scholarly journals Total IgE Distribution in Food Allergy Suspected Patients in Republic of Macedonia (2001-2011)

2015 ◽  
Vol 3 (2) ◽  
pp. 202-208
Author(s):  
Slavica Hristomanova Mitkovska ◽  
Dejan Trajkov ◽  
Aleksandar Petlichkovski ◽  
Olivija Efinska-Mladenovska ◽  
Mirko Spiroski
Keyword(s):  
Food Allergy ◽  
Human Genetics ◽  
Specific Ige ◽  
Average Concentration ◽  
Total Serum ◽  
Age Group ◽  
Total Ige ◽  
Serum Ige ◽  
Number Of Patients

BACKGROUND: IgE may be considered the hallmark of allergic disorders. It is easily detected in serum and can be measured as total IgE and as allergen-specific IgE. In fact, the serum IgE assay is used to diagnose an allergy.AIM: The aim of this study is to evaluate, investigate and present the distribution of total serum IgE levels, determined with UniCap system, in food-allergy suspected patients in a Republic of Macedonia.MATERIAL AND METHODS: In this study we analyzed retrospectively 8898 consecutive patients that were admitted for allergy testing at the Institute of Immunobiology and Human Genetics during the ten year period between 01.01.2001 and 01.01.2011. Total IgE levels in patient sera were detected with the in vitro system UniCAP100 (Pharmacia, Uppsala, Sweden).RESULTS: When we analyzed the number of patients according to the total IgE groups, we noted that most of the patients have normal levels of total IgE in serum. However, we also discovered a group of patients with elevated levels of total IgE that are greater than 200 kU/L. The average concentration of total serum IgE is higher in women in the age group 6 (6-7 years), followed by a steep decrease in the age group 9 (9-10 years), and after that the average concentrations of total IgE were mostly constant with the exception of a partial increase in the age group 21 (65-69 years). For men, the average serum concentrations of total IgE were highest in the age group of 6 (6-7 years), which was significantly higher than the average concentrations of total IgE in all other age groups.CONCLUSION: The large number of enrolled patients, a particular strength of this study, revealed that average concentrations of total IgE in men are higher than in women and that total IgE did not decrease with age. On the contrary, increased total IgE levels were found in patients aged 65 and 69 of both genders. We continue our work with analyses of the specific IgE antibodies values toward food and the correlation with total IgE values.

scholarly journals Relationship among total tear IgE, specific serum IgE, and total serum IgE levels in patients with pollen-induced allergic conjunctivitis

2021 ◽  
Author(s):  
Yasuo Yamana ◽  
Satoshi Yamana ◽  
Eiichi Uchio
Keyword(s):  
Allergic Conjunctivitis ◽  
Reference Value ◽  
Japanese Cedar ◽  
Specific Ige ◽  
Total Serum ◽  
Serum Ige ◽  
Specific Serum ◽  
Number Of Patients ◽  
Significant Difference ◽  
Japanese Cedar Pollinosis

Abstract Background Recently, the number of patients with pollinosis, particularly Japanese cedar pollinosis, has markedly increased. We previously reported about local allergic conjunctivitis, which is a phenotype of allergic conjunctivitis (AC). AC cases are often sensitized by various antigens. This study aimed to investigate the relationship among total tear IgE (t-tIgE), specific serum IgE (s-sIgE), and total serum IgE (t-sIgE) levels in patients with pollen-induced AC. Methods In 2019, 1372 patients were clinically diagnosed with AC at the Yamana Eye Clinic using t-tIgE, t-sIgE, and s-sIgE tests against 39 allergens. Among the pollen-induced AC patients who underwent allergen testing, 99 tested positives for s-sIgE against pollen. The subjects comprised 33 (33.3%) male and 66 (66.7%) female individuals aged 9–86 years. Results The t-tIgE test was positive in 68 (68.7%) patients and negative in 31 (31.3%) patients. In the t-sIgE test, 45 (45.5%) patients had t-sIgE levels above the reference value of 170 IU/mL. The higher the total score of the positive class value of each pollen-specific IgE (pollen-sIgE) antibody, the higher the positive rate of t-tIgE (p < 0.001). Of 32 patients in whom food-specific IgE (food-sIgE) was detected, 81.3% of the pollen-sIgE-positive and food-sIgE-positive cases were also positive for t-sIgE and t-tIgE. However, significant difference was not found between the total score of food-sIgE of the t-tIgE positive group and negative group. Conclusions Pollen-induced AC is caused by pollen sensitization of the conjunctiva. Food-induced AC might be induced by the different pathological mechanism involved in pollen-induced AC.

