scholarly journals Rodents and other small mammal reservoirs in plague foci in northeastern Brazil

2017 ◽  
Vol 11 (05) ◽  
pp. 426-430 ◽  
Author(s):  
Erika de Cássia Vieira Da Costa ◽  
Marise Sobreira ◽  
Nilma Cintra Leal ◽  
Alzira Maria Paiva De Almeida

Introduction: Plague is an acute, infectious zoonotic disease, primarily of wild rodents and their fleas, that affects humans and other mammals. In Brazil, several plague foci are located in the northeast region. Plague surveillance based on monitoring of rodents was discontinued in 2007, and the current information on rodent populations is unsatisfactory. Our purpose was to update the information on rodents and other small mammals in plague foci in northeastern Brazil. Methodology: Nine surveys in the historically most important northeastern plague areas were conducted in 2013-2015. Results: In this study, 393 animals (13 rodent and four marsupial species) were entrapped. The plague bacterium Yersinia pestis was not detected in tissue sample cultures from the 225 animals that were analyzed. Eighty sera samples were analyzed for anti-F1 antibodies by hemagglutination (HA) and protein A ELISA tests, and all were negative, except for one marsupial, Monodelphis domestica, which was HA positive. Conclusions: Qualitative and quantitative differences in the animal populations were observed in the areas surveyed, and the antibody positive marsupial indicated that plague continues to circulate in the wild.

Author(s):  
Diego Leandro Reis da Silva Fernandes ◽  
Matheus Filgueira Bezerra ◽  
Bruna Mendes Duarte ◽  
Mayara Paes de França Silva ◽  
Hadassa de Almeida Souza ◽  
...  

The plague caused by the Yersinia pestis bacterium is primarily a flea-transmitted zoonosis of rodents that can also be conveyed to humans and other mammals. In this work, we analyzed the spatial and temporal distribution of rodents’ populations during active and quiescent periods of the plague in the municipality of Exu, northeastern Brazil. The geospatial analyses had shown that all rodent species occurred through the whole territory of the municipality with different hotspots for the risk of occurrence of the different species. Important fluctuation in the rodent populations was observed with a reduction in the wild rodent fauna following the end of a plague epidemic period, mostly represented by Necromys lasiurus and increase of the commensally species Rattus rattus. A higher abundance of rats might lead to an increased exposure of humans populations, favoring spillovers of plague and other rodent-borne diseases. Our analysis contributed to further highlight the role of the wild rodent species as the amplifier hosts and of the commensally rats (Rattus rattus) as the preserver hosts on the quiescent period on that transmission infection area.


2021 ◽  
Vol 6 (4) ◽  
pp. 195
Author(s):  
Diego Leandro Reis da Silva Fernandes ◽  
Matheus Filgueira Bezerra ◽  
Bruna Mendes Duarte ◽  
Mayara Paes de França Silva ◽  
Hadassa de Almeida Souza ◽  
...  

The plague caused by the Yersinia pestis bacterium is primarily a flea-transmitted zoonosis of rodents that can also be conveyed to humans and other mammals. In this work, we analyzed the spatial and temporal distribution of rodent populations during epizootic and enzootic periods of the plague in the municipality of Exu, northeastern Brazil. The geospatial analyses showed that all the rodent species appeared through the whole territory of the municipality, with different occurrence hotspots for the different species. Important fluctuations in the rodent populations were observed, with a reduction in the wild rodent fauna following the end of a plague epizootic period, mostly represented by Necromys lasiurus and an increase in the commensal species Rattus rattus. A higher abundance of rats might lead to an increased exposure of human populations, favoring spillovers of plague and other rodent-borne diseases. Our analysis highlights the role of wild rodent species as amplifier hosts and of commensal rats (R. rattus) as preserver hosts in the enzootic period of a specific transmission infection area.


