scholarly journals Effect of Time-Lapse Administration of Panadol (Paracetamol) on Spleen and Kidney Functions of Adult Albino Mice

2018 ◽  
Vol 9 (2) ◽  
pp. 88-93
Author(s):  
Muddasir Hassan Abbasi ◽  
Komal David ◽  
Muhammad Idnan ◽  
Zaira Ahmed ◽  
Asif Mahmood Qureshi
Keyword(s):  
Adverse Effects ◽  
Age Groups ◽  
Time Lapse ◽  
Control Group ◽  
Time Interval ◽  
White Pulp ◽  
Tissue Samples ◽  
Kidney Section ◽  
Kidney Functions ◽  
Histopathological Effects

Panadol is a remarkable pain and fever reducing non opioid drug. It is known to be completely safe and tolerant medicine throughout the globe among people of all age groups. The goal of this study was to elaborate the histopathological effects of reduced interrupted regime of panadol on spleen and kidney functions of mice (Mus musculus). The animals were categorized into four groups, the control group (C), and rest of the three were labeled as 1, 2 and 3, made on the basis of time interval of pandol administration via gavage (n=7). Matched volume of panadol (15000 µg/0.1ml) and normal saline was given to mice.  Tissue samples were collected after sacrifice of the mice and processed for assessment. The findings of the current study reflected the histopathological damage of kidney and spleen caused by panadol in reduced interval of time. The kidney section illustrated clear distortion in glomeruli integrity, marked increase in interstitial spaces, damaged epithelia, and degeneration in tubules in all the groups. The spleen histology exhibited the degradation of white pulp, depopulation, activation of follicles, cellular disruption thereby overall disorganized stature. The raised values of serum creatinine and blood urea examinations also revealed the deleterious effects of panadol overconsumption. It is inferred from the above mentioned outcomes that though panadol is considered to be a safe drug even then its intake prior to four hours can account for adverse effects on kidney and spleen.

scholarly journals The Effect of Zingiber officinale on the Spleen Tissue and Antibody Titer of Broiler Chickens

2019 ◽  
Vol 36 (01) ◽  
pp. 046-050
Author(s):  
Alireza Taghdisi ◽  
Sajjad Hejazi
Keyword(s):  
Broiler Chickens ◽  
Zingiber Officinale ◽  
Control Group ◽  
White Pulp ◽  
Broiler Chicks ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Serum Factors ◽  
Spleen Tissue ◽  
Significant Difference

Introduction Increasing the immune system's function of fighting infectious diseases is very important in the poultry industry. Ginger, scientifically known as Zingiber officinale, belongs to the Zingiberaceae family. The use of ginger in the diet of poultry increases serum levels of superoxide dismutase enzymes and glutathione peroxidase, which are considered to be important antioxidant enzymes. The main objective of the present study is to evaluate the effect of ginger on the spleen tissue of broiler chickens. Material and Methods The specimens comprised 2 groups of 20 Ross breed broiler chicks, for 42 days and were then, examined and tested. The diet was supplemented with 1 g/kg of ginger powder from the beginning of the rearing period. Blood samples of the chicks were randomly collected to measure the levels of hemagglutination (HI). The removed spleens were fixed with 10% formalin buffer. The specimens were cut in 5-micron diameters and stained with hematoxylin and eosin. Results and Conclusion There was a statistically significant difference in the mean of HI blood titers between the chicks in the growth period and final period groups (p < 0.05). The white-pulp tissue samples were more clearly seen in the treatment group than in the control group, and also, it was observed that the wall of the central artery of the white pulp was thicker in the ginger-treated group as compared with the control group. The nutritional value of ginger may vary. Thus, it is necessary to investigate the effect of this plant final on weight gain; the serum factors associated with the metabolic chart, and the response of the immune system to this plant.

scholarly journals Effects of Aflatoxin on Liver and Protective Effectiveness of Esterified Glucomannan in Merino Rams