Alatop: A New In Vitro Screening Test for Atopy

1994 ◽  
Vol 22 (6) ◽  
pp. 313-322
Author(s):  
M Yazicioǧlu ◽  
P Sayinbaş ◽  
Ü Öneş ◽  
A Saltik ◽  
M Tug˜rul
Keyword(s):  
Antibody Test ◽  
Atopic Disease ◽  
Specific Ige ◽  
Total Serum ◽  
Healthy Children ◽  
Serum Ige ◽  
Prick Test ◽  
Skin Prick Tests ◽  
Inhalant Allergens

The value of a new in vitro multiallergen IgE antibody test in the diagnosis of atopy in children was compared with that of skin-prick tests and total serum IgE level determination. Twenty children with clinical histories and symptoms of asthma, median age 5 years, and 20 healthy children, median age 5.5 years, were enrolled. Total serum IgE levels were evaluated as either normal or high by referring to kit values. Specific IgE antibodies to 12 different inhalant allergens were screened by the Alatop test. The accuracy figures for the tests compared with clinical diagnosis were 65.0%, 72.5% and 80.0%, for the total serum IgE determination, the Alatop test and the skin-prick test, respectively, and the other measures of clinical reliability showed a similar pattern. Although the skin-prick tests were the most sensitive and specific of the three, for screening atopic disease in children, the Alatop test provides a valuable alternative, and the combined use of skin-prick tests with the Alatop test will provide a more reliable screen than using any single test.

scholarly journals A clinical study of inhalant allergens in patients with allergic rhinitis

2017 ◽  
Vol 3 (2) ◽  
pp. 380
Author(s):  
Suhas Y. Choudhari ◽  
Aravind B. Sangavi
Keyword(s):  
Allergic Rhinitis ◽  
Specific Ige ◽  
Total Serum ◽  
In Vitro Test ◽  
Vitro Assay ◽  
Serum Ige ◽  
Dust Mite ◽  
Inhalant Allergens ◽  
High Degree

<p class="abstract"><strong>Background:</strong> The basis of diagnosis of allergy requires a good history and examination, however, the diagnosis cannot be confirmed on the basis of symptoms alone, because both allergic and non-allergic conditions can present with similar symptoms. Hence, allergy testing in the form of specific IgE (sIgE) measurement is an important aid in demonstrating both the presence and severity of such an allergy. The present study was undertaken to find out the common environmental allergens prevailing in Raichur causing allergic rhinitis, using carbohydrate cross reactive determinants (CCD), an in vitro test with high degree of sensitivity.</p><p class="abstract"><strong>Methods:</strong> The present prospective study was conducted among 30 patients with allergic rhinitis. A detailed general andENT examination were done, X-ray ofPNS, CT scan ofPNS, diagnostic nasal endoscopy and nasal smear examination for eosinophils, absolute eosinophil count and serum IgE levels using  Euroimmun system of in vitro assay of specific IgE antibodies.  </p><p class="abstract"><strong>Results:</strong> Allergy to dust mite, D. faranie, corn, carnation flower, sunflower, sheep wool and straw dust were the most frequent allergens causing allergic symptoms among patients in Raichur area.  Total serum IgE was elevated in all the patients, 60% were allergic to dust mite as found by anti CCD specific IgE.</p><p><strong>Conclusions:</strong> Antibodies to dust mite D. faranie, rye, T. mothy grass was the commonest finding. Identification of inhalant allergens is an important factor in prevention and treatment of allergic rhinitis. </p>

A Descriptive Study on Serum IgE and Absolute Eosinophil Count in Children with Bronchial Asthma Attending the Paediatric Department of a Medical College in Central Kerala

2021 ◽  
Vol 8 (33) ◽  
pp. 3097-3103
Author(s):  
Sajna Gamaly Leela ◽  
Menon Narayanankutty Sunilkumar ◽  
Ramaraj Subbiah
Keyword(s):  
Bronchial Asthma ◽  
Eosinophil Count ◽  
Immunoglobulin E ◽  
Asthma Severity ◽  
Health Concern ◽  
Age Group ◽  
Total Ige ◽  
Serum Ige ◽  
Absolute Eosinophil Count ◽  
Paediatric Department

