andrade Soares Filho ◽  
Célia Maria de Souza Sampaio
Keyword(s):  
Survival Rate ◽  
Storage Time ◽  
Survival Rates ◽  
Negative Control ◽  
Control Groups ◽  
Treatment Groups ◽  
Significant Difference ◽  
Macrobrachium Amazonicum ◽  
Cryoprotectant Solution ◽  
Positive Controls

SummaryCooling techniques have several applications for reproduction in aquaculture. However, few studies have sought to create protocols for cooling and cryopreservation of Macrobrachium amazonicum embryos. Thus, the objective of this work was to verify the survival of M. amazonicum embryos and the correlation between embryonic volume and mortality of M. amazonicum embryos after cooling. Embryo pools were collected from three females and divided into two treatment groups: dimethyl sulfoxide (DMSO) 3% and ethylene glycol (EG) 0.5%, both of them associated with 2 M sucrose. Positive and negative control groups consisted of seawater 10%. Aliquots of 10 µg of embryos were placed in Falcon® tubes containing a cryoprotectant solution and submitted directly to the test temperature of 2°C for 2 and 6 h of cooling. Further analysis of survival and embryonic volume were performed under a stereoscopic microscope. Data were subjected to analysis of variance (ANOVA), and means were compared using the Tukey test at 5%. The highest embryonic survival rate was observed after the shortest storage time for both the DMSO 3% and the 0.5% EG groups, with survival rates of 84.8 ± 3.9 and 79.7 ± 2.8%, respectively. There was a reduction in survival after 24 h, with the DMSO 3% group presenting a survival rate of 71.7 ± 6.6%, and the EG 0.5% group, 66 ± 6.9%. Survival showed a statistically significant difference when compared with the positive controls after 2 h and 24 h of cooling, with 99 ± 0.5% and 95.8 ± 1.5% survival rates, respectively. There was no significant statistical difference in the embryonic volume, but it was possible to observe a change in the appearance of the embryos, from a translucent coloration to an opaque white or brownish coloration, after 24 h in incubators. Thus, it can be concluded that survival is inversely proportional to storage time and that, although there was no change in the embryonic volume after cooling, a change in the appearance of embryos could be observed.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

Comparison of Gravity Collection Versus Suction Collection for Transfusion Purposes in Dogs

2013 ◽  
Vol 49 (5) ◽  
pp. 301-307 ◽  
Author(s):  
Bérénice Conversy ◽  
Marie-Claude Blais ◽  
Lisa Carioto ◽  
Julie Beaudoin
Keyword(s):  
Blood Donors ◽  
Blood Product ◽  
Blood Donation ◽  
Cost Effective ◽  
Clinical Parameters ◽  
Cost Effective Method ◽  
Significant Difference ◽  
Before And After ◽  
Collection Methods ◽  
Time Point

Blood donation is an essential step in transfusion medicine that must take into account the donor’s welfare, collection effectiveness, and blood product quality. This prospective study enrolled 13 canine blood donors, each subjected to both gravity and suction collection methods, in a randomized order. Clinical parameters, including heart rate (HR), respiratory rate (RR), systolic blood pressure (SBP), and rectal temperature (RT), were evaluated at four time points, including when the donor was on the floor and on the collection table, and before and after blood donation. The number of times the donor and needle required repositioning, the duration of the donation, the noise created by the apparatus, and the presence of a hematoma were evaluated. The weight, index of hemolysis, and hematocrit of each unit of blood were recorded. There was no significant difference between collection methods for either the clinical parameters at each time point or the prevalence of hematoma formation, the frequency of needle repositioning, the hemolysis index, or hematocrit. Collection by suction was noisier (P &lt; 0.0001), faster (P = 0.004), and associated with significantly less donor repositioning (P = 0.007). Suction appears to be a safe and cost-effective method that should be considered to optimize blood donation.

scholarly journals Histopathological Profile of Therapeutic Doses of Mango Mistletoe Methanolic Extract (MMME) in Cardiac of Hypertensive Rats (DOCA-Salt)

