scholarly journals Microbiological safety and hygienic quality of camel meat at abattoir and retail houses in Jigjiga city, Ethiopia

2019 ◽  
Vol 13 (03) ◽  
pp. 188-194 ◽  
Author(s):  
Henok Ayalew Tegegne ◽  
Amare Berhanu ◽  
Yitbarek Getachew ◽  
Biresaw Serda ◽  
Dagmar Nölkes ◽  
...  
Keyword(s):  
Fecal Coliforms ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
E Coli ◽  
Microbiological Safety ◽  
Retail Meat ◽  
Camel Meat ◽  
Meat Samples ◽  
Campylobacter Spp

Introduction: Camel meat is a relatively new, emerging meat type that may serve as sources of foodborne pathogens to the consumer. Methodology: A cross-sectional study was conducted to determine the microbiological safety and quality of camel meat from an abattoir and retail houses in Jigjiga city, Ethiopia. A total of 140 camel carcass and retail meat samples (70 each) were examined for the presence and load of Staphylococcus aureus, Escherichia coli O157: H7, Listeria monocytogenes, Campylobacter spp., aerobic bacteria, fecal coliforms (FCs), and yeast and molds (Y&Ms). Presumptive isolates were confirmed using biochemical tests. Results: S. aureus and E. coli O157: H7 populations varied widely between carcasses at the abattoir and retail meat samples. S. aureus and E. coli O157:H7 were detected in 12.1 and 4.3% of the samples, respectively. E. coli O157:H7 counts were significantly higher in retail meat (4.21 ± 0.02) compared to the carcasses (3.99 ± 0.00) at the abattoir (P < 0.05). Out of 140 samples analyzed, 5% were positive for Campylobacter spp. The mean fecal coliforms, and yeast and molds counts were significantly higher in retail meat samples (6.17 ± 0.067 and 4.95 ± 0.067 log10 cfug-1, respectively). L. monocytogenes (11 cfug-1) were detected below the permissible limit (100 cfug-1). Conclusions: This study indicated that the further the process progress, the greater the risk of contamination to the product. Therefore, good hygienic practices at the abattoir and retail houses and strict slaughtering process should be prompted to enhance the overall safety and quality of camel meat.

Water Quality of Swimming Pools in Jerusalem

1993 ◽  
Vol 27 (7-8) ◽  
pp. 287-294 ◽  
Author(s):  
S. Lerman ◽  
O. Lev ◽  
A. Adin ◽  
E. Katzenelson
Keyword(s):  
Water Quality ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Swimming Pools ◽  
Indicator Organisms ◽  
Indicator Microorganisms ◽  
E Coli ◽  
Safe Water ◽  
Indirect Information

The Israel Ministry of Health is now revising its regulations for the assurance of safe water quality in public swimming pools. Since it is not possible to monitor each of the pathogenic microorganisms, it is often recommended to monitor indicator bacteria which provide indirect information on the water quality in the swimming pool. Three indicator microorganisms are often recommended: coliform counts (total coliforms, fecal coliforms or E. Coli), staphylococcus aureus and pseudomonas aeruginosa. A four year survey of the water quality of swimming pools in the Jerusalem District was conducted in order to determine whether the monitoring of all three indicators is necessary to assure safe water quality or is it sufficient to monitor only a single microorganism. A statistical analysis, conducted by using several different statistical techniques, reveals that the populations of the three indicator organisms are significantly interdependent but the correlations between each pair of these indicators are not sufficient to base a prediction of any of the organisms based on the measurements of the others. Therefore, it is concluded that monitoring of all three indicators should be recommended in order to provide an adequate picture of the water quality in swimming pools.

scholarly journals Quality of beef diaphragm meat in naturally occurring Sarcocystic infection in cattle

2018 ◽  
Vol 36 (No. 5) ◽  
pp. 378-385
Author(s):  
Vytautas Januskevicius ◽  
Grazina Januskeviciene ◽  
Gintare Zaborskiene
Keyword(s):  
Meat Quality ◽  
Harmful Effect ◽  
Coliform Bacteria ◽  
E Coli ◽  
Noticeable Increase ◽  
Physico Chemical ◽  
Infected Meat ◽  
Technological Quality ◽  
Meat Samples