BACKGROUND Bronchial asthma is a major public health concern especially in the paediatric population. An elevated immunoglobulin E (IgE) level is considered as an objective marker of allergy. Because IgE is a mediator of allergic response, quantitative measurement of IgE together with other clinical markers, can provide useful information for the differentiation between atopic and non-atopic diseases. In this study, we wanted to determine serum IgE and absolute eosinophil count (AEC) levels and correlate both levels with severity of asthma in children in the age group of 2 - 15 years admitted in the paediatric department. METHODS This is a descriptive cross-sectional study. After taking the informed consent from the parent or guardian, relevant information on asthmatic symptoms and severity, other associated manifestations, precipitating factors, and family history of asthma were recorded in a pre-designed proforma. A total of 65 children (age group 2 – 15 years old) with bronchial asthma were studied. Investigations such as hemogram and serum IgE levels were determined. Asthma severity was assessed according to global initiative for asthma (GINA) guidelines. RESULTS Among 65 children, 69.2 % were boys. Receiver operating characteristic curve (ROC) analysis showed that total IgE cut-off concentration of 168 IU/ml, distinguishes children with intermittent asthma from those with mild persistent disease. IgE cut-off concentration of 989 IU/ml distinguishes mild persistent from those with moderate persistent asthma. Both showed adequate or good diagnostic efficacy. Cut off value of 168 IU/ml may prove useful in practice, indicating that 75 % of children in intermittent group will have serum concentration of total IgE < 168 IU/ml. No association was observed between AEC and increasing severity of asthma. Only atopic dermatitis with asthma showed increasing trend of IgE levels. CONCLUSIONS Asthmatic children with higher asthma severity have a higher serum total IgE concentration (168 IU/ml and 989 IU/ml are the cut off values for differentiating intermittent from mild persistent and mild persistent from moderate persistent). KEYWORDS Serum IgE, Absolute Eosinophil Count, Childhood Asthma, Asthma Severity

scholarly journals Domestic Exposure to Fungi and Total Serum IgE Levels in Asthmatic Children

2005 ◽  
Vol 2005 (3) ◽  
pp. 167-170 ◽  
Author(s):  
Huey-Jen Jenny Su ◽  
Pei-Chih Wu ◽  
Huan-Yao Lei ◽  
Jiu-Yao Wang
Keyword(s):  
Airborne Fungi ◽  
Total Serum ◽  
Dose Response Relationship ◽  
Total Ige ◽  
Serum Ige ◽  
Asthmatic Children ◽  
Specific Serum ◽  
Dust Mite ◽  
Der P1 ◽  
Dust Mite Allergen

We measured the number of airborne, viable fungi and house dust mite (HDM) allergen levels in the homes of a group of asthmatic children. Blood samples were drawn and the amounts of total and specific serum IgE were determined. The association between the number of fungal colonies, dust mite allergen exposure, and specific and total IgE was evaluated. The number of viable airborne fungi was high (20 543 CFU/m) in those investigated houses.Der p1concentrations on child's mattress exceeding 2μg/g were found in 78.6% of the houses. A quantitative dose-response relationship was demonstrated between the exposure to viable, airborne molds and the amount of total IgE (r=0.4399andP=.0249) and the level was further increased in children with coexposure to viable fungi and HDM.

A Comparison of Acti*Tip™ Allerg*E™ and Allerg*ENS™ System to Phadebas RAST® and Phadebas IgE PRIST® in Assaying Total IgE and Specific IgE in Human Serum

1988 ◽  
Vol 2 (3) ◽  
pp. 135-140 ◽  
Author(s):  
John T. Connell
Keyword(s):  
Solid Phase ◽  
Detection System ◽  
Specific Ige ◽  
In Vitro System ◽  
Ige Antibodies ◽  
Total Ige ◽  
In Vitro Systems ◽  
Specific Ige Antibodies ◽  
A New Technique

Two in vitro systems of measuring total IgE and specific IgE antibodies were compared using serum of patients suspected of having allergic rhinitis due to inhalation of grass pollen. One in vitro system was the Phadebas PRIST and RAST. The accuracy of these tests has been well defined and they were used as the standard. The Acti*Tip Allerg*E and Allerg*ENS system is a new technique utilizing a similar solid-phase immunologic methodology with an enzyme-based detection system. It has some advantages primarily related to the mechanics of performing the test and marked reduction in time for obtaining results. The correlation between the Phadebas and Acti*Tip systems was highly significant for measuring total IgE and specific IgE antibodies for grasses in serum.

scholarly journals Flow cytometric analysis of in vitro activated basophils, specific IgE and skin tests in the diagnosis of pollen-associated food allergy

2005 ◽  
Vol 64B (1) ◽  
pp. 28-33 ◽  
Author(s):  
Didier G. Ebo ◽  
Margo M. Hagendorens ◽  
Chris H. Bridts ◽  
Annemie J. Schuerwegh ◽  
Luc S. De Clerck ◽  
...  
Keyword(s):  
Food Allergy ◽  
Flow Cytometric Analysis ◽  
Specific Ige ◽  
Skin Tests ◽  
Flow Cytometric
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