scholarly journals Evaluation of the Immunomodulatory Properties of a Calf Lipid Thymus Extract and Standardization of a Method for Quantification of its Biological Activity

2007 ◽  
Vol 3 (2) ◽  
pp. 35-44
Author(s):  
Jasna Novakovic ◽  
Miodrag Colic ◽  
Radmila Popovic ◽  
Dusanka Mrdakovic ◽  
Milena Radeta ◽  
...  
Keyword(s):  
Biological Activity ◽  
Thymus Extract ◽  
Immunomodulatory Properties

scholarly journals Titanium Surface Properties Influence the Biological Activity and FasL Expression of Craniofacial Stromal Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Enrico Conserva ◽  
Alessandra Pisciotta ◽  
Francesco Borghi ◽  
Milena Nasi ◽  
Simone Pecorini ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Biological Activity ◽  
Surface Properties ◽  
Stromal Cells ◽  
Titanium Surface ◽  
Growth Patterns ◽  
Immunomodulatory Activity ◽  
Fasl Expression ◽  
Osteogenic Markers ◽  
Immunomodulatory Properties

Mesenchymal stromal cells (MSCs) can be easily isolated form craniofacial bones during routine dentistry procedures. Due to their embryological origin from neural crest, they represent a suitable cell population to study cell-biomaterial interaction in the craniofacial field, including osteoinductive/osteointegrative processes. The biological and immunomodulatory properties of MSCs may be influenced by chemistry and topography of implant surfaces. We investigated if and how three different titanium surfaces, machined (MCH), sandblasted with resorbable blasting medium (RBM), and Ca++-nanostructured (NCA), may affect biological activity, osseointegration, and immunomodulatory properties of craniofacial MSCs. Cell proliferation, morphology, osteogenic markers, and FasL were evaluated on MSCs isolated from the mandibular bone after seeding on these three different surfaces. No statistically significant differences in cell proliferation were observed whereas different morphologies and growth patterns were detected for each type of surface. No difference in the expression of osteogenic markers was revealed. Interestingly, FasL expression, involved in the immunomodulatory activity of stem cells, was influenced by surface properties. Particularly, immunofluorescence analysis indicated that FasL expression increased on MCH surface compared to the others confirming the suggested role of FasL in promoting osteogenic differentiation. Titanium surface treatments and topography might reflect different biological behaviours of craniofacial MSCs and influence their osseointegration/immunomodulation properties.

scholarly journals Isolation of thymus gland fractions and the determination of their biological activity

2007 ◽  
Vol 72 (3) ◽  
pp. 225-234
Author(s):  
Milena Radeta ◽  
Jasna Novakovic ◽  
Milanka Vico-Stevanovic ◽  
Slobodanka Simic ◽  
Aleksandar Pirozkov
Keyword(s):  
Biological Activity ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Immunological Response ◽  
Hplc Method ◽  
Thymus Extract ◽  
Folch Method ◽  
Lipid Fractions ◽  
Calf Thymus

A calf thymus extract was prepared and fractionated into lipid and non-lipid fractions. The non-lipid fraction was isolated from the calf thymus extract using the Folch method. The components isolated from the non-lipid fraction were characterized by IR, NMR, biuret and HPLC method. The results of the analyses indicated the presence of peptides. The lipid fraction contained phospholipids, glycolipids and neutral lipids. The biological activity of both the isolated lipid and peptide fractions was determined by the in vivo hemolytic plaques method in Wistar rats with an involuted thymus. The peptide and phospholipid fractions of the thymus extract showed a significant increase of hemolytic plaques. The glycolipid and neutral lipid fraction failed to express a significant immunological response.

Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

1973 ◽  
Vol 31 ◽  
pp. 378-379
Author(s):  
G. Kasnic ◽  
S. E. Stewart ◽  
C. Urbanski
Keyword(s):  
Breast Cancer ◽  
Biological Activity ◽  
Human Breast Cancer ◽  
Osteogenic Sarcoma ◽  
Human Tumor ◽  
Structural Similarity ◽  
Cell Tumor ◽  
Human Breast ◽  
Human Tumor Cell ◽  
Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

Isothiazol-3(2 H )-ones, Part I: Synthesis, Reactions and Biological Activity

2002 ◽  
Vol 23 (1) ◽  
pp. 79-121 ◽  
Author(s):  
Kathleen Taubert ◽  
Susanne Kraus ◽  
Bärbel Schulze
Keyword(s):  
Biological Activity ◽  
Synthesis Reactions

Evaluation of biological activity of lignans extracted from tree knots

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
E Spilioti ◽  
B Holmbom ◽  
P Moutsatsou
Keyword(s):  
Biological Activity

Effect of the HDAC inhibitor SAHA on the immunomodulatory properties of MSC and tumor stroma cells

2010 ◽  
Vol 222 (03) ◽  
Author(s):  
A Kruchen ◽  
PD Johann ◽  
R Handgretinger ◽  
I Mueller
Keyword(s):  
Hdac Inhibitor ◽  
Tumor Stroma ◽  
Immunomodulatory Properties
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