scholarly journals Small Molecule Pan-Dengue and West Nile Virus NS3 Protease Inhibitors

2011 ◽  
Vol 21 (5) ◽  
pp. 209-217 ◽  
Author(s):  
Lynne Cregar-Hernandez ◽  
Guan-Sheng Jiao ◽  
Alan T Johnson ◽  
Axel T Lehrer ◽  
Teri Ann S Wong ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Protease Inhibitors ◽  
Small Molecule ◽  
West Nile ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease

Exploring the binding of peptidic West Nile virus NS2B–NS3 protease inhibitors by NMR

2013 ◽  
Vol 97 (2) ◽  
pp. 137-144 ◽  
Author(s):  
CongBao Kang ◽  
Shovanlal Gayen ◽  
Weiling Wang ◽  
Rene Severin ◽  
Angela Shuyi Chen ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Protease Inhibitors ◽  
West Nile ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease

A FRET-based assay for the discovery of West Nile Virus NS2B-NS3 protease inhibitors

2013 ◽  
Vol 23 (17) ◽  
pp. 4848-4850 ◽  
Author(s):  
Rebecca N. Adamek ◽  
Roxanne V. Maniquis ◽  
Sabaha Khakoo ◽  
Michael D. Bridges ◽  
Nicholas T. Salzameda
Keyword(s):  
West Nile Virus ◽  
Protease Inhibitors ◽  
West Nile ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease

scholarly journals Structure‐Based Macrocyclization of Substrate Analogue NS2B‐NS3 Protease Inhibitors of Zika, West Nile and Dengue viruses

ChemMedChem ◽  
2020 ◽  
Vol 15 (15) ◽  
pp. 1439-1452 ◽  
Author(s):  
Niklas J. Braun ◽  
Jun P. Quek ◽  
Simon Huber ◽  
Jenny Kouretova ◽  
Dorothee Rogge ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
West Nile ◽  
Dengue Viruses ◽  
Substrate Analogue ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease

scholarly journals Potential Dual Role of West Nile Virus NS2B in Orchestrating NS3 Enzymatic Activity in Viral Replication

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 216
Author(s):  
Alanna C. Tseng ◽  
Vivek R. Nerurkar ◽  
Kabi R. Neupane ◽  
Helmut Kae ◽  
Pakieli H. Kaufusi
Keyword(s):  
West Nile Virus ◽  
Enzymatic Activity ◽  
Nonstructural Protein ◽  
Antiviral Drug ◽  
West Nile ◽  
Biochemical Assays ◽  
In Trans ◽  
Viral Polyprotein ◽  
Ns3 Protease

West Nile virus (WNV) nonstructural protein 3 (NS3) harbors the viral triphosphatase and helicase for viral RNA synthesis and, together with NS2B, constitutes the protease responsible for polyprotein processing. NS3 is a soluble protein, but it is localized to specialized compartments at the rough endoplasmic reticulum (RER), where its enzymatic functions are essential for virus replication. However, the mechanistic details behind the recruitment of NS3 from the cytoplasm to the RER have not yet been fully elucidated. In this study, we employed immunofluorescence and biochemical assays to demonstrate that NS3, when expressed individually and when cleaved from the viral polyprotein, is localized exclusively to the cytoplasm. Furthermore, NS3 appeared to be peripherally recruited to the RER and proteolytically active when NS2B was provided in trans. Thus, we provide evidence for a potential additional role for NS2B in not only serving as the cofactor for the NS3 protease, but also in recruiting NS3 from the cytoplasm to the RER for proper enzymatic activity. Results from our study suggest that targeting the interaction between NS2B and NS3 in disrupting the NS3 ER localization may be an attractive avenue for antiviral drug discovery.

Flavonoids from Pterogyne nitens as Zika virus NS2B-NS3 protease inhibitors

2021 ◽  
Vol 109 ◽  
pp. 104719
Author(s):  
Caroline Sprengel Lima ◽  
Melina Mottin ◽  
Leticia Ribeiro de Assis ◽  
Nathalya Cristina de Moraes Roso Mesquita ◽  
Bruna Katiele de Paula Sousa ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Zika Virus ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease

Phenylglycine as a novel P2 scaffold in hepatitis C virus NS3 protease inhibitors

2007 ◽  
Vol 15 (3) ◽  
pp. 1448-1474 ◽  
Author(s):  
Pernilla Örtqvist ◽  
Shane D. Peterson ◽  
Eva Åkerblom ◽  
Thomas Gossas ◽  
Yogesh A. Sabnis ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protease Inhibitors ◽  
Ns3 Protease Inhibitors ◽  
Ns3 Protease
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