2019 ◽  
Vol 100 (4) ◽  
pp. 1364-1373 ◽  
Author(s):  
Gabby Neves Guilhon ◽  
Caryne Braga ◽  
João Alves De Oliveira

Abstract Marsupials have short gestation periods, with altricial neonates completing their development attached to a teat, a condition that induces remarkable structural changes in the inguinal region of reproductive females. In this study, we analyzed the morphological variation in the inguinal region of female museum specimens of Monodelphis domestica in search for external traits reminiscent of their reproductive condition when collected. We examined 427 taxidermied skins of wild females from northeastern Brazil and classified their inguinal region by color, density of fur, shape, and the presence of spots and teats. We determined relative age based on tooth eruption and wear, to infer the chronological order of inguinal changes. These conditions were computed for each age class and for each month over 4 years for samples from two mesoregions in northeastern Brazil, identified from climatic characteristics. Four distinct morphological patterns recognized in the inguinal region of females were associated with the following reproductive conditions: 1) non-lactating: general shape of the pelvic region indistinct from the rest of body (straight), inguinal region lacking teats, same fur density and color (grayish) as the rest of the body; 2) pre-lactating: pelvic and inguinal regions similar to non-lactating, but yellowish fur and presence of small teats; 3) early-lactating: inguinal region with well-developed teats, fur yellowish (rarely whitish) and less dense, and form of the pelvic region rounded; and 4) late or post-lactating: inguinal region with well-developed teats and dark orange and less dense fur, pelvic region rounded. Lactating females were only recorded from age class 4 on. We also found indications that pelage changes in the inguinal region do not revert after reproduction in the wild. Monthly frequencies of the different conditions recorded for lactating females corroborate previous studies that suggested that M. domestica breeds all year long in northeastern Brazil. Marsupiais caracterizam-se por apresentar um curto período de gestação, com neonatos altriciais completando seu desenvolvimento ligados à teta, uma condição que induz mudanças estruturais marcantes na região inguinal de fêmeas reprodutivas. No presente estudo analisamos a variação morfológica da região inguinal de fêmeas taxidermizadas de Monodelphis domestica, buscando traços reminiscentes de suas condições reprodutivas quando coletadas. Examinamos 427 peles taxidermizadas de fêmeas silvestres obtidas no Nordeste do Brasil, e classificamos a região inguinal pela cor, densidade de pelos, presença de manchas e tetas, e forma da região pélvica. Determinamos a idade relativa com base na erupção e no desgaste dentário, para inferir a ordem cronológica das mudanças inguinais. Essas condições foram registradas para cada idade e por cada mês ao longo de 4 anos para amostras de duas mesorregiões no Nordeste do Brasil, identificadas com base em características climáticas. Quatro padrões morfológicos distintos reconhecidos na região inguinal das fêmeas foram associadas às seguintes condições reprodutivas: 1) não-lactante: forma geral da região pélvica indistinta do resto do corpo (retas), região inguinal sem tetas e mesma cor e densidade da pelagem (cinza) que o resto do corpo; 2) pré-lactante: regiões inguinal e pélvica similares às do padrão não-lactante, mas pelagem amarelada e presença de tetas pequenas; 3) lactante inicial: região inguinal com tetas bem desenvolvidas, pelagem inguinal amarelada (raramente branca) e menos densa, forma da região pélvica arredondada; 4) lactante tardia ou pós-lactante: região inguinal com tetas bem desenvolvidas, pelagem laranja escura e menos densa, região pélvica arredondada. Fêmeas lactantes foram observadas apenas da classe de idade 4 em diante. Encontramos evidências de que a mudança da pelagem na região inguinal não é revertida depois da reprodução na natureza. Frequências mensais das diferentes condições reprodutivas das fêmeas observadas corroboram estudos prévios que sugerem que M. domestica pode se reproduzir ao longo de todo o ano no Nordeste do Brasil.


Author(s):  
Mohamed El-Diasty ◽  
◽  
Rana El-Said ◽  
Adel Abdelkhalek ◽  
◽  
...  

