scholarly journals Phytochemical-induced reduction of pulmonary inflammation during Klebsiella pneumoniae lung infection in mice

2014 ◽  
Vol 8 (07) ◽  
pp. 838-844 ◽  
Author(s):  
Shruti Bansal ◽  
Sanjay Chhibber
Keyword(s):  
Lung Tissue ◽  
Histopathological Examination ◽  
Curcuma Longa ◽  
Pulmonary Inflammation ◽  
Bacterial Load ◽  
Neutrophil Infiltration ◽  
Lung Infection ◽  
Control Group ◽  
Inflammatory Parameters ◽  
Anti Inflammatory

Introduction: Curcumin, a polyphenol derived from the herb Curcuma longa, has number of antioxidant, anti-inflammatory, antimicrobial, and anti-carcinogenic activities. Its anti-inflammatory property was here studied alone and in combination with clarithromycin in a mouse model of acute inflammation. Methodology: A total of 80 mice divided into four groups were used. Mice receiving curcumin and/or clarithromycin were fed orally with curcumin (150 mg/kg/day) for 15 days prior to infection, whereas clarithromycin was administered orally (30 mg/kg/day) 12 hours post infection. Simultaneously, the control group receiving only infection but no treatment was also set up. Bacterial load estimation, histopathological examination and analysis of inflammatory parameters was performed on various days for all groups. Results: Intranasal inoculation of bacteria resulted in significant increase in neutrophil infiltration along with increased production of various inflammatory mediators (malondialdehyde, myeloperoxidase, nitric oxide, TNFα) in lung tissue. Clarithromycin treatment significantly decreased the bacterial load and other inflammatory components in infected mice, but animals receiving curcumin alone or in combination with clarithromycin showed a much more significant (p < 0.05) reduction in neutrophil influx along with reduced levels of various inflammatory parameters. Though treatment with curcumin did not reduce the bacterial load, in combination with clarithromycin, both bacterial proliferation and lung tissue damage were checked. Conclusions: Though clarithromycin, because of its associated side effects, may not be the preferred treatment, it can be used in conjunction with curcumin. The latter as an adjunct therapy will help to down regulate the exaggerated state of immune response during acute lung infection.

scholarly journals Curcumin alone and in combination with augmentin protects against pulmonaryinflammation and acute lung injury generated during Klebsiella pneumoniae B5055-induced lung infection in BALB/c mice

2010 ◽  
Vol 59 (4) ◽  
pp. 429-437 ◽  
Author(s):  
Shruti Bansal ◽  
Sanjay Chhibber
Keyword(s):  
Klebsiella Pneumoniae ◽  
Mouse Model ◽  
Lung Tissue ◽  
Inflammatory Mediators ◽  
Bacterial Load ◽  
Neutrophil Infiltration ◽  
Lung Infection ◽  
Control Group ◽  
Intranasal Route ◽  
Anti Inflammatory

Acute lung injuries due to acute lung infections remain a major cause ofmortality. Thus a combination of an antibiotic and a compound with immunomodulatoryand anti-inflammatory activities can help to overcome acute lung infection-inducedinjuries. Curcumin derived from the rhizome of turmeric has been used fordecades and it exhibits anti-inflammatory, anti-carcinogenic, immunomodulatoryproperties by downregulation of various inflammatory mediators. Keeping theseproperties in mind, we investigated the anti-inflammatory properties of curcuminin a mouse model of acute inflammation by introducing Klebsiella pneumoniae B5055 into BALB/c mice via the intranasal route. Intranasal instillationof bacteria in this mouse model of acute pneumonia-induced inflammation resultedin a significant increase in neutrophil infiltration in the lungs along withincreased production of various inflammatory mediators [i.e. malondialdehyde (MDA),myeloperoxidase (MPO), nitric oxide (NO), tumour necrosisfactor (TNF)-α] in the lung tissue. The animalsthat received curcumin alone orally or in combination with augmentin, 15 daysprior to bacterial instillation into the lungs via the intranasal route, showeda significant (P <0.05) decrease in neutrophil influxinto the lungs and a significant (P <0.05) decreasein the production of MDA, NO, MPO activity and TNF-α levels.Augmentin treatment alone did not decrease the MDA, MPO, NO and TNF-α levels significantly (P >0.05) as compared tothe control group. We therefore conclude that curcumin ameliorates lung inflammationinduced by K. pneumoniae B5055 without significantly (P <0.05) decreasing the bacterial load in the lung tissue whereasaugmentin takes care of bacterial proliferation. Hence, curcumin can be usedas an adjunct therapy along with antibiotics as an anti-inflammatory or animmunomodulatory agent in the case of acute lung infection.

