Enhanced Production and Partial Purification of Glucoamylase from Mutated Bacillus sp. FME

2009 ◽  
Vol 52 (5) ◽  
pp. 412-418 ◽  
Author(s):  
Gudi Satheesh Kumar
Keyword(s):  
Partial Purification ◽  
Bacillus Sp ◽  
Enhanced Production

scholarly journals Enhanced production of nattokinase from UV mutated Bacillus sp.

2013 ◽  
Vol 8 (2) ◽  
Author(s):  
V Mohanasrinivasan ◽  
C Subathra Devi ◽  
Ritusree Biswas ◽  
Falguni Paul ◽  
Mohor Mitra ◽  
...  
Keyword(s):  
Bacillus Sp ◽  
Enhanced Production

Production and Thermal Characterization of an Alkaline Pectin Lyase from Penicillium notatum

2015 ◽  
Vol 11 (4) ◽  
pp. 517-525 ◽  
Author(s):  
Umme Habibah Siddiqua ◽  
Haq Nawaz Bhatti ◽  
Shazia Nouren ◽  
Saima Noreen ◽  
Ismat Bibi
Keyword(s):  
Ammonium Sulphate ◽  
Thermal Inactivation ◽  
Thermal Characterization ◽  
Maximal Activity ◽  
Inhibitory Effect ◽  
Pectin Lyase ◽  
Partial Purification ◽  
Penicillium Notatum ◽  
Enhanced Production ◽  
Lyase Activity

Abstract The present study was aimed to investigate the potential of Penicillium notatum for the production of pectin lyase under solid state culture using wheat bran as substrate. Different process parameters were optimized using completely randomized design for enhanced production of the pectin lyase. P. notatum showed maximum production (1875 U/gds) of pectin lyase with substrate amount 15 g/250 ml, moisture level 60%, pH 6, incubation period 120 h at 30°C. Pectin lyase activity was further improved with the addition of maltose and ammonium sulphate as carbon and nitrogen additives (1%), respectively. Partial purification of enzyme was carried out by ammonium sulphate precipitation at 80% saturation level. The P. notatum pectin lyase showed maximal activity at 65°C and pH 8. Km and Vmax values were 0.29% and 0.487 µmol/min, respectively. Energy of activation was found to be 5.33 kJ/mol. A detailed kinetic study of thermal inactivation was carried out. The results showed that pectin lyase exhibited resistance against thermal unfolding. Effect of various metals on pectin lyase activity was also investigated. All the metals showed inhibitory effect on the enzyme activity. The present investigation revealed that pectin lyase isolated from P. notatum is thermally stable and alkaline in nature.

Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches

2014 ◽  
Vol 38 (2) ◽  
pp. 299-305 ◽  
Author(s):  
In Yeong Kang ◽  
Jang Min Park ◽  
Won-Kyung Hong ◽  
Yun Seok Kim ◽  
You Ree Jung ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Bacillus Sp ◽  
Palm Fruit ◽  
Enhanced Production

scholarly journals Partial purification and sugarcane bagasse induction of extracellular thermostable Amylase by Bacillus sp. under submerged fermentation

2017 ◽  
Vol 1 (Special Issue) ◽  
pp. 156-156
Author(s):  
Shubhangi Padhi ◽  
Amrita Swain ◽  
Prachiprava Rout ◽  
Luna Samanta ◽  
Dhananjay Soren
Keyword(s):  
Sugarcane Bagasse ◽  
Submerged Fermentation ◽  
Partial Purification ◽  
Bacillus Sp ◽  
Thermostable Amylase
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