Brucellosis is an endemic disease among livestock and humans in Egypt. Sheep are the most common type of livestock ruminant in Egypt and considered the fundamental etiology for spreading and maintaining B. melitensis either in human being or animal populations. In the current study, we investigated the seroprevalence of brucellosis in sheep herds reared in Bilqase, one of the biggest cities at Dakahlia governorate in Egypt's Delta region. In total, 610 sheep from seven herds were investigated. Anti-Brucella antibodies were detected in 48 (7.8%) samples tested by Buffered Acidified Plate Antigen Test (BAPAT), in 44 (7.2%) samples tested by Rose Bengal Plate Test (RBPT) and in 41 (6.7%) samples tested by Milk Ring Test (MRT). The isolation rate was 29.6% (16 out of 54 examined samples). Brucella organism was isolated from three aborted fetuses, one tissue sample of slaughtered serologically positive ewe and 12 milk samples. The Abortus Melitensis Ovis Suis-PCR (AMOS-PCR) confirmed all Brucella strains as B. melitensis. More than three successive negative serological tests are required to declare that the infected herd is free from brucellosis. In conclusion, no single serological test could conclusively diagnose brucellosis in endemic areas. Confirmation of results with molecular diagnosis or culture is indispensable in diagnosis. B. melitensis was the prevalent serotype among sheep in Dakahlia governorate


2013 ◽  
Vol 22 (4) ◽  
pp. 519-524 ◽  
Author(s):  
Sócrates Fraga da Costa Neto ◽  
Vinicius Menezes Tunholi Alves ◽  
Victor Menezes Tunholi Alves ◽  
Juberlan Silva Garcia ◽  
Marcos Antônio José dos Santos ◽  
...  

The South American water rat Nectomys squamipes is a wild mammal reservoir of Schistosoma mansoni in Brazil. In the present study, wild rodents were collected in the field and categorized into two groups: infected and uninfected by S. mansoni. Blood was collected to analyze changes in the serum glucose level (mg/dL) and liver fragments were used to determine the hepatic glycogen content (mg of glucose/g tissue). The histological examination showed inflammatory granulomatous lesions in different phases of development in the liver of rodents naturally infected with S. mansoni, in some cases with total or partial occlusion of the vascular lumen. Early lesions were characterized by the presence of inflammatory infiltrate around morphologically intact recently deposited eggs. Despite the significance of these histological lesions, the biochemical changes differed in extent. N. squamipes naturally infected byS. mansoni showed no variation in hepatic glycogen reserves. These findings were accompanied by a significant increase in plasma glucose contents, probably as a consequence of amino acids deamination, which are degraded, resulting in the formation of intermediates used as precursors for the glucose formation, without compromising the reserves of liver glycogen. In the wild, naturally infected N. squamipes can maintainS. mansoni infections without undergoing alterations in its carbohydrate metabolism, which minimizes the deleterious effects of S. mansoni.


World Science ◽  
2020 ◽  
Vol 1 (3(55)) ◽  
pp. 17-25
Author(s):  
Нода О. М. ◽  
Закора О. В. ◽  
Рязанова О. Ю.

Modern consumers attach increasing importance to tactile sensations and the general perception of the surface of fabrics, therefore, the study of the structure and properties of woven textures is an urgent task of textile production. In textile technologies, organoleptic research methods are used mainly, which give a subjective visual and tactile assessment of the surface of the tissues. Therefore, there was a need to develop an instrumental method for determining the texture of fabrics, which was implemented on the basis of the existing method for studying the texture of hard surfaces. It is based on the idea of creating a constant mode of illumination of the object of observation, which is a tissue sample. The proposed method is based on the use of a special optical device for determining the qualitative and quantitative characteristics of textures and is a viewing chamber containing a mechanism for changing the lighting mode and a mechanism for capturing tissue images. For an objective assessment of the state of the surface of the tissues, the invoice indicator is calculated − the invoice coefficient. The implementation of the developed method allows to increase the efficiency of the processes of design-design of fabrics, to predict the appearance of fabrics and to ensure compliance with the texture of the fabric of the model of the garment.


2017 ◽  
Vol 117 (2) ◽  
pp. 566-581 ◽  
Author(s):  
James C. Dooley ◽  
Michaela S. Donaldson ◽  
Leah A. Krubitzer