The aim of this study was to investigate the possible harmful effect of Sarcocystis parasites on bovine diaphragm meat quality. Meat samples were collected from 120 bulls aged 20–24 months. Meat quality was investigated using microbiological and physico-chemical (RP-HPLC, GC) methods 48 hours after slaughter. Sarcocystis infection was associated with increased fat content, lightness L* and drip loss, and decreased ash and protein percentages. Infection also had a significant effect on the amount of amino acids (AAs), which slowly decreased as the number of sarcocysts increased. The total amount of AAs correlated with glutamic acid content (R = 0.966, P &lt; 0.05). Heavily infected samples contained significantly lower amounts of putrescine, histamine, spermine and spermidine (P &lt; 0.05) and<br /> a noticeable increase in the total count of aerobic microorganisms, but no change in the numbers of E. coli and coliform bacteria in comparison with no infected samples. Sarcocysts in beef diaphragms did not cause serious changes in the technological quality of the meat, but the biological quality of infected meat was reduced.  

scholarly journals Evaluation of a novel and rapid screening method for the detection of contaminated colistin-resistant bacteria in food

2020 ◽  
Author(s):  
Hanh Vu ◽  
Cornelia Appiah-Kwarteng ◽  
Kaori Tanaka ◽  
Ryuji Kawahara ◽  
Diep Thi Khong ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Method ◽  
High Speed ◽  
Screening Method ◽  
Rapid Screening ◽  
Resistant Bacteria ◽  
Practical Utility ◽  
E Coli ◽  
Retail Meat ◽  
Meat Samples

Abstract Background: The dissemination of colistin-resistant bacteria carrying the colistin-resistant mobile gene, mcr-1 threatens medical care worldwide. In particular, contamination of food with colistin-resistant bacteria accelerates the community dissemination of colistin-resistant bacteria. Therefore, monitoring of colistin-resistant bacteria in food is important for controlling resistant bacteria. Unfortunately, the conventional culture methods for detecting colistin-resistant bacteria are not practical for monitoring food saftey. Therefore, development of a simple and rapid method to detect food contamination with colistin-resistant bacteria is desirable as an effective means for preventing the dissemination of resistant bacteria, particularly colistin-resistant bacteria.Findings: We developed a simple and rapid method for detecting Escherichia coli harboring the mcr-1 colistin resistance gene using a high-speed real-time polymerase chain reaction (PCR). The entire procedure, from sample processing to finals results, was performed within one hour. The practical utility of this method was verified by analyzing 27 retail meat samples for the presence of colistin-resistant bacteria. The results of the developed method were in agreement with the results of culturing colistin-resistant E. coli from the meat samples, demonstrating its efficacy and usefulness.Conclusions: A simple and rapid real-time PCR-based screening method was developed for detecting E. coli harboring mcr-1 in food samples. The practical utility of the procedure was confirmed using retail meat samples, indicating its potential as a convenient and rapid method to detect bacterial contamination of food items, especially in developing communities.

scholarly journals THE DIFFERENCE OF E.COLI CONTENT IN THE CHICKEN MEAT IN THE SOUTH KEPUTRAN TRADITIONAL MARKET AND SUPERMARKET ' X ' OF SURABAYA CITY

2019 ◽  
Vol 14 (1) ◽  
pp. 24
Author(s):  
Athalla Permana ◽  
R. Bambang W
Keyword(s):  
Bacterial Contamination ◽  
Chicken Meat ◽  
Personal Hygiene ◽  
Food Handler ◽  
The South ◽  
Cross Sectional ◽  
E Coli ◽  
Traditional Market ◽  
The Difference ◽  
Meat Samples

