Application of the Apn2/MAT locus to improve the systematics of the Colletotrichum gloeosporioides complex: an example from coffee (Coffea spp.) hosts

Mycologia ◽  
2012 ◽  
Vol 104 (2) ◽  
pp. 396-409 ◽  
Author(s):  
Diogo Nuno Silva ◽  
Pedro Talhinhas ◽  
Vítor Várzea ◽  
Lei Cai ◽  
Octávio Salgueiro Paulo ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Gloeosporioides Complex ◽  
Mat Locus

scholarly journals Genetic Diversity of Colletotrichum spp. Causing Strawberry Anthracnose in Zhejiang, China

Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1351-1357 ◽  
Author(s):  
X. Y. Chen ◽  
D. J. Dai ◽  
S. F. Zhao ◽  
Y. Shen ◽  
H. D. Wang ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Dominant Species ◽  
Colletotrichum Gloeosporioides ◽  
Morphological Characteristics ◽  
Zhejiang Province ◽  
Control Measures ◽  
Chitin Synthase ◽  
Colletotrichum Gloeosporioides Complex ◽  
Strawberry Anthracnose ◽  
Colletotrichum Spp

Anthracnose is a serious fungal disease that primarily infects strawberry roots and stolons during development. Here, 91 isolates from different areas of Zhejiang province, China, were collected. Morphological characteristics were analyzed, and a phylogenetic analysis based on multiple genes (actin, internal transcribed spacer, calmodulin, glyceraldehyde-3-phosphate dehydrogenase, and chitin synthase) was performed. We found that all of the Colletotrichum species causing strawberry anthracnose belonged to the Colletotrichum gloeosporioides complex. Among them, we identified 48 isolates of C. fructicola, 21 isolates of C. siamense, 13 isolates of C. gloeosporioides, and 9 isolates of C. aenigma. C. siamense was distributed in the central and eastern regions of Zhejiang province (Hangzhou, Jinhua, Shaoxing, Ningbo, and Taizhou). This is the first report of C. siamense causing strawberry anthracnose in Zhejiang province. C. fructicola was the most dominant species causing strawberry anthracnose in Zhejiang province. We identified the four species causing strawberry anthracnose in Zhejiang province, which will improve our understanding of the strawberry anthracnose epidemic and will benefit the development of future control measures.

scholarly journals Molecular and Morphological Characterization of Colletotrichum Species in the Colletotrichum gloeosporioides Complex Associated with Persimmon Anthracnose in South Korea

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 1015-1024 ◽  
Author(s):  
Oliul Hassan ◽  
Jong Yeob Jeon ◽  
Taehyun Chang ◽  
Jun Sung Shin ◽  
Nam Kwon Oh ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
South Korea ◽  
Colletotrichum Gloeosporioides ◽  
Morphological Characteristics ◽  
Intergenic Spacer ◽  
Morphological Characterization ◽  
Persimmon Fruit ◽  
Colletotrichum Gloeosporioides Complex ◽  
Mating Type Genes

Anthracnose is a major disease of persimmon in the pre- and postharvest phase. Several species of Colletotrichum (Colletotrichum gloeosporioides, C. acutatum, and C. horii) have been reported as causal agents of persimmon anthracnose in South Korea. In this study, a collection of 50 isolates associated with persimmon anthracnose were collected from Sangju (n = 25) and Cheongdo-gun (n = 25), South Korea. The morphological characteristics of all 50 Colletotrichum isolates were similar, and it was difficult to identify the isolates to the species level. A subsample of eight isolates was characterized phylogenetically to ascertain species. BLAST search and phylogenetic analysis of the internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and actin (ACT) genes revealed two species: C. horii as well as a previously unreported persimmon anthracnose causal agent C. siamense. C. siamense isolates were confirmed again by phylogenetic analysis of the ITS, ACT, GAPDH, calmodulin, and Apn2-Mat1-2 intergenic spacer partial mating type genes. Koch’s postulates for C. horii and C. siamense were fulfilled, confirming the pathogenicity of the two species in persimmon fruit. Morphological characteristics (colony morphology and size and shape of conidia and appressoria) from two representative isolates support results of the phylogenetic analysis and match those of previous descriptions of C. horii and C. siamense.