The functional organization of the primary visual area (V1) and the importance of sensory experience in its normal development have been well documented in eutherian mammals. However, very few studies have investigated the response properties of V1 neurons in another large class of mammals, or whether sensory experience plays a role in shaping their response properties. Thus we reared opossums ( Monodelphis domestica) in normal and vertically striped cages until they reached adulthood. They were then anesthetized using urethane, and electrophysiological techniques were used to examine neuronal responses to different orientations, spatial and temporal frequencies, and contrast levels. For normal opossums, we observed responses to the temporal and spatial characteristics of the stimulus to be similar to those described in small, nocturnal, eutherian mammals such as rats and mice; neurons in V1 responded maximally to stimuli at 0.09 cycles per degree and 2.12 cycles per second. Unlike other eutherians, but similar to other marsupials investigated, only 40% of the neurons were orientation selective. In stripe-reared animals, neurons were significantly more likely to respond to vertical stimuli at a wider range of spatial frequencies, and were more sensitive to gratings at lower contrast values compared with normal animals. These results are the first to demonstrate experience-dependent plasticity in the visual system of a marsupial species. Thus the ability of cortical neurons to alter their properties based on the dynamics of the visual environment predates the emergence of eutherian mammals and was likely present in our earliest mammalian ancestors.NEW & NOTEWORTHY These results are the first description of visual response properties of the most commonly studied marsupial model organism, the short-tailed opossum ( Monodelphis domestica). Further, these results are the first to demonstrate experience-dependent plasticity in the visual system of a marsupial species. Thus the ability of cortical neurons to alter their properties based on the dynamics of the visual environment predates the emergence of eutherian mammals and was likely present in our earliest mammalian ancestors.


Development ◽  
1990 ◽  
Vol 109 (3) ◽  
pp. 699-703
Author(s):  
P.J. Baker ◽  
H.D. Moore ◽  
L.M. Penfold ◽  
A.M. Burgess ◽  
U. Mittwoch

A quantitative and histological study of the gonads of newborn grey short-tailed opossums, Monodelphis domestica, is described. The pups were karyotyped, and comparisons were made within litters segregating for XX and XY sex chromosomes. A total of four litters including 25 pups were available. On the day of birth, developing testes were significantly larger than the ovaries of litter mates, and testes could be histologically distinguished by the formation of sex cords and a tunica albuginea. The data suggest that in this marsupial species gonadal differentiation may be initiated in utero.


1990 ◽  
Vol 259 (3) ◽  
pp. E384
Author(s):  
S A Rivkees ◽  
S M Reppert

In a marsupial species, the gray short-tailed opossum (Monodelphis domestica), the suprachiasmatic nuclei (SCN), the site of a circadian clock, are formed postnatally and begin oscillating as a circadian clock on day 20. In this study, we examined how the timing (phase) of the SCN clock in the developing opossum is coordinated to the environmental light-dark cycle. When pups were reared from birth in darkness by intact dams, the circadian phases in SCN metabolic activity (monitored by 2-deoxy-D-[14C]glucose autoradiography) in 27-day-old pups were desynchronized. When pups were reared in a light-dark cycle that was 12 h out of phase with the circadian time of blinded dams, the pattern of SCN metabolic activity on day 20 was rhythmic and in phase with the light-dark cycle but out of phase with the circadian time of the dam. On day 20, retina-mediated light activation of SCN metabolic activity was also demonstrated, and anterograde tract-tracing studies revealed the presence of the retinohypothalamic tract within the SCN. These results show there is no influence of the opossum dam on the timing of the pup's biological clock. Instead, from the inception of the daily rhythm in SCN metabolic activity, its timing is regulated by retina-mediated light-dark entrainment.


2014 ◽  
Vol 20 (1) ◽  
pp. 108
Author(s):  
A C Gsell ◽  
M N H Seabrook-Davison ◽  
D H Brunton

invasions to New Zealand’s unique biodiversity, ‘pest-free’ offshore and mainland island refuges have been created. The success of this approach depends on the long term maintenance of the pest-free status of these refuges. Because the occurrence of rodent incursions is an on-going risk, early detection and elimination of invading animals is crucial. We conducted field-based experiments to determine if lures of live female and male laboratory Norway rats (Rattus norvegicus) held in cages in the wild, and lures consisting of urine-soaked rodent bedding could be used to detect the presence of wild rodents. We found that the use of live rodents and bedding significantly increased the probability of detecting wild rodents, although we were not able to determine the sex, age or number of wild rodents attracted to each live rodent station. We also found that wild rats were equally attracted to the scent of male and female rats i.e., lures and bedding. Our approach provides potential as a tool for early detection of rodents in vulnerable refuges and we suggest that further research is needed to investigate the feasibility of developing rat scented monitoring stations.


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