JSMARTech ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 048-054
Author(s):  
Annisatul Hakimah ◽  
◽  
Nour Sjakoer ◽  
Nurul Mubarakati ◽  
◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Muscle Cells ◽  
Positive Control ◽  
Negative Control ◽  
P Value ◽  
Hypertensive Rats ◽  
Control Groups ◽  
Significant Difference ◽  
Heart Muscle Cells ◽  
And Control

This study determined the effect of Mango mistletoe Methanolic Extract (MMME) on the cardiac's histopathological profile of hypertensive rats (DOCA-Salt) treated. The MMME was treated on fifty rats for 14 days, divided into five treatments: negative control, positive control, doses 50, 100, and 200 mg/kg BW with five replications. One-way ANOVA analysis was used, namely JAMOVI with version 1.1.9.0, and for cell calculation, diameter using the IMAGE J application. Results showed that there were no significant differences in the histopathological profile of the cardiac in hypertensive rats (DOCA-Salt) treated with MMME for 14 days on the diameter of the heart muscle cells between the control (+) and control groups (-), treatments 1, 2, and 3. This is evidenced by the analysis of p-value> 0.05, namely 0.187 millimeters. Therefore, we concluded that MMME does not affect the diameter of heart/cardiac organ muscle cells. However, there was a significant difference in the amount of necrosis in the cardiac of hypertensive rats between control (+) and control (-) groups, treatments 1, 2, and 3. Based on the results, MMME reduces the number of necrosis in the heart/cardiac organ.

scholarly journals Efficacy of titanium brush, 915 nm diode laser, citric acid for eradication of Staphylococcus aureus from implant surfaces

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohammad Reza Karimi ◽  
Behshad Farkhondemehr ◽  
Motahare Ghaeni Najafi ◽  
Ardavan Etemadi ◽  
Nasim Chiniforush
Keyword(s):  
Staphylococcus Aureus ◽  
Citric Acid ◽  
Diode Laser ◽  
Acid Group ◽  
Negative Control ◽  
Control Groups ◽  
Implant Surface ◽  
Mini Implants ◽  
Significant Difference ◽  
Post Hoc

Abstract Background This study aimed to assess the efficacy of titanium brush, 915 nm diode laser, citric acid and the combination of latter two with titanium brush for decontamination of SLA surface mini-implants. Methods Seventy-five mini-implants contaminated with Staphylococcus aureus (S. aureus) were randomly divided into five experimental groups (n = 12) of titanium brush (TiB), laser, citric acid (CA), brush-laser, and brush-acid, positive [n = 12; chlorhexidine mouthwash (CHX)] and negative [n = 2; phosphate buffered saline (PBS)] control groups and one no-treatment group (n = 1). After counting the colony forming units (CFUs), data were analyzed using the Kruskal–Wallis and Dunn post-hoc tests. Results Regardless of the no-treatment and negative control groups, maximum and minimum CFUs were noted in the titanium brush and positive control groups. After CHX, minimum CFUs were noted in brush-acid group followed by brush-laser, laser, and acid groups. Generally, the Kruskal–Wallis test revealed a significant difference between the groups regarding the colony count (P < 0.001). Dunn post-hoc test showed that the difference between the titanium brush and acid-brush group was significant (P < 0.001) while the differences between the brush and laser groups with the brush-laser group were not significant (P > 0.077). Conclusions Combined use of titanium brush and citric acid yielded superior results compared to other groups in reduction of S. aureus on implant surface.

scholarly journals Effect of extracellular matrix and dental pulp stem cells on bone regeneration with 3D printed PLA/HA composite scaffolds

2021 ◽  
Vol 41 ◽  
pp. 204-215
Author(s):  
I Gendviliene ◽  
◽  
E Simoliunas ◽  
M Alksne ◽  
S Dibart ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Negative Control ◽  
Dental Pulp Stem Cells ◽  
Female Rats ◽  
Micro Computed Tomography ◽  
Significant Difference ◽  
Gender Specific Differences