scholarly journals Pulmonary prophylactic impact of melatonin and/or quercetin: A novel therapy for inflammatory hypoxic stress in rats

2017 ◽  
Vol 67 (1) ◽  
pp. 125-135 ◽  
Author(s):  
Nouf M. Al-Rasheed ◽  
Laila Fadda ◽  
Hala A. Attia ◽  
Iman A. Sharaf ◽  
Azza M. Mohamed ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Lung Tissue ◽  
Inflammatory Mediators ◽  
Histopathological Examination ◽  
Serum Levels ◽  
Control Group ◽  
Novel Therapy ◽  
Protein Expressions ◽  
Pre Treatment

AbstractThe study aims to compare, through histological and biochemical studies, the effects of quercetin, melatonin and their combination in regulation of immuno-inflammatory mediators and heat shock protein expressions in sodium nitrite induced hypoxia in rat lungs. The results revealed that NaNO2injection caused a significant decrease in Hb in rats, while serum levels of TNF-α, IL-6 and CRP, VEGF and HSP70 were elevated compared to the control group. Administration of melatonin, quercetin or their combination before NaNO2injection markedly reduced these parameters. Histopathological examination of the lung tissue supported these biochemical findings. The study suggests that melatonin and/or quercetin are responsible for lung tissue protection in hypoxia by downregulation of immuno-inflammatory mediators and heat shock protein expressions. Pre-treatment of hypoxic animals with a combination of melatonin and quercetin was effective in modulating most of the studied parameters to near-normal levels.

Antinociceptive and anti-inflammatory effects of electroacupuncture on experimental arthritis of the rat temporomandibular joint

2012 ◽  
Vol 90 (4) ◽  
pp. 395-405 ◽  
Author(s):  
Delane Viana Gondim ◽  
Jéssyca Lima Costa ◽  
Suellen Sousa Rocha ◽  
Gerly Anne de Castro Brito ◽  
Ronaldo de Albuquerque Ribeiro ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
Temporomandibular Joint ◽  
Sham Group ◽  
Neutrophil Migration ◽  
Low Frequency ◽  
Control Group ◽  
Histopathological Analysis ◽  
Myeloperoxidase Activity ◽  
Inflammatory Parameters ◽  
Anti Inflammatory

This study investigated the antinociceptive and anti-inflammatory effects of electroacupuncture (EA) on zymosan-induced acute arthritis of the rat temporomandibular joint (TMJ). Male Wistar rats were injected with saline or zymosan (control group; 2 mg) into the left TMJ. Low frequency EA (10 Hz, 30 min) was performed at acupoints (LI4, LI11, ST36, ST44) or sham points 2 h after or 1 h before zymosan administration. Mechanical hypernociception was accessed by the electronic Von Frey method after zymosan administration. Rats were sacrificed 6 h after zymosan administration and the joint was removed for histopathological analysis, myeloperoxidase activity assessment, vascular permeability observations, and immunohistochemical verification of inflammatory mediators. The results showed that EA inhibited zymosan-induced hypernociception, compared with the control group and with the sham group (p < 0.05). The results showed that EA inhibited inflammatory parameters such as neutrophil migration, vascular permeability, and tumour necrosis factor α (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) expression in the TMJ compared with the sham group (p < 0.05). Histopathological analysis showed that EA significantly inhibited edema and periarticular infiltration (p < 0.05) compared with the control and sham groups. EA at acupoints produced antinociceptive and anti-inflammatory effects on zymosan-induced arthritis in the rat TMJ.

scholarly journals Adipose-Derived Mesenchymal Stem Cells Ameliorating Pseudomonas aeruginosa–induced Acute Lung Infection via Inhibition of NLRC4 Inflammasome

2021 ◽  
Vol 10 ◽  
Author(s):  
Lu-lu Li ◽  
Ying-gang Zhu ◽  
Xin-ming Jia ◽  
Dong Liu ◽  
Jie-ming Qu
Keyword(s):  
Stem Cells ◽  
Pseudomonas Aeruginosa ◽  
Mesenchymal Stem Cells ◽  
Lung Tissue ◽  
Pulmonary Infection ◽  
Bacterial Load ◽  
Lung Infection ◽  
Inflammasome Activation ◽  
Macrophage Phagocytosis

