scholarly journals Serodiagnosis of tuberculous lymphadenitis using a combination of antigens

2010 ◽  
Vol 4 (02) ◽  
pp. 096-102 ◽  
Author(s):  
Demissew Beyene ◽  
Kees Lumc Franken ◽  
Lawrence Yamuah ◽  
Abraham Aseffa ◽  
Harald G Wiker ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Serological Test ◽  
Invasive Procedure ◽  
Antibody Detection ◽  
Tuberculous Lymphadenitis ◽  
Igg Antibody ◽  
Extra Pulmonary Tuberculosis ◽  
Combined Use ◽  
Specific Igg ◽  
Low Sensitivity

Background: The diagnosis of extra-pulmonary tuberculosis (EPTB) by conventional methods such as culture and microscopy has low sensitivity and requires an invasive procedure. A simple rapid serological test would be of great value. Methodology: Six antigens (ESAT-6, Ag85A, TB10.4, Rv3881c, lipoarabinomannan (LAM) and Ara6-BSA) were tested in an ELISA to detect antigen-specific IgG and IgM antibodies in sera from 54 culture- and histology-confirmed tuberculous lymphadenitis (TBLN) patients as follows: four were HIV seropositive; sera from 25 was smear positive for pulmonary tuberculosis (PTB); 15 were culture- and histology-negative lymphadenitis (non-TBLN) patients; and 22 werehealthy controls (HCs). Results: The sensitivities of the antigens for the detection of IgG in sera of TBLN patients ranged from 4% to 30%. Specificities ranged from 73% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. Sensitivities of the antigens for detection of IgM ranged from 0% to 15% and specificities ranged from 80% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. LAM was the most potent antigen for detection of IgG. When LAM and ESAT-6 were combined, sensitivity increased up to 43% and specificity with non-TBLN was 80% with HC 96%. Conclusions: The study suggests that the combined use of LAM and ESAT-6 for IgG antibody detection in sera from TBLN patients could be a supplement to microscopy of fine-needle aspirate (FNA) to diagnose TBLN among patients suspected of TBLN.

CORRELATION OF CYTOMORPHOLOGY WITH ZN AND AR POSITIVITY ON FNAC IN THE DIAGNOSIS OF EXTRA PULMONARY TUBERCULOSIS

2021 ◽  
pp. 7-8
Author(s):  
Sana Umar ◽  
Syed Shamshad Ahmad ◽  
Kafil Akhtar ◽  
Zuber Ahmad
Keyword(s):  
Pulmonary Tuberculosis ◽  
Diagnostic Yield ◽  
Extrapulmonary Tuberculosis ◽  
Invasive Procedure ◽  
Epithelioid Cell ◽  
Chi Square ◽  
Extra Pulmonary Tuberculosis ◽  
Epithelioid Cell Granuloma ◽  
Extra Pulmonary ◽  
Low Sensitivity

BACKGROUND: FNAC is a minimally invasive procedure with a signicant diagnostic role in extrapulmonary tuberculosis. ZN Stain for demonstration of mycobacterium tuberculosis is extensively used. However, it has low sensitivity. Fluorochrome stain like Auramine- Rhodamine (AR) appear to be more likely to detect tubercular bacilli than ZN stain and also reduces the examination time. To study the OBJECTIVES: correlation of cytomorphology of extrapulmonary tuberculosis(EPTB) obtained on FNAC with ZN and AR positivity. MATERIALMETHODS: A total of 250 patients were taken, that were referred to the Department of Pathology, Jawaharlal Nehru Medical College, AMU, Aligarh from October 2015 to November 2017. Samples were collected by FNA and smears prepared were stained with H&E, ZN and AR stain. Smears were observed for positivity of Acid Fast Bacilli (AFB). Culture was taken as gold standard. RESULTS: The most common site of extra pulmonary tuberculosis was cervical lymph node seen in 76.4% cases and the most common cytomorphological pattern on FNAC was epithelioid cell granuloma with necrosis seen in 86.4% cases. The sensitivity of AR stain in picking up AFB was found to be 63.4% and the specicity was 81.9%, whereas ZN stain had a low sensitivity of 45.3% but had a high specicity of 87.9%. Statistically signicant difference between the two stains was seen on applying chi square test (p<0.001). Cohen's kappa coefcient for ZN vs AR stain was 0.65 and the strength of agreement between the two stains was substantial CONCLUSION: AR stain is more sensitive than ZN stain in diagnosing extrapulmonary tuberculosis and on combining it with cytomorphology it can help increase the diagnostic yield.

