scholarly journals Sensitive and Rapid HPLC Method for Determination of Memantine in Human Plasma Using OPA Derivatization and Fluorescence Detection: Application to Pharmacokinetic Studies

2010 ◽  
Vol 78 (4) ◽  
pp. 847-856 ◽  
Author(s):  
Afshin Zarghi
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
Hplc Method ◽  
Pharmacokinetic Studies

A Sensitive HPLC Method for the Determination of Fluoxetine and Norfluoxetine in Human Plasma with Fluorescence Detection

2005 ◽  
Vol 27 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Sdka Ert??rk ◽  
Sevil M??ge ??etin ◽  
Sedef Atmaca ◽  
Lale Ersoy ◽  
G??l Baktr
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
Hplc Method

scholarly journals Bioanalytical Method Development and Validation for the Determination of Favipiravir in Spiked Human Plasma by using RP-HPLC

2021 ◽  
pp. 275-281
Author(s):  
Pallavi V. Duse ◽  
Kamalkishor G. Baheti
Keyword(s):  
Human Plasma ◽  
Hplc Method ◽  
Therapeutic Drug ◽  
Pharmacokinetic Studies ◽  
Gradient System ◽  
Spiked Human Plasma ◽  
Rp Hplc ◽  
Relative Standard ◽  
Limit Of Quantitation

A precise, simple and reproducible reverse phase liquid chromatography (RP-HPLC) method was developed and validated for determination of Favipiravir by using Carbamazepine as internal standard in spiked human plasma. A chromatographic separation was accomplished with Cromasil C18 (250mm x 4.6ID, Particle size: 5 micron) column using mobile phase consists of methanol: water in the ratio (35:65, %v/v), at pH 3.0 with binary gradient system-maintained flow rate at 0.8ml/min. The detection wavelength of drug sample was at 225 nm. Extraction was done by using ethyl acetate as extracting solvent. The retention time of Favipiravir was found to be 6.62 min.  The method was found to be linear in the concentration range of 0.2-3.2 µg/ml. Limit of quantitation (LOQ) value was found to be 0.72. The intra- and inter day precision and accuracy lies within the specified range. The recovery studies were found to be in the range of 97.6 to 100.2%. %Relative standard deviation (RSD) was found to be in the range of 0.07-2.80%. All parameters were found to be validated from spiked human plasma. The proposed RP-HPLC method is highly accurate and rapid for the determination of favipiravir in human plasma and can be applied for pharmacokinetic studies and Therapeutic drug monitoring.

scholarly journals Nonextractive Procedure and Precolumn Derivatization with 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole for Trace Determination of Trimetazidine in Plasma by High-Performance Liquid Chromatography with Fluorescence Detection

2008 ◽  
Vol 91 (5) ◽  
pp. 1037-1044 ◽  
Author(s):  
Ibrahim A Darwish ◽  
Ashraf M Mahmoud ◽  
Nasr Y Khalil
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Pharmacokinetic Studies ◽  
Precolumn Derivatization ◽  
Trace Determination ◽  
Relative Standard

Abstract A highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed and validated in a single laboratory for the trace determination of trimetazidine (TMZ) in human plasma. Fluoxetine (FLX) was used as the internal standard. TMZ and FLX were isolated from plasma by protein precipitation with acetonitrile and derivatized by heating with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole in pH 8 borate buffer at 70C for 30 min. Separations were performed in the isocratic mode on a Nucleosil CN column with the mobile phase acetonitrile10 mM sodium acetate buffer (pH 3.5)methanol (47 + 47 + 6, v/v/v) at a flow rate of 1.0 mL/min. The derivatized samples were excited at 470 nm and monitored at an emission wavelength of 530 nm. Under the optimum chromatographic conditions, a linear relationship with a good correlation coefficient (r 0.9997, n 5) was obtained for the peak area ratio of TMZ to FLX and for TMZ concentrations of 1120 ng/mL. The proposed method has the lowest limits of detection and quantitation reported to date for the determination of TMZ in plasma with values of 0.3 and 0.95 ng/mL, respectively. The values for intra- and interassay precision were satisfactory; the relative standard deviations were 4.04. The accuracy of the method was demonstrated; the recoveries of TMZ from spiked human plasma were 98.13102.83 0.24.04. The method has high throughput because of its simple sample preparation procedure and short run time (<10 min). The results demonstrated that the proposed method would have great value when applied in pharmacokinetic studies for TMZ.

Simple and Sensitive HPLC Method for Determination of Gemfibrozil in Human Plasma with Fluorescence Detection

2006 ◽  
Vol 29 (3) ◽  
pp. 403-414 ◽  
Author(s):  
Chong‐Kook Kim ◽  
Jun‐Pil Jae ◽  
Hye‐Ryung Hwang ◽  
Eunmi Ban ◽  
Jeong‐Eun Maeng ◽  
...  
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
Hplc Method
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