BackgroundPseudomonas aeruginosa (PA) is one of the most common Gram-negative bacteria causing hospital-acquired pulmonary infection, with high drug resistance and mortality. Therefore, it is urgent to introduce new non-antibiotic treatment strategies. Mesenchymal stem cells (MSCs), as important members of the stem cell family, were demonstrated to alleviate pathological damage in acute lung injury. However, the potential mechanism how MSC alleviate acute lung infection caused by PA remains unclear.ObjectiveThe purpose of this study was to investigate the effects of Adipose-derived mesenchymal stem cells (ASCs) on acute pulmonary infections and the possible mechanisms how ASCs reduce pulmonary inflammation induced by PA.MethodsThe therapeutic and mechanistic effects of ASCs on PA pulmonary infection were evaluated respectively in a murine model as well as in an in vitro model stimulated by PA and co-cultured with ASCs.Results1. ASCs treatment significantly reduced the bacterial load, inflammation of lung tissue and histopathological damage by PA. 2. PA infection mainly activated Nod-like receptor containing a caspase activating and recruitment domain 4 (NLRC4) inflammasome in the lung of mice. ASCs attenuated acute lung infection in mice by inhibiting NLRC4 inflammasome activation. 3. NLRC4−/− mice showed a significant improvement in survival rate and lung bacterial load after PA infection. 4. ASCs mainly increased expression and secretion of STC‐1 in response to PA‐stimulated NLRC4 inflammasome activation.ConclusionsPA infection attenuated macrophage phagocytosis through activation of NLRC4 inflammasome in macrophages, which eventually led to pulmonary inflammatory damage in mouse; ASCs reduced the activation of NLRC4 inflammasome in macrophages induced by PA infection, thereby increasing the phagocytic ability of macrophages, and ultimately improving lung tissue damage in mouse; ASCs may inhibit NLRC4 inflammasome through the secretion of STC-1.

scholarly journals Anti-Inflammatory, Antioxidant, and Antifibrotic Effects of Gingival-Derived MSCs on Bleomycin-Induced Pulmonary Fibrosis in Mice

2021 ◽  
Vol 23 (1) ◽  
pp. 99
Author(s):  
Xishuai Wang ◽  
Shiyu Zhao ◽  
Junhui Lai ◽  
Weijun Guan ◽  
Yang Gao
Keyword(s):  
Pulmonary Fibrosis ◽  
Pulmonary Oedema ◽  
Lung Tissue ◽  
Lysophosphatidic Acid ◽  
Pulmonary Inflammation ◽  
Apoptotic Index ◽  
Expression Levels ◽  
Tnf Α ◽  
Severity Of Injury ◽  
Anti Inflammatory

Background: Mesenchymal stem cell (MSC) intervention has been associated with lung protection. We attempted to determine whether mouse gingival-derived mesenchymal stem cells (GMSCs) could protect against bleomycin-induced pulmonary fibrosis. Methods: Mice were divided into three groups: control (Con), bleomycin (Bl), and bleomycin + MSCs (Bl + MSCs). Mice were treated with 5 mg/kg bleomycin via transtracheal instillation to induce pulmonary fibrosis. We assessed the following parameters: histopathological severity of injury in the lung, liver, kidney, and aortic tissues; the degree of pulmonary fibrosis; pulmonary inflammation; pulmonary oedema; profibrotic factor levels in bronchoalveolar lavage fluid (BALF) and lung tissue; oxidative stress-related indicators and apoptotic index in lung tissue; and gene expression levels of IL-1β, IL-8, TNF-α, lysophosphatidic acid (LPA), lysophosphatidic acid receptor 1 (LPA1), TGF-β, matrix metalloproteinase 9 (MMP-9), neutrophil elastase (NE), MPO, and IL-10 in lung tissue. Results: GMSC intervention attenuated bleomycin-induced pulmonary fibrosis, pulmonary inflammation, pulmonary oedema, and apoptosis. Bleomycin instillation notably increased expression levels of the IL-1β, IL-8, TNF-α, LPA, LPA1, TGF-β, MMP-9, NE, and MPO genes and attenuated expression levels of the IL-10 gene in lung tissue, and these effects were reversed by GMSC intervention. Bleomycin instillation notably upregulated MDA and MPO levels and downregulated GSH and SOD levels in lung tissue, and these effects were reversed by GMSC intervention. GMSC intervention prevented upregulation of neutrophil content in the lung, liver, and kidney tissues and the apoptotic index in lung tissue. Conclusions: GMSC intervention exhibits anti-inflammatory and antioxidant capacities. Deleterious accumulation of neutrophils, which is reduced by GMSC intervention, is a key component of bleomycin-induced pulmonary fibrosis. GMSC intervention impairs bleomycin-induced NE, MMP-9, LPA, APL1, and TGF-β release.