scholarly journals Analysis of the relations between allergen specific LgG antibody and allergic dermatosis of 14 kinds foods

Open Medicine ◽  
2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Hu Yin’e ◽  
Dai Shufang ◽  
Wang Bin ◽  
Qu Wei ◽  
Gao Junling ◽  
...  
Keyword(s):  
Food Intolerance ◽  
Antibody Detection ◽  
Igg Antibody ◽  
Allergic Dermatitis ◽  
Positive Antibody ◽  
Significant Difference ◽  
High Incidence ◽  
Positive Rate ◽  
High Incidence Rate ◽  
Specific Igg

AbstractTo use food-specific IgG antibody detection to explore its application in the allergy dermatoses. 181 patients were included from January 2014 to September 2014. Fourteen food-specific IgG antibodies were detected by ELISA. The positive rates of IgG antibody of the patient group and the healthy group were significantly different. The positive rates of IgG antibody of egg, milk, shrimp and crab took a large proportion in three groups of patients with three kinds of allergy dermatoses of urticaria, eczema and allergic dermatitis, the proportion of which was respectively 70.2%, 77.8% and 71.7%. There was mild and moderate intolerance of food in the allergic dermatitis group while there was no distribution difference of food intolerance in urticaria group and eczema group. Among urticaria and allergic dermatitis patients with positive antibody, the positive rate of children was significantly higher than that of adults while there was no significant difference between children and adults among eczema patients with positive antibody. Allergy dermatoses are closely related to food-specific IgG antibody and the allergy dermatoses patients have a high incidence rate of food intolerance; detecting IgG antibody in patients is of great significance for the diagnosis and treatment of allergy dermatoses.

scholarly journals Retraction of: application of food-specific IgG antibody detection in allergy dermatosis

Open Medicine ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. 237
Author(s):  
Hu Yine ◽  
Dai Shufang ◽  
Wang Bin ◽  
Qu Wei ◽  
Muhammad Aqeel Ashraf ◽  
...  
Keyword(s):  
Antibody Detection ◽  
Igg Antibody ◽  
Specific Igg

scholarly journals Preliminary Results of Seroprevalence of SARS-CoV-2 at Community Clinics in Tokyo

2020 ◽  
Author(s):  
Morihito Takita ◽  
Tomoko Matsumura ◽  
Kana Yamamoto ◽  
Erika Yamashita ◽  
Kazutaka Hosoda ◽  
...  
Keyword(s):  
Point Of Care ◽  
Cross Reactivity ◽  
Igg Antibody ◽  
Community Clinics ◽  
Diagnostic Pcr ◽  
Point Of Care Test ◽  
Positive Rate ◽  
Specific Igg ◽  
Novel Coronavirus ◽  
Low Sensitivity

AbstractSerological evaluation with SARS-CoV-2 specific IgG antibody will be an alternative way to know the pandemic of novel coronavirus disease (COVID-19) if the capacity for diagnostic PCR test is limited. The point-of-care test to detect SARS-CoV-2 specific IgG antibody in peripheral blood (n =202) was performed in two community clinics in Tokyo, Japan. The overall positive rate of SRAS-CoV-2 IgG antibody was 5.9% (95% confidence interval[CI]: 3.1-10.1). Higher rate was observed for healthcare workers (n =55, 9.1 [3.0-20.0]). The limitation on antibody tests includes low sensitivity and potent cross-reactivity with the previous coronavirus. Robust healthcare policy to efficiently monitor COVID-19 spread is warranted in Tokyo.

scholarly journals Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for IgG antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay