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Fatma Colakoglu ◽  
Hasan Hüseyin Donmez
Keyword(s):  
Adverse Effects ◽  
Control Group ◽  
Feeding Period ◽  
Total Aflatoxin ◽  
Vacuolar Degeneration ◽  
Tissue Samples ◽  
Hydropic Degeneration ◽  
Commercial Feed ◽  
One Year ◽  
Protective Effectiveness

The effects of total aflatoxin (AF) given orally on liver in Merino rams were studied. In addition, this study was conducted in order to evaluate the efficacy of an esterified glucomannan (EG) for protection against aflatoxicosis. One-year-old 32 Merino rams were divided into four equal groups. The control group (C) was fed with the commercial feed. The AF group was fed with commercial feed plus 250 μg/day of total AF. The EG group was fed with commercial feed plus 2 g/day of EG. The AF + EG group was fed with commercial feed plus 250 μg/day of total AF and 2 g/day of EG. After feeding period, tissue samples were taken from the liver in order to perform histological analyses. Vacuolar degeneration with small and large droplets and hydropic degeneration in hepatocytes were observed in the AF group. The ceroid pigmentation was observed in macrophages in groups or one by one. It was observed that the fat rate in hepatocytes was 2.6% in the C group, 35.5% in the AF group, 2.9% in the EG group, and 9.6% in the AF + EG group. In conclusion, the adverse effects caused by aflatoxicosis on the liver could be ameliorated by adding EG to the ration.

Alterations of Small Intestinal Morphology on Villi and Crypts after Feeding Probiotic and Zinc in Pre and Post-weaned Piglets

2020 ◽  
Author(s):  
Arup Kalita ◽  
M. Talukdar ◽  
K. Sarma ◽  
P.C. Kalita ◽  
C. Gautam ◽  
...  
Keyword(s):  
Treatment Group ◽  
Age Groups ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Economic Losses ◽  
Control Group ◽  
Gut Health ◽  
Large White ◽  
Tissue Samples ◽  
Age Related

Background: Neonatal and early post-weaned piglet mortality causes huge economic losses to the farmers. The beneficial effect of probiotic and zinc in respect to the gut health in piglets has already been established. Therefore, this study was aimed to evaluate age-related changes and the effect of dietary inclusion of probiotic and zinc on morphological and morphometrical characteristics on villi and crypts of the small intestine in pre and post-weaned piglets.Methods: A total of 18 Large White Yorkshire piglets, irrespective of sex were divided into three different age-groups [pre-weaning (20 days old, n = 3) and, post-weaning (30 days old, n = 3) and (60 days old, n = 3)]. The piglets were weaned at 28 days of age. They were divided into control group (C) fed with basal diet and treatment group (T) fed with combined probiotic and zinc oral supplement along with the basal diet. After sacrifice the animals, tissue samples were collected and processed for routine stain and SEM. The alterations on morphology and morphometry of villi and crypts were recorded both in the control and treatment group of piglets. Result: The villus height and width and crypt depth and width were increased, however, the villus crypt ratio was found to be lower in the treatment group of piglets. The villus and crypt enlargement factor showing higher numerical values in the piglets fed with probiotic and zinc than the control group of piglets. Scanning electron microscopy revealed a clear extrusion zone with prominent shedding of cells in the villi of jejunum and ileum in the treatment group of piglets at day 30 and 60.

scholarly journals PHYSIOLOGICAL STUDY OF OLIVE OIL EFFECT ON IBUPROFEN-INDUCED LIVER AND KIDNEY DYSFUNCTION IN LOCAL MALE RABBITS

2020 ◽  
Vol 1 (4) ◽  
Author(s):  
Abdulrahman Salem Omer Yaseen ◽  
Khaled Saleh Omer Abdulmanea ◽  
Rafat Mohsen Hussein Salem
Keyword(s):  
Adverse Effects ◽  
Olive Oil ◽  
Group Treatment ◽  
Control Group ◽  
Beneficial Effects ◽  
Ameliorative Effect ◽  
Liver And Kidney ◽  
Inflammatory Drugs ◽  
Functional Capacities ◽  
Kidney Functions