The demand for bone grafting procedures in various fields of medicine is increasing. Existing substitutes in clinical practice do not meet all the criteria required for an ideal bone scaffold, so new materials are being sought. This study evaluated bone regeneration using a critical-size Wistar rat’s calvarial defect model. 12 male and 12 female rats were evenly divided into 3 groups: 1. Negative and positive (Geistlich Bio-Oss®) controls; 2. polylactic acid (PLA) and PLA/hydroxyapatite (HA); 3. PLA/HA cellularised with dental pulp stem cells (DPSC) and PLA/HA extracellular matrix (ECM) scaffolds. PLA/HA filament was created using hot-melt extrusion equipment. All scaffolds were fabricated using a 3D printer. DPSC were isolated from the incisors of adult Wistar rats. The defects were evaluated by micro-computed tomography (µCT) and histology, 8 weeks after surgery. µCT revealed that the Bio-Oss group generated 1.49 mm3 and PLA/HA ECM 1.495 mm3 more bone volume than the negative control. Histology showed a statistically significant difference between negative control and both (Bio-Oss and PLA/HA ECM) groups in rats of both genders. Moreover, histology showed gender-specific differences in all experimental groups and a statistically significant difference between cellularised PLA/HA and PLA/HA ECM groups in female rats. Qualitative histology showed the pronounced inflammation reaction during biodegradation in the PLA group. In conclusion, the bone-forming ability was comparable between the Bio-Oss and PLA/HA ECM scaffolds. Further research is needed to analyse the effects of ECM and PLA/HA ratio on osteoregeneration.

scholarly journals Hand Hygiene Practices among Medical Students

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Azzam al Kadi ◽  
Sajad Ahmad Salati
Keyword(s):  
Medical Students ◽  
Hand Hygiene ◽  
Cost Effective ◽  
World Health ◽  
Undergraduate Medical Students ◽  
Clinical Phase ◽  
Healthcare Settings ◽  
Hygiene Practices ◽  
Cost Effective Method ◽  
Significant Difference

Background. Hand hygiene is a cost-effective method in preventing infection transmission. Hand hygiene practices have been found to be faulty in most healthcare settings. We conducted a study to evaluate the awareness, and compliance of hand hygiene among undergraduate medical students during their clinical phase in Qassim College of Medicine, Saudi Arabia.Methods. A questionnaire based on World Health Organization’s concept of “Five Moments for Hand Hygiene” was used to evaluate the awareness of the indications for hand hygiene and compliance was observed during Objective Structured Clinical Examination (OSCE) sessions. Sixty students including thirty-six males (60%) and twenty-four females (40%) participated voluntarily in the study.Results. The average awareness regarding the positive indications of hand hygiene was 56%. Rest of the 44% of students were either not sure or unaware of the indications of hygiene. Only 29% of students were able to identify all the five indications for hand hygiene in the questionnaire. Compliance as assessed during OSCE sessions was only 17% with no significant difference between the genders.Conclusion. It was concluded that serious efforts are needed to improve the hand hygiene practices among medical students.

scholarly journals Ultrasound microbubble-mediated CRISPR/Cas9 knockout of C-erbB-2 in HEC-1A cells

2019 ◽  
Vol 47 (5) ◽  
pp. 2199-2206 ◽  
Author(s):  
Junhong Cai ◽  
Sizhe Huang ◽  
Yuping Yi ◽  
Shan Bao
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Western Blotting ◽  
Growth Factor Receptor ◽  
Negative Control ◽  
Rt Pcr ◽  
Control Groups ◽  
Transfected Cells ◽  
Significant Difference ◽  
Ultrasound Microbubbles

Objective Epidermal growth factor receptor 2 (C-erbB-2) is one of the most frequently mutated oncogenes in human tumors. We aimed to evaluate the knockout efficiency of clustered regularly interspaced short palindromic repeat (CRISPR) technology using ultrasound microbubble transfection to target C-erbB-2 in human endometrial cancer (HEC)-1A cells. Methods Three single guide RNAs (sgRNAs) targeting C-erbB-2 were designed and used to construct CRISPR/CRISPR-associated (Cas)9-C-erbB-2 plasmids. The constructed plasmids were transfected into HEC-1A cells using ultrasound microbubbles. C-erbB-2 knockout cloned cells were identified by green fluorescence. C-erbB-2 mRNA and protein expression was measured by reverse transcription (RT)-PCR and western blotting, respectively. Results RT-PCR showed that C-erbB-2 mRNA expression was significantly lower in sgRNA1-transfected cells (0.57 ± 0.06) than in blank (1.00 ± 0.09) and negative-control groups (1.02 ± 0.12). Western blotting revealed C-erbB-2 protein expression to be significantly lower in sgRNA1-transfected cells (0.269 ± 0.033) than in blank (0.495 ± 0.059) and negative-control groups (1.243 ± 0.281). However, there was no significant difference in C-erbB-2 protein and mRNA expression in sgRNA2- and sgRNA3-transfected cells compared with controls. Conclusion Ultrasound microbubbles can mediate plasmid transfer into HEC-1A cells to interfere with gene expression and knockout C-erbB-2.

