scholarly journals The Use of Different Irrigation Techniques to Decrease Bacterial Loads in Healthy and Diabetic Patients with Asymptomatic Apical Periodontitis

2016 ◽  
Vol 4 (4) ◽  
pp. 714-719 ◽  
Author(s):  
Mai Ghoneim ◽  
Shehab ElDin Saber ◽  
Tarek El-Badry ◽  
Maram Obeid ◽  
Nehal Hassib
Keyword(s):  
Irrigation System ◽  
Apical Periodontitis ◽  
Diabetic Patients ◽  
Bacterial Reduction ◽  
Multisystem Disease ◽  
Root Canals ◽  
Promising Tool ◽  
Before And After ◽  
Root Canal System ◽  
Reducing Bacteria

BACKGROUND: Diabetes mellitus is a multisystem disease which weakens the human’s immunity. Subsequently, it worsens the sequelae of apical periodontitis by raising a fierce bacterial trait due to the impaired host response.AIM: The study aimed to estimate bacterial reduction after using different irrigation techniques in systemically healthy and diabetic patients with asymptomatic apical periodontitis.MATERIAL AND METHODS: Enterococcus faecalis, Peptostreptococcus micros, and Fusobacterium necleatum bacteria were chosen, as they are the most common and prevailing strains found in periodontitis. Bacterial samples were retrieved from necrotic root canals of systemically healthy and diabetic patients, before and after endodontic cleaning and shaping by using two different irrigation techniques; the conventional one and the EndoVac system. Quantitive polymerase chain reaction (qPCR) was utilised to detect the reduction in the bacterial count.RESULTS: The EndoVac irrigation system was effective in reducing bacteria, especially Peptostreptococcus micros in the diabetic group when compared to conventional irrigation technique with a statistically significant difference.CONCLUSION: The EndoVac can be considered as a promising tool in combination with irrigant solution to defeat the bacterial colonies living in the root canal system. Additional studies ought to be done to improve the means of bacterial clearance mainly in immune-compromised individuals.

scholarly journals Antibacterial Efficacy of Synthetic and Natural-Derived Novel Endodontic Irrigant Solutions

2018 ◽  
Vol 29 (5) ◽  
pp. 459-464 ◽  
Author(s):  
Larissa Tais Soligo ◽  
Ediléia Lodi ◽  
Ana Paula Farina ◽  
Matheus Albino Souza ◽  
Cristina de Mattos Pimenta Vidal ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Enterococcus Faecalis ◽  
Grape Seed ◽  
Control Group ◽  
Bacterial Reduction ◽  
Calcium Hypochlorite ◽  
Root Canals ◽  
Colony Forming Units ◽  
Before And After ◽  
Post Hoc

Abstract The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO)2) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO)2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.

scholarly journals A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin

2021 ◽  
Vol 11 (21) ◽  
pp. 10315
Author(s):  
Nang Noon Shean Aye ◽  
Pornsuda Maraming ◽  
Ratree Tavichakorntrakool ◽  
Attawut Chaibunruang ◽  
Patcharee Boonsiri ◽  
...  
Keyword(s):  
Glycated Albumin ◽  
Diabetic Patients ◽  
Label Free ◽  
Serum Samples ◽  
Covalent Interaction ◽  
Promising Tool ◽  
Before And After ◽  
Carboxylic Groups ◽  
Free Graphene ◽  
Specific Proteins

Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependent on the covalent interaction of the amine-functionalized GA-specific aptamer with the carboxylic groups of GO. Square wave voltammetry (SWV) analysis was carried out for the measurement of GA-aptamer binding to their specific proteins. The peak current changes before and after incubation with GA protein were directly proportional to the concentration. The developed aptasensor exhibited a broad linearity (1–10,000 µg mL−1), a low detection limit (LOD) of 0.031 µg mL−1, and high selectivity for GA detection. In addition, the aptasensor was successfully applied to detect GA in both spiked and clinical serum samples. The comparison of the developed method with a commercial assay validated the reliability of the aptasensor for clinical application. Therefore, the newly developed aptasensor is a promising tool for GA measurements in diabetic patients with underlying thalassemia.