Hygiene and sanitation practices in chicken influenced Eschericia coli bacterial contamination in food. A Study conducted by Sasmita and Juwita mentioned that there was positively E.coli content in chicken meat in supermarket. Thus, the purpose of this study was to assess the difference of E.Coli content in chicken meat and personal hygiene of food handler. It was an observational study with cross sectional approach. The population of this study included traditional market sellers and supermarkets. The sample of this study consisted of 14 samples of chicken meat in which 7 samples came from the South Keputran traditional market and other 7 samples came from Supermarket ‘X’. Moreover, 7 traditional market sellers and 2 supermarkets were involved to be examined. Variables of the study were E.coli content in chicken meat from Traditional Market of South Keputran and Supermarket ‘X’ and personal hygiene. Samples of chicken meat was done by accidental sampling. Data were collected through interview and observation, whereas the difference of E-Coli content was analyzed using statistical test. The results of this study indicated that one of chicken meat samples positively contained E.Coli bacteria, and no significant differences of the E.coli content were found on the chicken meat samples from both the Traditional Market of South Keputran and Supermarket ‘X’. The suggestion that can be given to Supermarket ‘X’ seller is to control and pay close attention to the sanitation process from suppliers to retails

scholarly journals Effect of Electron Beam Irradiation on Survival of Escherichia coli O157:H7 and Salmonella enterica serovar Thyphimurium in Minced Camel Meat during Refrigerated Storage

2019 ◽  
Author(s):  
A. Amiri ◽  
H. Zandi ◽  
H. Mozaffari Khosravi
Keyword(s):  
Escherichia Coli ◽  
Electron Beam ◽  
Electron Beam Irradiation ◽  
Escherichia Coli O157 ◽  
Refrigerated Storage ◽  
Beam Irradiation ◽  
E Coli ◽  
Serovar Typhimurium ◽  
Camel Meat ◽  
Meat Samples

Background: Electron beam irradiation is one of the effective ways to control foodborne pathogens. We evaluated the effect of electron beam irradiation on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Thyphimurium in minced camel meat during refrigerated storage. Methods: The meat samples were inoculated with E. coli O157:H7 and S. enterica serovar Thyphimurium and then irradiated with doses of 0, 1, 2, 3, and 5 kGy. The samples were stored at 4±1 °C and evaluated microbiologically up to 10 days. Data were analyzed using SPSS software version 18. Results: The microbial loads of minced camel meat samples were significantly reduced (p<0.0001) with increasing the dose of irradiation. The most effective dose was 5 kGy that highly reduced S. enterica serovar Typhimurium, and completely destroyed E. coli O157:H7. However, E. coli O157:H7 was more sensitive to electron beam irradiation than S. enterica serovar Typhimurium. Conclusion: Electron beam irradiation effectively reduced the population of both E. coli O157:H7 and S. enterica serovar Typhimurium in minced camel meat in a dose dependent manner.

Sleep, social behavior and food consumption of schoolchildren of a large Brazilian city

2020 ◽  
pp. 1-24
Author(s):  
Adriana Cândida da Silva ◽  
Érica Leandro Marciano Vieira ◽  
Luana Caroline dos Santos
Keyword(s):  
Food Consumption ◽  
Social Behaviour ◽  
Cross Sectional Study ◽  
Poor Sleep ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
Strengths And Difficulties ◽  
Before School ◽  
Sleep Talking

Abstract Objective: To characterize sleep and associated factors to their inadequacy, mainly social behaviour and food consumption of children and adolescents. Design: Cross-sectional study. Setting: Sleep information, social behaviour (Strengths and Difficulties Questionnaire), food consumption, demography, nutritional status, lifestyle and biochemical tests were investigated. Participants: Schoolchildren of the 4th grade of the municipal school system of a large Brazilian city. Results: A total of 797 schoolchildren, 50.9% was female, median of 9.7 (9.5-10.0) y old and energy consumption of 1819.7 (1429.9-2334.2) kcal. It was identified 31.6% of overweight and 76.8% reported insufficient weekly practice of physical activity. It was observed a median of 9.6 (8.9-10.5) h of sleep (lower values on weekdays: 9.3 vs 10.5h, P<0.001). In addition, 27% of individuals with inadequate sleep (<9h) enjoy longer screen time daily (≥2h/day) (P=0.05), inadequate bedtime (>22h) or adequate wake-up time (5-7h), study in the morning (P<0.001) and never take a shower before school (P<0.001). There was 9.9% of the sample with poor and very poor sleep quality and a greater probability of always sleep talking, have difficulty getting to sleep and inadequate social behaviour between these in relation to those with positive quality of sleep. There was no association of sleep with the other variables investigated. Conclusions: Sleep impairment contributed to changes in sleep and social behaviour in schoolchildren. The findings of this study may reinforce the importance of developing actions to promote adequate sleep and lifestyle at school age.

