scholarly journals Evaluation and Comparison of the In Vitro Cytotoxic Activity of Withania somnifera Methanolic and Ethanolic Extracts against MDA-MB-231 and Vero Cell Lines

2016 ◽  
Vol 84 (1) ◽  
pp. 41-59 ◽  
Author(s):  
A. N. SRIVASTAVA
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Withania Somnifera ◽  
Ethanolic Extracts ◽  
Vero Cell Lines

Effect of Different Hydatid Cyst Molecules on Hela and Vero Cell Lines Growth In vitro

2013 ◽  
Vol 02 (01) ◽  
Author(s):  
Nasir Aref ◽  
Hedayatollah Shirzad ◽  
Morteza Yousefi ◽  
Hossein Yousofi.Darani
Keyword(s):  
Hydatid Cyst ◽  
Vero Cell ◽  
Cell Lines ◽  
Vero Cell Lines

scholarly journals Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum In Vitro: Effects on MCF-7 and Vero Cell Lines

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Faizah Bt. Harun ◽  
Syed Mohsin Syed Sahil Jamalullail ◽  
Khoo Boon Yin ◽  
Zulkhairi Othman ◽  
Anita Tilwari ◽  
...  
Keyword(s):  
Cell Death ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Autophagic Cell Death ◽  
Biologically Active ◽  
Vero Cell Lines ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

Eupatorium odoratum (EO)contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action ofEOextracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity, membrane blebbing and ballooning at 24 hours, replacement by mass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts ofEOinduce cell death through induction of autophagy and hold potential for development as potential anticancer drugs.

scholarly journals In vitro anticancer property of Solanum mammosum callus culture against HeLa and Vero cell lines

2020 ◽  
Vol 24 (2) ◽  
pp. 218-224
Author(s):  
SUCIATI SUCIATI ◽  
Lusiana ARIFIANTI ◽  
Andiena ELSAFIRA ◽  
Lovely Q. ILMIAH
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Callus Culture ◽  
Solanum Mammosum ◽  
Vero Cell Lines

scholarly journals CYTOTOXIC ACTIVITIES IN VITRO OF FLOWER EXTRACTS OF THREE SPECIES OF ALOE GROWING IN YEMEN:ALOE RUBROVIOLACEAE, ALOE VERA AND ALOE SABAEA, AGAINST ELEVEN TYPES OF CANCER CELL LINES

2021 ◽  
Author(s):  
Hassan Mohammed Al-Mahbashi ◽  
Mohammad Abobakr Al-Ghazali ◽  
Hassan A. Al-Shamahy ◽  
Azhar Azher Mohammed Al-Ankoshy
Keyword(s):  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Peer Review ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Aloe Vera ◽  
Cancer Cell Lines ◽  
Ethanolic Extracts

