Isolation and Characterization of Circulating Tumor Cells in Patients with Metastatic Colorectal Cancer

2006 ◽  
Vol 6 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Steven J. Cohen ◽  
R. Katherine Alpaugh ◽  
Steve Gross ◽  
Shawn M. O'Hara ◽  
Denis A. Smirnov ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Isolation And Characterization

scholarly journals Molecular Characterization of Circulating Tumor Cells in Human Metastatic Colorectal Cancer

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e40476 ◽  
Author(s):  
Jorge Barbazán ◽  
Lorena Alonso-Alconada ◽  
Laura Muinelo-Romay ◽  
María Vieito ◽  
Alicia Abalo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Tumor Cells ◽  
Molecular Characterization ◽  
Circulating Tumor Cells

Morphologic and genomic characterization of circulating tumor cells in patients with metastatic colorectal cancer treated with combinational treatment of andecaliximab, bevacizumab and chemotherapy.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e14753-e14753
Author(s):  
Stephanie Shishido ◽  
Peter Kuhn
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Clinical Decision Making ◽  
Line Therapy ◽  
Combinational Treatment

e14753 Background: The liquid biopsy is a noninvasive route to evaluate circulating tumor cells (CTCs) during the course of treatment to gain understanding of tumor biology, with potential prognostic utility. CTCs could serve as a predictive biomarker while aiding in the identification of resistance mechanisms to treatment through single cell genomic and proteomic analysis providing a longitudinal snapshots of tumor heterogeneity. Methods: Through the use of the high definition single cell assay (HD-SCA) workflow, we characterized the rare circulating cells to determine prognostic value of the liquid biopsy in monitoring the efficacy of andecaliximab in a combinational treatment as 1st or 2nd line therapy in patients with metastatic colorectal cancer (mCRC). 174 samples from 95 patients were analyzed to determine the significance of CTCs during treatment. Results: HD-CTCs were detected in 31% of samples, with 41 (43%) patients being CTC positive in at least 1 timepoint during the study. Patients receiving 1st line therapy presented with a median of 0 (range 0-346.04) and a mean of 9.49 (±14.06) HD-CTC/mL at baseline (BL). At initiation of 2nd line therapy, patients presented with a median of 0 HD-CTC/mL (range 0-277.37) and a mean of 10.94 (±15.32). There was no association between BL HD-CTC/mL and response, but for the 20 patients with > 1 HD-CTC at BL, there was a trend toward response for higher HD-CTC/mL (non-response: mean 2.8, n = 12; response: mean 89.7, n = 8; p = .04). The 3 patients with > 10 HD-CTC/mL at BL had undetectable HD-CTC on-treatment, which accompanied radiologic partial response; however, the 2 patients with complete radiological response had no HD-CTC detected at BL. In case studies, treatment pressure led to an observable change in HD-CTC morphology and genomic instability (single cell CNV analysis), suggesting these parameters may inform prognosis. Conclusions: Characterization of CTCs from patients with mCRC is feasible and may provide prognostic information to guide clinical decision making. Further evaluation of CTCs for pharmacodynamics and clinical monitoring in patients with mCRC is warranted.

Abstract 2393: Isolation and characterization of circulating tumor cells in a mouse model of colorectal cancer

2012 ◽  
Author(s):  
Avisnata Das ◽  
Tiffany L. Whitcomb ◽  
Joshua E. Allen ◽  
David T. Dicker ◽  
Kristi L. Peters ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Isolation And Characterization

scholarly journals KRAS mutation analysis of single circulating tumor cells from patients with metastatic colorectal cancer

BMC Cancer ◽  
2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Yuurin Kondo ◽  
Kazuhiko Hayashi ◽  
Kazuyuki Kawakami ◽  
Yukari Miwa ◽  
Hiroshi Hayashi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Mutation Analysis ◽  
Kras Mutation

Isolation and characterization of circulating tumor cells using a novel workflow combining the CellSearch®system and the CellCelector™

2016 ◽  
Vol 33 (1) ◽  
pp. 125-132 ◽  
Author(s):  
Martin Horst Dieter Neumann ◽  
Helen Schneck ◽  
Yvonne Decker ◽  
Susanne Schömer ◽  
André Franken ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Isolation And Characterization
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