scholarly journals Isolation and Enumeration of Escherichia coli from Soil and Water

2020 ◽  
Vol 36 (2) ◽  
pp. 75-77
Author(s):  
Anindita Bhowmik ◽  
Sunjukta Ahsan
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Gas Production ◽  
Soil Samples ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Probable Number ◽  
E Coli ◽  
Dilution Series

Majority of the population of Bangladesh depend on tap or surface water as their source of water supply. This study was carried out to examine the microbial quality of both water and soil collected from different places using the multiple tube fermentation technique to determine coliform count by the most probable number (MPN) method in brilliant green lactose broth (BGLB) media.Inoculum from positive tubes of the presumptive test were further transferred on eosinemethylene blue (EMB) and MacConkey agar.The organisms isolated were further characterized using biochemical tests. Out of 93 water samples, 30 (32.26%) indicated the presence of lactose fermenter and gas producer in all 3 tubes of dilution series using inoculum quantities of 1.0, 0.1 and 0.01 ml, whereas out of 85 soil samples, 45 (52.94%) showed acid and gas production in all 3 tubes of dilution series.Among 85 soil samples, 40 samples that contained at least one positive in each dilution series and among 93 water samples, 31 samples that contained at least one positive in each dilution series were further re-identified with biochemical tests.This study showed 30.59% soil isolates and 26.88% water isolates were Escherichia coli which highlighted the fact that both water and soil act as a major reservoir of E.coli, which indicates possible fecal contamination as well as presence of potentially pathogenic E. coli. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 75-77

Minimally Processed Vegetable Salads: Microbial Quality Evaluation

2007 ◽  
Vol 70 (5) ◽  
pp. 1277-1280 ◽  
Author(s):  
HANS FRÖDER ◽  
CECÍLIA GERALDES MARTINS ◽  
KATIA LEANI OLIVEIRA de SOUZA ◽  
MARIZA LANDGRAF ◽  
BERNADETTE D. G. M. FRANCO ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Microbiological Quality ◽  
Positive Sample ◽  
Fecal Coliforms ◽  
Microbial Quality ◽  
Biochemical Tests ◽  
Minimally Processed ◽  
Increasing Demand ◽  
Minimally Processed Vegetables

The increasing demand for fresh fruits and vegetables and for convenience foods is causing an expansion of the market share for minimally processed vegetables. Among the more common pathogenic microorganisms that can be transmitted to humans by these products are Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella. The aim of this study was to evaluate the microbial quality of a selection of minimally processed vegetables. A total of 181 samples of minimally processed leafy salads were collected from retailers in the city of Sao Paulo, Brazil. Counts of total coliforms, fecal coliforms, Enterobacteriaceae, psychrotrophic microorganisms, and Salmonella were conducted for 133 samples. L. monocytogenes was assessed in 181 samples using the BAX System and by plating the enrichment broth onto Palcam and Oxford agars. Suspected Listeria colonies were submitted to classical biochemical tests. Populations of psychrotrophic microorganisms &gt;106 CFU/g were found in 51% of the 133 samples, and Enterobacteriaceae populations between 105 and 106 CFU/g were found in 42% of the samples. Fecal coliform concentrations higher than 102 CFU/g (Brazilian standard) were found in 97 (73%) of the samples, and Salmonella was detected in 4 (3%) of the samples. Two of the Salmonella-positive samples had &lt;102 CFU/g concentrations of fecal coliforms. L. monocytogenes was detected in only 1 (0.6%) of the 181 samples examined. This positive sample was simultaneously detected by both methods. The other Listeria species identified by plating were L. welshimeri (one sample of curly lettuce) and L. innocua (2 samples of watercress). The results indicate that minimally processed vegetables had poor microbiological quality, and these products could be a vehicle for pathogens such as Salmonella and L. monocytogenes.