scholarly journals Bacterial removal in flattened root canals with different tapers of ProDesign Logic single-file system

2021 ◽  
Vol 10 (9) ◽  
pp. e5910917701
Author(s):  
Vanessa Sandini ◽  
Viviane Godoy ◽  
Marina Carvalho Prado ◽  
Ricardo Ferreira ◽  
Adriana De-Jesus-Soares ◽  
...  
Keyword(s):  
Bacterial Biofilm ◽  
Bacterial Reduction ◽  
Sterile Saline ◽  
Root Canals ◽  
Bacterial Removal ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Before And After ◽  
Level Of Significance ◽  
Post Hoc

Objective: This study evaluated the bacterial removal in flattened root canals after preparation with different tapers of ProDesign Logic instruments. Methodology: Fifty-five mandibular incisors with flattened root canals were contaminated with a pure culture of Enterococcus faecalis ATCC 29212 for 21 days at 37ºC. Root canals preparation was performed using ProDesign Logic and 0.9% sterile saline solution for irrigation. The specimens were randomly allocated to three experimental groups (n = 15) according to the taper of the instrument used: 25.03, 25.04, or 25.06. Intracanal bacteriological samples were taken before (S1) and after (S2) preparation and the number of colony-forming units per milliliter (CFU/mL) was calculated. Differences in bacterial counts before and after treatment, within each group, were analyzed by the paired t-test. Groups were compared by ANOVA with a post hoc test. The level of significance was set at p <0.05. Results: Mature biofilm formation was confirmed at root canal walls by scanning electron microscopy. Bacterial colonies were significantly reduced in all experimental groups at S2 compared to S1 (p <0.001). No significant difference was observed between groups regarding the percentage of bacterial reduction (p >0.05). Conclusion: Preparation of flattened root canals using ProDesign Logic instruments with tapers of 0.03, 0.04 and 0.06 resulted in similar planktonic and bacterial biofilm reduction. None of the instruments rendered root canals completely free of bacteria.

scholarly journals Disinfection and outcome of root canal treatment using single-file or multifile systems and Ca(OH)2 medication

2020 ◽  
Vol 31 (5) ◽  
pp. 493-498
Author(s):  
Mônica A. S. Neves ◽  
José C. Provenzano ◽  
Simone C. L. Fonseca ◽  
Renata C. V. Rodrigues ◽  
Lucio S. Gonçalves ◽  
...  
Keyword(s):  
Success Rate ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Apical Periodontitis ◽  
Antibacterial Effects ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Single File ◽  
Polymerase Chain ◽  
Clinical Study Reports

Abstract This clinical study reports on the antibacterial effects and outcome of endodontic treatment using either a single-file or a multifile system, associated with calcium hydroxide interappointment medication. The root canals of single-rooted teeth with apical periodontitis were treated by using either Reciproc or BioRaCe instrument systems, 2.5% NaOCl irrigation, and calcium hydroxide medication. Bacteriological samples taken before preparation and immediately before obturation were evaluated for total bacterial counts by quantitative real-time polymerase chain reaction (qPCR). Patients were followed up and the treatment outcome was assessed by clinical and radiographic criteria. Decreasing lesions were classified as success in a lenient criterion or failure in a rigid one. Bacteria were detected in all initial samples (47 cases) and were significantly reduced after treatment in both groups (p<0.001). In the Reciproc and BioRaCe groups, 7/25 (28%) and 11/22 (50%) root canals yielded negative qPCR results before obturation, respectively (p>0.05). Quantitative bacterial reduction was similar between groups (p>0.05). The success rate in the BioRaCe group was 95.5% and 77% in the loose and rigid criterion, respectively. In the Reciproc group, corresponding figures were 88% and 76%. Differences in outcome were not significant (p>0.05). No diseased case showed negative qPCR results for bacteria. A difference of >1 Log10 counts was observed between healed and diseased cases. Root canal treatments of teeth with apical periodontitis using a single-file or a multifile system for preparation, associated with NaOCl irrigation and calcium hydroxide interappointment medication, showed similar antibacterial effectiveness and success rate.