2020 ◽  
Author(s):  
Joachim Marien ◽  
Johan Michiels ◽  
Leo Heyndrickx ◽  
Karen Kerkhof ◽  
Nikki Foque ◽  
...  
Keyword(s):  
Large Scale ◽  
Detection Threshold ◽  
Neutralizing Antibody ◽  
Cost Effective ◽  
Antibody Detection ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Serological Tests ◽  
Specific Igg ◽  
Antibody Levels

Large-scale serosurveillance of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) will only be possible if serological tests are sufficiently reliable, rapid and inexpensive. Current assays are either labour-intensive and require specialised facilities (e.g. virus neutralization assays), or expensive with suboptimal specificity (e.g. commercial ELISAs). Bead-based assays offer a cost-effective alternative and allow for multiplexing to test for antibodies of other pathogens. Here, we compare the performance of four antigens for the detection of SARS-CoV-2 specific IgG antibodies in a panel of sera that includes both severe (n=40) and mild (n=52) cases, using a neutralization and a Luminex bead-based assay. While we show that neutralising antibody levels are significantly lower in mild than in severe cases, we demonstrate that a combination of recombinant nucleocapsid protein (NP), receptor-binding domain (RBD) and the whole spike protein (S1S2) results in a highly sensitive (96%) and specific (99%) bead-based assay that can detect IgG antibodies in both groups. Although S1-specific IgG levels correlate most strongly with neutralizing antibody levels, they fall below the detection threshold in 10% of the cases in our Luminex assay. In conclusion, our data supports the use of RBD, NP and S1S2 for the development of SARS-CoV-2 serological bead-based assays. Finally, we argue that low antibody levels in mild/asymptomatic cases might complicate the epidemiological assessment of large-scale surveillance studies.

scholarly journals Potential False-Positive and False-Negative Results for COVID-19 IgG/IgM Antibody Testing After Heat-Inactivation

2021 ◽  
Vol 7 ◽  
Author(s):  
Jie Lin ◽  
Wei Dai ◽  
Weiwei Li ◽  
Li Xiao ◽  
Tao Luo ◽  
...  
Keyword(s):  
False Negative ◽  
Antibody Detection ◽  
Heat Inactivation ◽  
Antibody Testing ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Negative Results ◽  
False Negative Results ◽  
Specific Igg ◽  
Potential False Positive

Objectives: With the worldwide spread of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), various antibody detection kits have been developed to test for SARS-CoV-2– specific IgG, IgM, and total antibody. However, the use of different testing methods under various heat-inactivation conditions might affect the COVID-19 detection results.Methods: Seven different antibody detection kits produced by four manufacturers for detection of SARS-CoV-2 IgG, IgM, and total antibody were tested at Wuhan Huoshenshan Hospital, China. Most of the kits used the indirect immunity, capture, and double-antigen sandwich methods. The effects of various heat-inactivation conditions on SARS-CoV-2-specific IgG, IgM, and total antibody detection were analyzed for the different test methods.Results: Using the indirect immunity method, values for SARS-CoV-2 IgG antibody significantly increased and those for IgM antibody decreased with increasing temperature of heat-inactivation using indirect immunity method. However, values for SARS-CoV-2 IgM and total antibody showed no change when the capture and double-antigen sandwich methods were used. The changes in IgG and IgM antibody values with the indirect immunity method indicated that heat-inactivation could affect COVID-19 detection results obtained using this method. In particular, 18 (22.2%) SARS-CoV-2 IgM positive samples were detected as negative with heat-inactivation at 65°C for 30 min, and one (25%) IgG negative sample was detected as positive after heat-inactivation at 56°C for 60 min and 60°C for 30 min.Conclusions: Heat-inactivation could increase SARS-CoV-2 IgG antibody values, and decrease IgM antibody values, causing potential false-positive or false-negative results for COVID-19 antibody detection using the indirect immunity method. Thus, before conducting antibody testing, the testing platforms should be evaluated in accordance with the relevant requirements to ensure accurate COVID-19 detection results.

scholarly journals Application of Food-specific IgG Antibody Detection in Allergy Dermatosis