scholarly journals Extraintestinal Escherichia coli Carrying Virulence Genes in Coastal Marine Sediments

2010 ◽  
Vol 76 (17) ◽  
pp. 5659-5668 ◽  
Author(s):  
G. M. Luna ◽  
C. Vignaroli ◽  
C. Rinaldi ◽  
A. Pusceddu ◽  
L. Nicoletti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Marine Sediments ◽  
Large Scale ◽  
Microbiological Quality ◽  
Genotypic Diversity ◽  
Coastal Sediments ◽  
Fecal Coliforms ◽  
Biochemical Tests ◽  
Term Survival ◽  
E Coli

ABSTRACT Despite the recognized potential of long-term survival or even growth of fecal indicators bacteria (FIB) in marine sediments, this compartment is largely ignored by health protection authorities. We conducted a large-scale study over approximately 50 km of the Marche coasts (Adriatic Sea) at depths ranging from 2 to 5 m. Total and fecal coliforms (FC) were counted by culture-based methods. Escherichia coli was also quantified using fluorescence in situ hybridization targeting specific 16S rRNA sequences, which yielded significantly higher abundances than culture-based methods, suggesting the potential importance of viable but nonculturable E. coli cells. Fecal coliforms displayed high abundances at most sites and showed a prevalence of E. coli. FC isolates (n = 113) were identified by API 20E, additional biochemical tests, and internal transcribed spacer-PCR. E. coli strains, representing 96% of isolates, were then characterized for genomic relatedness and phylogenetic group (A, B1, B2, and D) of origin by randomly amplified polymorphic DNA and multiplex-PCR. The results indicated that E. coli displayed a wide genotypic diversity, also among isolates from the same station, and that 44 of the 109 E. coli isolates belonged to groups B2 and D. Further characterization of B2 and D isolates for the presence of 11 virulence factor genes (pap, sfa/foc, afa, eaeA, ibeA, traT, hlyA, stx 1, stx 2, aer, and fyuA) showed that 90% of B2 and 65% of D isolates were positive for at least one of these. Most of the variance of both E. coli abundance and assemblage composition (>62%) was explained by a combination of physical-chemical and trophic variables. These findings indicate that coastal sediments could represent a potential reservoir for commensal and pathogenic E. coli and that E. coli distribution in marine coastal sediments largely depends upon the physical and trophic status of the sediment. We conclude that future sampling designs aimed at monitoring the microbiological quality of marine coastal areas should not further neglect the analysis of the sediment and that monitoring of these environments can be improved by including molecular methods as a complement of culture-based techniques.

Sand, soil, and pigeon droppings: sources of indicator bacteria in the waters of Hanauma Bay, Oahu, Hawaii

1995 ◽  
Vol 31 (5-6) ◽  
pp. 251-254 ◽  
Author(s):  
Robin Oshiro ◽  
Roger Fujioka
Keyword(s):  
Water Quality ◽  
Indicator Bacteria ◽  
Fecal Coliforms ◽  
Beach Sand ◽  
Sand Soil ◽  
E Coli ◽  
Pigeon Droppings ◽  
Sand Land ◽  
Land Runoff

To investigate the deteriorating bacteriological water quality of Hanauma Bay, Hawaii, samples of shoreline water, sand, land runoff, and mongoose and pigeon droppings were analysed for fecal coliforms, E. coli and enterococci. The results indicate that the major sources contributing to periodic high levels of bacteria in the waters of the Bay are contaminants of the beach sand, such as pigeon feces.

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