scholarly journals OPTIMIZATION OF TREATMENT OF DESTRUCTIVE FORMS OF CHRONIC APICAL PERIODONTITIS

2017 ◽  
Vol 21 (2) ◽  
pp. 85-89 ◽  
Author(s):  
O. A Pavlovich ◽  
L. A Skorikova ◽  
N. P Bazhenova ◽  
N. V Lapina
Keyword(s):  
Root Canal ◽  
Antibacterial Effect ◽  
Apical Periodontitis ◽  
Breeding Ground ◽  
Canal System ◽  
Root Canals ◽  
Comparison Groups ◽  
Root Canal System ◽  
A Priority ◽  
Positive Results

Increase of efficiency of treatment of patients with chronic apical periodontitis is a priority in dentistry. A root canal is a breeding ground for microorganisms. The main reason for unfavourable treatment outcomes of teeth with chronic apical periodontitis is incomplete debridement of the root canal system. Optimizing the treatment of patients with chronic periodontitis with the use of complex drugs galodent No. 4 (clotrimazole, tracert and calcium hydroxide) is a priority in dentistry. As a result of researches it is established that the antibacterial effect of the temporary investment of complex drugs galodent No. 4 and clotrimazole in the root canals of teeth with chronic apical periodontitis is 2,9 times higher and the proportion of positive results is, on average, 1,5 times higher compared to the practiced methods in the comparison groups

Short-chain Fatty Acids in Infected Root Canals of Teeth with Apical Periodontitis before and after Treatment

2015 ◽  
Vol 41 (6) ◽  
pp. 831-835 ◽  
Author(s):  
José Claudio Provenzano ◽  
Isabela N. Rôças ◽  
Luís Fernando D. Tavares ◽  
Bianca Cruz Neves ◽  
José F. Siqueira
Keyword(s):  
Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Apical Periodontitis ◽  
Short Chain ◽  
Root Canals ◽  
Infected Root ◽  
Before And After ◽  
Chain Fatty Acids

Аpplying of high-frequency monopolar diathermocoagulation in the treatment of chronic pulpitis

2020 ◽  
Vol 1 (12) ◽  
pp. 40-42
Author(s):  
F. Yu. Daurova ◽  
D. I. Tomaeva ◽  
S. V. Podkopaeva ◽  
Yu. A. Taptun
Keyword(s):  
Root Canal ◽  
High Frequency ◽  
Antibacterial Effect ◽  
Comparison Group ◽  
Poor Quality ◽  
Endodontic Treatment ◽  
Root Canals ◽  
The Third ◽  
Before And After

Relevance: the reason for the development of complications in endodontic treatment is poor-quality instrumental treatment root canals.Aims: a study of the animicrobial action and clinical efficacy of high-frequency monopolar diathermocoagulation in the treatment of chronic forms of pulpitis.Materials and methods: 102 patients with various chronic forms of pulpitis were divided into three groups of 34 patients each. In the first two groups, high-frequency monopolar diathermocoagulation was used in endodontic treatment in different modes. In the third group, endodontic treatment was carried out without the use of diathermocoagulation (comparison group). The root canal microflora in chronic pulpitis in vivo was studied twice-before and after diathermocoagulation.Results: it was established that high-frequency monopolar diathermocoagulation in the effect mode is 3, power is 4 (4.1 W) and effect is 4, power is 4 (5.4 W) with an exposure time of 3 seconds, it has a pronounced antibacterial effect on all presented pathogenic microflora obtained from the root canals of the teeth.

scholarly journals A preliminary study on diabetes self-management education and glycemic control among patients with diabetes mellitus