scholarly journals Brazilian Green Propolis: Anti-Inflammatory Property by an Immunomodulatory Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Joleen Lopes Machado ◽  
Anne Karine Martins Assunção ◽  
Mayara Cristina Pinto da Silva ◽  
Aramys Silva dos Reis ◽  
Graciomar Conceição Costa ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Pulmonary Inflammation ◽  
Oral Route ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Water Control ◽  
Cotton Pellet Granuloma ◽  
Anti Inflammatory ◽  
Acute And Chronic Inflammation ◽  
Cytokine Quantification

The immunomodulatory and anti-inflammatory activities of green propolis extracts fromApis melliferawere investigated using acute and chronic inflammation models. Swiss mice were anesthetized and a cotton pellet granuloma was implanted in subcutaneous tissue. Then the mice were divided into six groups and received apyrogenic water or different propolis extracts by oral route (5 mg/kg). According to the treatment the groups were designated as E1A, E1B, E10, E11, and E12. The control group received apyrogenic water. The treatment was performed by six days when the mice were killed. The blood and the bronchoalveolar lavage (BAL) were collected to measure the leukocyte recruitment. In acute pulmonary inflammation, Balb/c mice received lipopolysaccharide (LPS) ofEscherichia coliby intranasal route for three days. Concomitantly the mice received by oral route apyrogenic water (control) or E10 and E11 propolis extracts. BAL was performed to assess the inflammatory infiltrate and cytokine quantification. The results showed that the E11 extract has anti-inflammatory property in both models by the inhibition of proinflammatory cytokines and increase of anti-inflammatory cytokines suggesting an immunomodulatory activity.

scholarly journals Bees’ Honey Attenuation of Metanil-Yellow-Induced Hepatotoxicity in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Abdulrahman L. Al-Malki ◽  
Ahmed Amir Radwan Sayed
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Markers ◽  
Histopathological Examination ◽  
Fatty Degeneration ◽  
Control Group ◽  
Cytoplasmic Vacuolization ◽  
Liver Injuries ◽  
Metanil Yellow ◽  
Anti Inflammatory

The present study aims to investigate the protective effect of bees’ honey against metanil-yellow-induced hepatotoxicity in rats. Rats were divided into 7 groups: control group; three groups treated with 50, 100, and 200 mg/kg metanil yellow, and three groups treated with metanil yellow plus2.5 mg·kg-1·day-1bees’ honey for 8 weeks. The obtained data showed that the antioxidant/anti-inflammatory activity of bees’ honey reduced the oxidative stress in the liver tissue and downregulated the inflammatory markers. In addition, the elevated levels of AGE and the activated NF-κB in the metanil-yellow-treated animals were significantly attenuated. Moreover, the levels of TNF-αand IL-1βwere significantly attenuated as a result of bees’ honey administration. Furthermore, the histopathological examination of the liver showed that bees’ honey reduced fatty degeneration, cytoplasmic vacuolization, and necrosis in metanil-yellow-treated rats. In conclusion, the obtained data suggest that bees’ honey has hepatoprotective effect on acute liver injuries induced by metanil-yellowin vivo, and the results suggested that the effect of bees’ honey against metanil yellow-induced liver damage is related to its antioxidant/anti-inflammatory properties which attenuate the activation of NF-κB and its controlled genes like TNF-αand IL-1β.

scholarly journals Possible Protective Effect of Silver Nanoparticles against Cisplatin Induced Pulmonary Inflammation in Rat Model

2021 ◽  
pp. 453-463
Author(s):  
Hanan Y. Alharbi ◽  
Nawal W. Helmi ◽  
Neveen A. Salem
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticles ◽  
Lung Tissue ◽  
Pulmonary Inflammation ◽  
Normal Lung ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Apoptotic Markers ◽  
Group I

