Genetic Monitoring of Multi-Functional Plant Growth Promoting Rhizobacteria Bacillus subtilis AH18 and Bacillus licheniformis K11 by Multiplex and Real-Time Polymerase Chain Reaction in a Pepper Farming Field

2011 ◽  
Vol 54 (2) ◽  
Author(s):  
Jong-Hui Lim
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Real Time ◽  
Bacillus Licheniformis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Chain Reaction ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Polymerase Chain

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

scholarly journals Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group)

Vegetalika ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 512
Author(s):  
Nanda Dwi Hafri ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Allium Cepa ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Methylotrophicus ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

scholarly journals Effect of plant growth promoting rhizobacteria (PGPR) and IBA treatments on rooting in cuttings of apple (Malus × domestica Borkh.) clonal rootstock Merton 793

2017 ◽  
Vol 9 (2) ◽  
pp. 1135-1138
Author(s):  
Pramod Verma ◽  
P. S. Chauhan ◽  
J. S. Chandel
Keyword(s):  
Plant Growth ◽  
Bacillus Licheniformis ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Sp ◽  
Main Shoot ◽  
Primary Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Different Strains

The preliminary studies on the effect of different strains of plant growth promoting rhizobacteria (PGPR) alone and in combination with IBA at 1000 ppm on rooting in cuttings of apple clonal rootstock Merton 793 were carried out during 2012-13. The PGPR strains (RG (1)3 – Bacillus sp.), B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. alone failed to induce rooting response in cuttings of apple clonal rootstock Merton 793. The results revealed that IBA 2500 ppm recorded the maximum rooting (65 %), number of primary roots (5.00), length (28.43 cm) and diameter (3.25 mm) of primary roots, fresh (3.67 g) and dry weight (2.59 g) of roots, length of main shoot (134.14cm), diameter of main shoot (8.18 mm), fresh (30.40 g) and dry weight (22.60 g) of shoots in cuttings of Merton 793. However, the PGPR strains RG (1)3 – Bacillus sp., B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. in combination with IBA 1000 ppm showed improvement in rooting of cuttings to the extent of 10, 15 and 5 per cent rooting, respectively and growth of the rooted plants. IBA at 2500 ppm resulted better rooting and growth of rooted plants. Hence, this treatment is suggested for commercial propagation of apple clonal rootstock Merton 793 through cuttings.

The plant-growth-promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins

2001 ◽  
Vol 111 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Francisco Javier Gutiérrez-Mañero ◽  
Beatriz Ramos-Solano ◽  
Agustı´n Probanza ◽  
Jalel Mehouachi ◽  
Francisco R. Tadeo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Licheniformis ◽  
Bacillus Pumilus ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant Growth Enhancement using Rhizospheric Halotolerant Phosphate Solubilizing Bacterium Bacillus licheniformis QA1 and Enterobacter asburiae QF11 Isolated from Chenopodium quinoa Willd

2020 ◽  
Vol 8 (6) ◽  
pp. 948
Author(s):  
Ismail Mahdi ◽  
Nidal Fahsi ◽  
Mohamed Hafidi ◽  
Abdelmounaaim Allaoui ◽  
Latefa Biskri
Keyword(s):  
Plant Growth ◽  
Bacillus Licheniformis ◽  
Chenopodium Quinoa ◽  
Plant Growth Promoting Rhizobacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Saline Irrigation ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Enterobacter Asburiae

Plant growth-promoting rhizobacteria represent a promising solution to enhancing agricultural productivity. Here, we screened phosphate solubilizing bacteria from the rhizospheric soil of Chenopodium quinoa Willd and assessed their plant-growth promoting rhizobacteria (PGPR) properties including production of indole-3-acetic acid (IAA), siderophores, hydrogen cyanide (HCN), ammonia and extracellular enzymes. We also investigated their tolerance to salt stress and their capacity to form biofilms. Two isolated strains, named QA1 and QF11, solubilized phosphate up to 346 mg/L, produced IAA up to 795.31 µg/mL, and tolerated up to 2 M NaCl in vitro. 16S rRNA and Cpn60 gene sequencing revealed that QA1 and QF11 belong to the genus Bacillus licheniformis and Enterobacter asburiae, respectively. In vivo, early plant growth potential showed that quinoa seeds inoculated either with QA1 or QF11 displayed higher germination rates and increased seedling growth. Under saline irrigation conditions, QA1 enhanced plant development/growth. Inoculation with QA1 increased leaf chlorophyll content index, enhanced P and K+ uptake and decreased plant Na+ uptake. Likewise, plants inoculated with QF11 strain accumulated more K+ and had reduced Na+ content. Collectively, our findings support the use of QA1 and QF11 as potential biofertilizers.

scholarly journals Aplikasi Plant Growth Promoting Rhizobacteria dalam Meningkatkan Produktivitas Bawang Merah di Lahan Pasir Pantai

2019 ◽  
Vol 47 (1) ◽  
pp. 53-60
Author(s):  
Sumiyati Tuhuteru ◽  
Endang Sulistyaningsih ◽  
Dan Arif Wibowo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Burkholderia Cepacia ◽  
Block Design ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Methylotrophicus ◽  
Randomized Complete Block Design ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Coastal Land

The use of Plant Growth Promoting Rhizobacteria (PGPR) is one of the most effective techniques to improve fertility of sandy coastal lands. The purpose of this research was to obtain the most effective isolate of PGPR for the growth of three shallot cultivars in the sandy coastal land. The research was conducted in August-November 2015 at Samas sandy coastal land, Bantul. The two-factor treatments were assigned in a randomized complete block design with three replications. The first factor was shallot cultivars, consisted of Crok, Tiron, and Tuk-tuk, while the second factor was the PGPR suspension, i.e. control (without PGPR), PGPR BP25.2 isolate (Bacillus methylotrophicus), BP25.6 isolate (Bacillus amyloliquofaciens), BP25.7 isolate (Bacillus subtilis), BrSM 4 isolate (Burkholderia cepacia), and BrSG 5 isolate (Burkholderia seminalis). The results showed that all PGPR isolates tested had the ability to produce IAA, with the highest amount of IAA concentration produced by BrSG 5 isolate (Burkholderia seminalis) combined with the three cultivars (22.46 mg kg-1, 28.61 mg kg-1, 41.41 mg kg-1). BP25.2 isolate (Bacillus methylotrophicus) effectively induced N (0.05%), whereas BP25.7 isolate (Bacillus subtilis) effectively induced P (0.22 ppm).Keywords: Bacillus, bacteria, marginal land

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