2019 ◽  
Vol 9 (9) ◽  
pp. 98 ◽  
Author(s):  
Kisokanth G. ◽  
Indrakumar J. ◽  
Prathapan S. ◽  
Joseph J. ◽  
Ilankoon I.M.P.S.
Keyword(s):  
Diabetes Mellitus ◽  
Glycemic Control ◽  
Management Education ◽  
Self Management ◽  
Rank Test ◽  
Diabetic Patients ◽  
Effective Measure ◽  
Before And After ◽  
Patients With Diabetes ◽  
Preliminary Study

This study was aimed to assess the effectiveness of diabetes self-management education (DSME) in the improvement of glycemic control among patients with type 2 Diabetes Mellitus (T2DM) in Batticaloa District, Sri Lanka. The study was a prospective interventional study and conducted as a preliminary study at medical clinic, Base hospital, Kaluwanchikudy, Batticaloa. Thirty patients with T2DM were included based on inclusion and exclusion criteria. A structured individual diabetes self-management education for 10 hours (one hour per week) was delivered to diabetic patients by the trained Nurse Health Educator. Glycosylate hemoglobin (HbA1c) was assessed as a main outcome measure and Fasting Blood Sugar (FBS), Body Mass Index (BMI) of each patient were also measured and recorded before and after the intervention. The respondent rate was 96.7% (n = 29). Majority of them were females (n = 25, 86.2%). A Wilcoxon signed rank test showed that DSME had a statistically significant reduction in HbA1c [8.60 (IQR 2.60) vs. 7.40 (IQR 2.10), p = .000] and FBS level [159.00 (IQR 77.50) vs. 134.00 (IQR 40.50), p = .002] at 3 months of intervention. The mean BMI at baseline was higher compared to 3 months of intervention [24.88 (SD ± 3.06) vs. 24.19 (SD ± 2.79)] which was statistically significant (p = .000). Majority of participants (n = 22, 75.9%) had improved their HbA1c level by ≥ 0.5% in 3 months. The diabetes self-management education is an effective measure in improving glycemic control and other clinical parameters among patients with T2DM. Thus, DSME needs to be implemented among clinic patients with T2DM for the better outcome and the preventions of complications.

scholarly journals Micro-tomographic characterization of the root and canal system morphology of mandibular first premolars in a Chilean population

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alfredo Sierra-Cristancho ◽  
Luis González-Osuna ◽  
Daniela Poblete ◽  
Emilio A. Cafferata ◽  
Paola Carvajal ◽  
...  
Keyword(s):  
Root Canal ◽  
Multiple Root ◽  
Root Anatomy ◽  
Type I ◽  
Micro Computed Tomography ◽  
Canal System ◽  
Root Canals ◽  
Wide Range ◽  
Root Canal System ◽  
Chilean Population

AbstractThis study aimed to analyze the root anatomy and root canal system morphology of mandibular first premolars in a Chilean population. 186 teeth were scanned using micro-computed tomography and reconstructed three-dimensionally. The root canal system morphology was classified using both Vertucci’s and Ahmed’s criteria. The radicular grooves were categorized using the ASUDAS system, and the presence of Tomes’ anomalous root was associated with Ahmed’s score. A single root canal was identified in 65.05% of teeth, being configuration type I according to Vertucci’s criteria and code 1MP1 according to Ahmed’s criteria. Radicular grooves were observed in 39.25% of teeth. The ASUDAS scores for radicular grooves were 60.75%, 13.98%, 12.36%, 10.22%, 2.15%, and 0.54%, from grade 0 to grade 5, respectively. The presence of Tomes’ anomalous root was identified only in teeth with multiple root canals, and it was more frequently associated with code 1MP1–2 of Ahmed’s criteria. The root canal system morphology of mandibular first premolars showed a wide range of anatomical variations in the Chilean population. Teeth with multiple root canals had a higher incidence of radicular grooves, which were closely related to more complex internal anatomy. Only teeth with multiple root canals presented Tomes’ anomalous root.

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