Silver nanoparticles (AgNPs) are gaining interest in medical applications for their prominent antibacterial and antimicrobial potentials. AgNPs possess remarkable anti-inflammatory and antioxidant activities and enhances wound healing. The main objective of the current study was to investigate the therapeutic effect of administration of AgNPs on cisplatin (CP) induced pulmonary inflammation in rats. Sixty male albino rats were used in this study. Rats were divided into 6 groups (n=10). Group I control group. Group II and III control groups received AgNPs at doses (5 and 10 ppm). Group IV CP group received CP (2.5 mg/kg). Group V and VI CP group received AgNPs (5, and 10 ppm). All doses were administered intraperitoneally once a day for 4 weeks. Oxidative stress and antioxidant status, inflammatory mediators, fibrogenic as well as apoptotic markers were determined in lung tissues. The results revealed that rats treated with CP showed remarkable elevation in lung tissues MDA, TNF-α, IFN-γ, IL-6, CRP, Fibrinogen and P53 levels associated with depression in SOD, GSH and CAT activities. However, administration of AgNPs (5 or 10 ppm) to CP group resulted in significant amelioration of the aforementioned parameters in a dose dependent manner. Histopathological investigation of lung tissues of CP group demonstrated disruption of normal lung architecture and lung injury. However, treatment with AgNPs revealed significant improvement in lung tissue against CP- induced inflammatory changes and lung tissue damage. It could be concluded that AgNPs exert potent cytoprotective effects via combating oxidative stress, inflammation, fibrogenic and apoptotic markers and repairing histopathological changes in lung tissues.

scholarly journals Efektifitas Anti Inflamasi Formulasi Kunyit (Curcuma Longa), Daun Binahong (Anredera Cordifolia) Dan Daun Sambiloto (Andrographis Paniculata) Terhadap Luka Sayat Pada Kelinci

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Kh Endah Widhi Astuti ◽  
Sih Rini Handajani
Keyword(s):  
Curcuma Longa ◽  
Healing Process ◽  
Andrographis Paniculata ◽  
Control Group ◽  
Wound Healing Process ◽  
Second Stage Of Labor ◽  
Post Test ◽  
Treatment Research ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Background: Perineal lacerations are tears that occur in the perineum because of the second stage of labor. The Javanese people do the purpose of this study is the effectiveness of anti-inflammatory formulation of turmeric (curcuma longa), leaves of Binahong (Anredera cordifolia) and leaves of Sambiloto (Andrographis paniculata) against incisions in rabbits. Method: The method used in this study is the method in this study. This research uses a quantitative method with a type of pure treatment research in the form of pre-post test design. The sample in this study was 10 rabbits per group in 5 groups. Results: There was an anti-inflammatory effect of the turmeric formulation, binahong leaves of bitter leaf (F1, F2 and F3) in the incision wound in rabbits (ρ <0.001). There is an anti-inflammatory effect of turmeric formulation, binahong leaves of bitter leaf on leukocyte levels in rabbit incisions in F1, F2 and F3 compared to the control group (ρ <0.001). Conclusion: Turmeric formula, binahong leaf, bitter leaf (F1, F2 and F3) in the incision in rabbits can improve the wound healing process.

scholarly journals Effect of metformin gel against imiquimod–induced psoriasis in mice

2019 ◽  
Vol 10 (2) ◽  
pp. 795-802
Author(s):  
Haider F. Alsaedi ◽  
Haider Falih Shamikh Al-Saedi ◽  
Adeeb Ahmed Kadhim Al-Zubaidy ◽  
Mukhallad Abdul Kareem Ramadhan ◽  
Hussein Abdelamir Mohammad
Keyword(s):  
Biochemical Parameters ◽  
Negative Control Group ◽  
Negative Control ◽  
Tissue Homogenate ◽  
Control Group ◽  
Fourth Group ◽  
Inflammatory Parameters ◽  
Induction Group ◽  
Ointment Base ◽  
Anti Inflammatory

Imiquimod-induced psoriasis is an animal model of psoriasis.  The antidiabetic metformin had anti-inflammatory, immunomodulation, antiangiogenesis and antiproliferative effect. The objective of this study was to evaluate the possible beneficial effect of metformin gel against imiquimod-induced psoriasis-like form by scoring the erythema and scaling besides measuring of tissue homogenate levels of IL17/IL23. Swiss albino mice were used in this experiment imiquimod 5% cream applied on shaved back of mice for six days, and this is an induction group and compared with a negative control group that involved mice treated with ointment base only for six days. Third group; metformin 10% gel applied on the back of mice with imiquimod for six days. The fourth group included used of metformin 15% gel with imiquimod for six days. This data was analysed for significant level when p ≥ 0.05 by using either ANOVA test for biochemical parameters levels evaluation.    Furthermore, imiquimod 5% cream of induction group caused an elevation of inflammatory parameters; IL17 and IL23 in skin tissue homogenate when being compared to   IL17   and IL23 level of the control group. Whereas, metformin 10% gels showed levels of IL17 and IL23 with significant different from induction group. In addition, metformin 15% gels exerted a significantly low level of IL17 and IL23. The possible considerable antipsoriatic activity of topical metformin gel through reducing scaling and erythema and mediated through modest anti-inflammatory effects by suppressing levels of IL17/23.

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