Colletotrichum Gloeosporioides Species Complex: Pathogen Causing Anthracnose, Gummosis and Die-Back Diseases of Cashew (Anacardium Occidentale L.) In Ghana

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
A. Muntala ◽  
P. M. Norshie ◽  
K. G. Santo ◽  
C. K. S. Saba
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Potato Dextrose Agar ◽  
Causal Agent ◽  
Anacardium Occidentale ◽  
Causative Organism ◽  
Pathogenicity Test ◽  
Small Water ◽  
Colletotrichum Gloeosporioides Species Complex

A survey was conducted in twenty-five cashew (Anacardium occidentale) orchards in five communities in the Dormaa-Central Municipality of Bono Region of Ghana to assess the incidence and severity of anthracnose, gummosis and die-back diseases on cashew. Cashew diseased samples of leaves, stem, inflorescences, twigs, flowers, nuts and apples showing symptoms (e. g. small, water-soaked, circular or irregular yellow, dark or brown spots or lesions on leaves, fruits and flowers, sunken surface, especially on the apples, blight, gum exudates) were collected for isolation of presumptive causative organism. The pathogen was isolated after disinfecting the excised diseased pieces in 70% ethanol, plated on potato dextrose agar (PDA) and incubated at 28 oC for 3 to 7 days. The identity of the putative pathogen was morphologically and culturally confirmed as belonging to Colletotrichum gloeosporioides species complex using standard mycological identification protocols. The pathogen had varied conidia sizes of between 9-15 up to 20 μm in length and diameter of 3-6 μm. The conidia were straight and cylindrically shaped with rounded or obtuse ends. The septate mycelium was whitish-grey, velvety and cotton-like in appearance from the top. The results confirmed the presence of the pathogen in the orchards with incidence ranging from 6.9% and 14.0% for gummosis and averaged 22.9% for anthracnose infected orchards. The result of the pathogenicity test confirmed the isolates to be pathogenic on inoculated cashew seedlings and were consistently re-isolated, thereby establishing the pathogen as the true causal agent of the said diseases in cashew trees and thus completed the Koch’s postulate.

METODOLOGIA DE INOCULAÇÃO DE Colletotrichum gloeosporioides EM FRUTOS DE MAMÃO

2012 ◽  
Vol 24 (3) ◽  
pp. 183-188
Author(s):  
Lahyre Izaete Silveira Gomes ◽  
Eduardo Alves ◽  
Enilton Nascimento de Santana ◽  
Hilário Antônio de Castro
Keyword(s):  
Colletotrichum Gloeosporioides

Control of Winged Waterprimrose (Jussiaea decurrens) and Northern Jointvetch (Aeschynomene virginica) with Fungal Pathogens

Weed Science ◽  
1979 ◽  
Vol 27 (5) ◽  
pp. 497-501 ◽  
Author(s):  
C. D. Boyette ◽  
G. E. Templeton ◽  
R. J. Smith
Keyword(s):  
Oryza Sativa ◽  
Glycine Max ◽  
Fungal Pathogens ◽  
Liquid Culture ◽  
Field Experiments ◽  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungus ◽  
Field Tests ◽  
Wide Range ◽  
Growing Region

An indigenous, host-specific, pathogenic fungus that parasitizes winged waterprimrose [Jussiaea decurrens(Walt.) DC.] is endemic in the rice growing region of Arkansas. The fungus was isolated and identified asColletotrichum gloeosporioides(Penz.) Sacc. f.sp. jussiaeae(CGJ). It is highly specific for parasitism of winged waterprimrose and not parasitic on creeping waterprimrose (J. repensL. var.glabrescensKtze.), rice (Oryza sativaL.), soybeans [Glycine max(L.) Merr.], cotton (Gossypium hirsutumL.), or 4 other crops and 13 other weeds. The fungus was physiologically distinct from C.gloeosporioides(Penz.) Sacc. f. sp.aeschynomene(CGA), an endemic anthracnose pathogen of northern jointvetch[Aeschynomene virginica(L.) B.S.P.], as indicated by cross inoculations of both weeds. Culture in the laboratory and inoculation of winged waterprimrose in greenhouse, growth chamber and field experiments indicated that the pathogen was stable, specific, and virulent in a wide range of environments. The pathogen yielded large quantities of spores in liquid culture. It is suitable for control of winged waterprimrose. Winged waterprimrose and northern jointvetch were controlled in greenhouse and field tests by application of spore mixtures of CGJ and CGA at concentrations of 1 to 2 million spores/ml of each fungus in 94 L/ha of water; the fungi did not damage rice or nontarget crops.

Sign in / Sign up

Export Citation Format

Share Document