Open Medicine ◽  
2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Hu Yine ◽  
Dai Shufang ◽  
Wang Bin ◽  
Qu Wei ◽  
Muhammad Aqeel Ashraf ◽  
...  
Keyword(s):  
Antibody Detection ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Allergic Dermatitis ◽  
Positive Antibody ◽  
Significant Difference ◽  
Positive Rate ◽  
High Incidence Rate ◽  
Specific Igg ◽  
Specific Igg Antibodies

AbstractThe application of food-specific IgG antibody detection in allergy dermatoses was explored. 181 patients with allergy dermatoses were diagnosed from January to September 2014 and 20 healthy subjects were selected. Fourteen kinds of food-specific IgG antibodies were detected by ELISA method among all the subjects. The positive rates of IgG antibody of the patient group and the healthy group were respectively 65.2% and 5.0%. The positive rates of IgG antibody of egg, milk, shrimp and crab took a large proportion in three groups of patients with three kinds of allergy dermatoses of urticaria, eczema and allergic dermatitis, the proportion of which was respectively 70.2%, 77.8% and 71.7%. Among urticaria and allergic dermatitis patients with positive antibody, the positive rate of children was significantly higher than that of adults (p<0.05) while there was no significant difference between children and adults among eczema patients with positive antibody (p>0.05). Allergy dermatoses are closely related to food-specific IgG antibodies, and the allergy dermatoses patients have a high incidence rate of food intolerance; detecting IgG antibody in the serum of patients is of great significance for the diagnosis and treatment of allergy dermatoses.

The Inducing Roles of the New Isolated Bursal Hexapeptide and Pentapeptide on the Immune Response of AIV Vaccine in Mice

2019 ◽  
Vol 26 (7) ◽  
pp. 542-549 ◽  
Author(s):  
Shan Shan Hao ◽  
Man Man Zong ◽  
Ze Zhang ◽  
Jia Xi Cai ◽  
Yang Zheng ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cells ◽  
Amino Acid ◽  
T Cell ◽  
Antigen Presentation ◽  
Amino Acid Sequences ◽  
Cell Subpopulation ◽  
Igg Antibody ◽  
Antibody Titers ◽  
Specific Igg

Background: Bursa of Fabricius is the acknowledged central humoral immune organ. The bursal-derived peptides play the important roles on the immature B cell development and antibody production. Objective: Here we explored the functions of the new isolated bursal hexapeptide and pentapeptide on the humoral, cellular immune response and antigen presentation to Avian Influenza Virus (AIV) vaccine in mice immunization. Methods: The bursa extract samples were purified following RP HPLC method, and were analyzed with MS/MS to identify the amino acid sequences. Mice were twice subcutaneously injected with AIV inactivated vaccine plus with two new isolated bursal peptides at three dosages, respectively. On two weeks after the second immunization, sera samples were collected from the immunized mice to measure AIV-specific IgG antibody levels and HI antibody titers. Also, on 7th day after the second immunization, lymphocytes were isolated from the immunized mice to detect T cell subtype and lymphocyte viabilities, and the expressions of co-stimulatory molecule on dendritic cells in the immunized mice. Results: Two new bursal hexapeptide and pentapeptide with amino acid sequences KGNRVY and MPPTH were isolated, respectively. Our investigation proved the strong regulatory roles of bursal hexapeptide on AIV-specific IgG levels and HI antibody titers, and lymphocyte viabilities, and the significant increased T cells subpopulation and expressions of MHCII molecule on dendritic cells in the immunized mice. Moreover, our findings verified the significantly enhanced AIV-specific IgG antibody and HI titers, and the strong increased T cell subpopulation and expressions of CD40 molecule on dendritic cells in the mice immunized with AIV vaccine and bursal pentapeptide. Conclusion: We isolated and identified two new hexapeptide and pentapeptide from bursa, and proved that these two bursal peptides effectively induced the AIV-specific antibody, T cell and antigen presentation immune responses, which provided an experimental basis for the further clinical application of the bursal derived active peptide on the vaccine improvement.

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