scholarly journals Bio Fertilizer on Rachis: A New Method Facilitates Higher Banana (Musa sapientum) Production

2019 ◽  
Author(s):  
Abul Khayer ◽  
Fatiha Sultana Eti ◽  
Arif Istiaq ◽  
Md. Mohibul Hasan
Keyword(s):  
Test Group ◽  
Male Flower ◽  
Cost Effective ◽  
Fertilizer Application ◽  
Positive Control ◽  
Negative Control ◽  
New Method ◽  
Cow Dung ◽  
Control Groups ◽  
Banana Production

Background and Objectives: Due to huge demand and availability of Banana, innovative cost effective method is necessary to promote and smoothen the banana production among farmers commercially mitigating the demand. Method and Materials: In this study, we feed cow dung mixture along with Urea, TSP, MoP, water to the distal part of rachis after cutting down male bud as soon as the female flowers matured into fruits (T1). The effect of this method was then compared with two control groups; one with the same strategy except fertilizer applied on root following ring method (C1, Positive control) and another was male flower untouched without applying fertilizer on rachis or root (C2, Negative control). Results and Conclusion: T1 showed more than double increase in length than controls. In the same way, in case of shape (diameter), T1 (0.46 cm) showed twice as better growth in the C1 (0.22 cm) and C2 (0.18 cm). Trend analysis showed the test group T1 curve is much steeper than the control groups suggesting faster growth rate than the other two. Finally, the cost of fertilizers for T1 per plant was estimated 0.091 USD while for positive control C1 it was 2.9 USD. This study shows an approach to be effective and economic comparing to traditional method of fertilizer application, which can be adapted as a new method of banana production.

THE EFFECT OF hPRP AND AUTOGRAFT-PRP ON BONE HEALING IN THE RADIAL DEFECT OF RAT

2016 ◽  
Vol 19 (01) ◽  
pp. 1650003
Author(s):  
Ahmad Oryan ◽  
Amin Bigham-Sadegh ◽  
Somayeh Monazzah
Keyword(s):  
Bone Formation ◽  
Cortical Bone ◽  
Bone Healing ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Negative Control ◽  
Post Injury ◽  
Non Union ◽  
Difference Formula ◽  
Significant Difference

Objective: The present study was designed to investigate the effects of platelet rich plasma (PRP) and autograft-human PRP on bone healing in a rat model. Methods: A critical sized defect at least twice as long as the diameter of the diaphysis was made in 16 rats to create a non-union model. The defect was either supplied with hPRP, or autograft-hPRP (experimental groups) and autograft (positive control) or left empty (negative control). Radiographs of each forelimb was taken postoperatively on the 1st day and then at the 35th, and 56th days post-injury to evaluate bone formation, union and remodeling of the defect. The operated radiuses were removed on 56th post-operative day and were evaluated biomechanically, histopathologically and ultrastructurally by scanning electron microscopy. Results: There was significant difference ([Formula: see text]0.05) between the groups in union and cancellous bone so that the autograft, autograft-hPRP and hPRP groups were significantly ([Formula: see text]0.05) superior to the empty group and in cortical bone formation the autograft-PRP group was significantly ([Formula: see text]0.05) superior to the other three groups but there was no significant difference ([Formula: see text]0.05) between the groups in cortical bone marrow. Biomechanical evaluation did not show any significant differences. There was no significant difference ([Formula: see text]0.05) between the groups in radiological parameters at 35th and 56th post-operative days. Conclusion: This study demonstrated that autograft-hPRP is most effective and could promote bone regeneration in the critical sized defects in rat model.

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