 Background and aims: Natural products, in particular plant extracts, have opened up great chance in the area of drug progress owing to their chemical variety. The Aloe genus has long been known to be used for medicinal uses in countless parts of the world. This study was planned to inspect the phytochemicals and anti-cancer capabilities of Aloe rubroviolaceae, Aloe vera and Aloe sabaea flowers. Materials and Methods: Three types of ethanolic extracts of plants traditionally used in Yemen to treat a variety of diseases have been tested in vitro for their potential anticancer activity on different human cancer cell lines. The cytotoxic activity of the ethanolic extracts of tested plants was determined using eleven strains of human cancer cells, namely: MCF-7 (breast cancer), PC-3 (prostate cancer), HEP-2(human epithelial carcinoma), MNFS-60 (myelogenous leukemia), CACO (intestinal cancer), A-549 (lung adenocarcinoma), HeLa (cervical cancer),RD (rhabdomyosarcoma), HepG2 (hepatocellular carcinoma), HCT-116 (colon cancer),  and CHO-K1(Chinese hamster ovary). A colorimetric sulforhodamine B assay was applied to assess the in vitro cytotoxic activity of various extracts. Growth inhibition of 50% (IC50) for each extract was calculated from the optical density of treated and untreated cells. Doxorubicin, a broad-spectrum anticancer drug was used as a positive control. Results: More interesting cytotoxic activity was observed for Aloe vera extract more than Aloe sabaea and Aloe rubroviolaceae, extract.  Conclusions: This study presents an initial screening for anti-proliferative activity of a variety of Aloe species flowers extracts on diverse cancer cell lines. Different extracts of Aloe species significantly inhibit the growth of various cancer cell lines  in a concentration-dependent manner. Advance researches are necessary to understand the possible mechanism(s) of action of these extract on a variety of cancer cells and separation of active phyto-chemicals.                   Peer Review History: Received: 18 July 2021; Revised: 17 August; Accepted: 8 September, Available online: 15 September 2021 Academic Editor:  Dr. Muhammad Zahid Iqbal, AIMST University, Malaysia, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.5/10 Average Peer review marks at publication stage: 7.5/10 Reviewers: Dr. U. S. Mahadeva Rao, Universiti Sultan Zainal Abidin, Terengganu Malaysia, [email protected] Dr. Nazim Hussain, BFIT, Dehradun, Uttarakhand, India, [email protected] Similar Articles: ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS IN VITRO INHIBITORY ACTIVITY OF BERBERIS VULGARIS L. AGAINST LEISHMANIA TROPICA PROMASTIGOTES

Synthesis, In Silico and In Vivo Evaluation of Novel 1, 3, 4-Thiadiazole Analogues as Novel Anticancer Agents

2020 ◽  
Vol 17 (4) ◽  
pp. 434-444 ◽  
Author(s):  
Swathi Krishna ◽  
Byran Gowramma ◽  
Manal Mohammed ◽  
Rajagopal Kalirajan ◽  
Lakshman Kaviarasan ◽  
...  
Keyword(s):  
Vero Cell ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Binding Interaction ◽  
Standard Drug ◽  
Discovery Studio ◽  
Vero Cell Lines ◽  
Mcf 7

Background: 1,3,4-thiadiazole is a lead molécule which is versatile for a wide variety of biological activities and in continuation of our interest in establishing some novel heterocyclic compounds for antitumor activity. Objective: The objective of the study was to synthesize series of 5-(1,3-benzodioxol-5-yl)-1,3,4- thiadiazol-2-amine derivative and evaluated for their possible in vitro and in vivo anticancer activity. Methods: The synthesis of 2-aminonaphthoxy-1,3,4-thiadiazole and 5-(1,3-benzodioxol-5-yl)-1,3,4- thiadiazol-2-amine as intermediates were carried out by cyclization method. A mixture of thiosemicarbazide and naphthoxyacetic acid/piperonylic acid and phosphoryl chloride were subjected to cyclization with phosphorous oxychloride to obtain compound 3. Further compounds 1 and 3 were reacted with different aromatic aldehydes in methanol to form compounds 2a-e and 4a-e. The compounds 2a-e and 4a-e were characterized for the melting points, IR, Proton NMR and Mass spectra. The compounds were further evaluated for their anticancer activity. The docking study was performed using Discovery studio 4.1 (Accelrys) software against DNA-binding domain of STAT3. The compounds were analyzed for the ligand-protein binding interaction(s) by molecular docking into the active site of STAT3β using the CDOCKER protocol of Discovery studio (v4.1). Results: The title compounds were screened for in vitro anticancer on human breastadenocarcinoma cells (MCF-7 and Vero). Compounds 4c, 4d and 2d against MCF 7 and 4d against Vero cell lines were found to be the most active dérivatives with IC50 values of 1.03, 2.81 and 3.45 µg/ml against MCF 7 and 31.81 µg/ml against Vero cell lines, respectively. Conclusion: From the in vivo anticancer studies, it was concluded that the synthesized compounds 4c and 4d displayed anticancer activity comparable to the standard drug, while the rest of the compounds demonstrated mild potency for anticancer studies.

