scholarly journals Investigation of clonal relationships of K. pneumoniae isolates from neonatal intensive care units by PFGE and rep-PCR

2015 ◽  
Vol 9 (08) ◽  
pp. 829-836 ◽  
Author(s):  
Mehmet Koroglu ◽  
Ahmet Ozbek ◽  
Tayfur Demiray ◽  
Taner Hafizoglu ◽  
Ertugrul Guclu ◽  
...  
Keyword(s):  
Intensive Care ◽  
Neonatal Intensive Care ◽  
Molecular Techniques ◽  
Discriminatory Power ◽  
Bacterial Strains ◽  
Rdna Sequencing ◽  
Epidemic Period ◽  
Unrelated Strain ◽  
One Year ◽  
Relationship Of

Introduction: Clonal relationships of Klebsiella pneumoniae strains obtained during an epidemic and after a one-year post-epidemic (non-epidemic) period in the same neonatal intensive care unit (NICU) using pulsed-field gel electrophoresis (PFGE) and repetitive polymerase chain reaction (rep-PCR) by the DiversiLab (DL) system were investigated, and the results of both molecular techniques were evaluated. Methodology: Fifteen K. pneumoniae strains were included in this study. All identified bacterial strains were confirmed by 16S rDNA sequencing and analyzed by PFGE and the DL system. Results: According to the PFGE results, 15 isolates showed 10 different band profiles. Nine of these 15 isolates were included in one of the formed clusters, and the remaining six isolates were not included in any of them. According to the DL system results, 15 isolates showed two different clusters, with three strains in one cluster and four strains in the other. The remaining strains could not be placed any one of the clusters. PFGE was used as the gold standard based on its strong genetic discriminatory power. The DL system results showed that  PFGE missed the relationship of the two epidemic-related strains and demonstrated one epidemic-unrelated strain to be epidemic related. Conclusions: Both systems may easily be used for clonal relationships of K. pneumoniae strains. The DL system was clearly more rapid and convenient than PFGE, but its discriminatory power seemed to be inferior to that of PFGE based on 15 K. pneumoniae strains.

scholarly journals Chromosomal Microarray Testing in NEC: A Case Report

2016 ◽  
Vol 5 (3) ◽  
pp. 34 ◽  
Author(s):  
Sathyaprasad C Burjonrappa ◽  
David Schwartzberg
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care ◽  
Chromosomal Microarray ◽  
Chromosomal Microarray Analysis ◽  
Gut Microflora ◽  
Cytokine Release ◽  
The Common ◽  
One Year ◽  
Emergent Surgery

Necrotizing enterocolitis (NEC) remains the most common reason for emergent surgery in the neonatal intensive care unit. The common pathophysiology in all NEC involves alteration in gut microflora, abnormal blood supply to the intestine, and uncontrolled cytokine release. We report a full-term neonate who developed NEC. The neonate had surgical resection of approximately 120cms of bowel. After an initial proximal jejunostomy she underwent a successful jejuno-ileal anastomosis with preservation of her ileocolic valve at 6 weeks of age. A little more than one year of age, she is being weaned off her parenteral nutrition (PN) as her bowel adaptation continues. A chromosomal microarray analysis (CMA) resulted in the identification of a 15q13.3 microdeletion.

scholarly journals Respiratory compliance of newborns after birth and their short-term outcomes

2017 ◽  
Vol 4 (2) ◽  
pp. 620
Author(s):  
Pramila Verma ◽  
Ashish Kalraiya
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
Respiratory Support ◽  
Short Term ◽  
Term Outcome ◽  
Short Term Outcome ◽  
High Flow Oxygen ◽  
One Year

Background: Worldwide approximately one million babies die per year due to asphyxia out of which about one third (approximately 3 lakhs) is contributed by our country. The objective of this study was to determine the requirement of respiratory support in newborn babies and their short-term outcomes.Methods: This retrospective observational study was conducted over one year at PCMS and RC Bhopal. Babies who were delivered in PCMS and required respiratory support at birth were enrolled in the study. The medical records of all these babies for resuscitation measures, requirement of respiratory support after hospitalization to the neonatal intensive care unit and their short-term outcome were recorded on a pre-designed study proforma.Results: During post-resuscitation care in neonatal intensive care unit, 55.5% (30/54) babies required respiratory support. Among them 7.4% (4/54) were supplemented with oxygen for a few hrs, 22.2% (12/54) required positive end expiratory pressure and put on high flow oxygen and air mixture for 24-48 hours. While 25.9% (14/54) newborns required mechanical ventilation for 3-7 days and then switched over to CPAP for the next 24-48 hrs. Out of 54 babies eight babies expired while ten babies went against medical advice. Remaining 36 babies were discharged, of which five babies developed hypoxic ishchaemic encephalopathy.Conclusions: It is evident from the present study that half of the resuscitated babies further required respiratory support in the NICU. We also concluded that three fourth of the newborns were discharged and had normal short term outcome.

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