scholarly journals In Vitro Anticancer and Cytotoxic Activities of Some Plant Extracts on HeLa and Vero Cell Lines

Proceedings ◽  
2017 ◽  
Vol 1 (10) ◽  
pp. 1019 ◽  
Author(s):  
Fulya Tugba Artun ◽  
Ali Karagoz ◽  
Gul Ozcan ◽  
Gulay Melikoglu ◽  
Sezin Anil ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Plant Extracts ◽  
Cytotoxic Activities ◽  
Vero Cell Lines

scholarly journals Chemical Composition and Cytotoxic and Antibacterial Activities of the Essential Oil of Aloysia citriodora Palau Grown in Morocco

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Moulay Ali Oukerrou ◽  
Mounir Tilaoui ◽  
Hassan Ait Mouse ◽  
Inass Leouifoudi ◽  
Abdeslam Jaafari ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Cytotoxic Effect ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Agar Disc ◽  
Vero Cell Lines

The aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of Aloysia citriodora Palau, harvested in different regions of Morocco. The chemical profile was established using gas chromatography-mass spectrometry analysis. The cytotoxic activity against P815, MCF7, and VERO cell lines as well as the normal human peripheral blood mononuclear cells (PBMCs) was evaluated using the MTT assay. Standard, ATCC, strains of bacteria (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) were cultivated in Muller Hinton media. Then, agar disc diffusion, minimum inhibitory concentrations (MICs), and minimal bactericidal concentrations (MBCs) were determined using microdilution method. The essential oils obtained were predominantly composed of β-spathulenol (15.61%), Ar-curcumene (14.15%), trans-caryophyllene oxide (14.14%), and neral (10.02%). The results of the assays showed that the cytotoxic effect of the essential oil of A. citriodora was high on P815 and moderate on MCF7 and on VERO cell lines. However, no cytotoxic effect was observed on PBMCs. On the other hand, essential oils showed a significant antimicrobial activity against both Gram-negative and Gram-positive bacteria. MICs ranged between 2.84 and 8.37 mg/ml. Essential oil of A. citriodora leaves possesses significant antibacterial effect and cytotoxic activity against tumor cell lines.

scholarly journals Pengujian Toksisitas Seluler SNEDDS Ekstrak Daun Kangkung (Ipomoea reptans, Poir) Terstandar

2019 ◽  
Vol 6 (2) ◽  
pp. 48
Author(s):  
Lutfi Chabib ◽  
Farida Hayati ◽  
Rizki Awaluddin ◽  
Muh Iqbal Pangestu
Keyword(s):  
Cell Death ◽  
Vero Cell ◽  
Cell Lines ◽  
Control Cell ◽  
Test Sample ◽  
Methyl Thiazolyl Tetrazolium ◽  
Control Medium ◽  
Vero Cell Lines ◽  
And Control

ABSTRAK Kangkung darat (Ipomea reptans Poir) adalah salah satu tanaman di Indonesia yang secara empiris telah dimanfaatkan oleh masyarakat sebagai obat untuk terapi Diabetes Melitus (DM).  Riset sebelumnya telah dilakukan untuk memperoleh data ilmiah dari pemanfaatan kangkung darat sebagai terapi DM, dan pengembangannya dalam bentuk sediaan Self Nano Emulsifying Drug Delivery System (SNEDDS). Penelitian ini bertujuan untuk mengetahui sitotoksisitas sediaan SNEDDS ekstrak daun kangkung terstandar dengan menggunakan metode MTT (Methyl-Thiazolyl-Tetrazolium). Pengujian MTT dilakukan dengan cara well plate diplotkan terlebih dahulu untuk sampel uji 70 sumuran (well), kontrol sel 6 sumuran, dan kontrol media 6 sumuran.  Terakhir tiap sumuran ditambahkan stopper SDS sebanyak 100µl. Plate dibungkus alumunium foil dan diinkubasi semalaman pada suhu ruang. Hari berikutnya dilakukan pembacaan absorbansi sampel, kontrol media, dan kontrol sel menggunakan ELISA reader. Uji sitotoksik SNEEDS kangkung pada sel vero diperoleh hasil bahwa SNEEDS kangkung tidak menyebabkan kematian pada sel uji. Pada pengujian sel dilakukan pemaparan sampel excipient dari SNEEDS kangkung sebagai pembanding, dengan hasil menyebabkan kematian <50% sel uji pada dua kadar tertinggi dari sampel excipient. Namun pada hasil uji SNEEDS kangkung diperoleh data bahwa toksisitas excipient pada kadar tersebut tidak mempengaruhi timbulnya toksisitas pada sampel SNEEDS kangkung. Sehingga berdasarkan uji in vitro, SNEEDS kangkung tidak toksik terhadap sel vero. Kata kunci: Ipomoea reptans, sitotoksisitas, SNEDDS  ABSTRACT Kangkong (Ipomea reptans Poir) is a type of Indonesian plant empirically utilized by many people to treat diabetes mellitus (DM). A study has been conducted to obtain scientific data from the use of kangkong for DM treatment as well as to develop kangkong in SNEDDS preparation. This current study aimed to examine the cytotoxicity of kangkong leaf standardized extract in SNEDDS preparation through the MTT (Methyl-Thiazolyl-Tetrazolium) method. MTT assay was performed by initially plotting well plates for test sample (70 wells), control cell (6 wells), and control medium (6 wells). As much as 100µl SDS stopper was added into each well, and then plates were wrapped in aluminum foil and incubated all night at ambient temperature. On the following day, the absorbance of test sample, control medium, and control cell was identified using ELISA reader. The cytotoxicity test of kangkong SNEDDS on Vero cell lines showed that kangkong SNEDDS did not cause cell death. The cell was tested through exposure of excipient sample from kangkong SNEDDS as a comparison, resulting in <50% cell death by the two highest concentrations of excipient sample. However, the test result of kangkong SNEDDS indicated that excipient toxicity at such concentrations did not affect kangkong SNEDDS. Therefore, based on in vitro test, kangkong SNEDDS is not toxic against Vero cell lines. Keywords: Ipomoea reptans, cytotoxicity, SNEDDS

scholarly journals CYTOTOXIC ACTIVITIES PROFILE OF PARASITE MANGO (Dendrophthoe pentandra) FROM VARIOUS AREAS IN INDONESIA AGAINST T47D BREAST CANCER CELLS AND NORMAL VERO CELL LINES

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Roihatul Mutiah
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cancer Cells ◽  
Cell Lines ◽  
Ethanol Extract ◽  
Assay Method ◽  
Cytotoxic Activities ◽  
Activity Profile ◽  
Three Samples ◽  
Vero Cell Lines

<p><em>Indonesia has so many kinds of a parasite (mistletoe), one of them which has potential anticancer activity is mango parasite (Dendrophthoe pentandra). This research aimed at determining the cytotoxic activity profile of mango parasite leaves extracts from several locations in Indonesia against T47D and Vero cell lines. Mango parasite leaves obtained from 4 locations in Indonesia: Kediri, Pekalongan, Sumatra, and Kalimantan. Three samples was obtained from 3 different sub-districts in each locations. Cytotoxic activities of samples were performed by MTT assay method. The results of this study showed that there were differences between samples obtained from different locations, especially in cytotoxic activity and selectivity indexes of mango parasite leaves (D. pentandra) ethanol extract. D. pentandra obtained from Kalimantan has the most potential cytotoxic activity and could be potentially developed into a T47D breast anticancer agent. Extracts from the Kalimantan have an IC<sub>50</sub> values of287.39 μg/ml, LC<sub>50 </sub>798.28 μg / ml and selectivity index 2.77, which means it was toxic to cancer cells but safe for Vero cell lines, and its selectively kills cancer cells rather than Vero cell lines. The result of this study could support D. pentandra to be developed as a new potential Fitofarmaka.</em></p>

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