Ibuprofen (IB), is one of the common nonsteroidal anti-inflammatory drugs (NSAIDs), it is mostly used for the management of pain, fever, and inflammations. In addition to its beneficial effects; IB has also been reported to be associated with some adverse effects. Thus, the present study was aimed to investigate the possible beneficial effects of olive oil (OO) against the IB -induced dysfunction of liver and kidney parameters in male rabbits. This study was conducted on 18 local male rabbits weighing between (900 - 1000 g) for 30 days, rabbits were divided into three equal groups; control group (C), ibuprofen group (IB), and ibuprofen +olive oil group (IB + OO), each group included six rats. At the end of the experiment, blood samples were collected for biochemical assessment of the liver and kidney functions. The results showed that IB caused a significant increase (P˂ 0.001) in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea (Ur), creatinine (Cr), and uric acid (UA). A significant decrease in the total protein (TP) and albumin (AL) (P˂ 0.001) and (P˂ 0.05) respectively showed in the IB group compared to the control group. Treatment rabbits with OO showed significant improvement in renal and liver functions; the activity enzymes of ALT, AST, and ALP significantly decreased, and the levels of TP and AL significantly increased. The current results were in agreement with other previous studies which informed that the IB has adverse effects on the functional capacities of the liver and the kidney. Further studies are needed to evaluate this ameliorative effect of OO on other medicines and pollutants.

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

Employing Gamma Ray Irradiated Giardia lamblia as Trialed Vaccine in Experimental Animal

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Amal Kamil Abdul Sada ◽  
Amany Mohamed Al-Kaysi
Keyword(s):  
Giardia Lamblia ◽  
Gamma Ray ◽  
Age Groups ◽  
Active Phase ◽  
Study Groups ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Histopathological Changes ◽  
Gamma Irradiated

This is an experimental trial to prepare a vaccine from gamma-irradiated Giardia lamblia which is evaluated in experimental animals. The study was conducted from December 2015 to April 2016. The field survey of the parasite was conducted from those patients attending the laboratories of the Alawi Children's Hospital in Rusafa and the Al-Yarmouk Teaching Hospital in Karkh, through which 1250 stool samples of different age groups were examined. Five groups of mice were used in the study; the first was injected with normal saline and considered as a negative control group, the second was injected with cystic form of non-irradiated Giardia lamblia and considered as a positive control group, whereas the other three groups were injected with gamma irradiated Giardia lamblia at three different doses 10, 15 and 25 rad respectively. Giardia lamblia was primarily cultivated in liver infusion agar for ten days to obtain the active phase. On the sixth day, the cystic phase was purified and standardized to be used in the infection of mice with or without the exposure of gamma rays. Mice showed high sensitivity to parasitic infestation, in the gamma non-irradiated and the irradiated with gamma 10 rad, and 15 rad irradiated groups which was 100%. The results expressed an excystation process of the depleted phases and the release of the feeder phases. The results of the three irradiated groups consisted of histopathological changes of the small, and the rectum by dissection after two weeks of infection, with intestine amputation lesions, as well as ulceration and inflammation of the inflammatory cells represented in small numbers of neutrophil, lymphocytes, and eosinophils. The presence of ulceration and fall of epithelial cells in the intestinal cavity has been shown, and different forms of the parasite have been observed. Mice which was injected with irradiated G lamblia at high dose (25 rad), not show and sensitivity to the challenge infection and no excystation of thy parasite had been done. After 2 wreaks, a comparison was achieved between all study groups in which no histopathological changes were noticed in the mice irradiated with dose of25 rad. After another two weeks, a challenge dose was given (un-attenuated G lamblia) and mice were dissected after another two weeks, no changes on the level of histopathology of intestinal tissue were noticed the results suggested that mice acquire an immunity against the parasite infection.

CLINICAL SIGNIFICANCE OF SERUM CAVEOLIN-1 LEVELS IN GASTRIC CANCER PATIENTS

2018 ◽  
Vol 40 (4) ◽  
pp. 323-327 ◽  
Author(s):  
F Tas ◽  
S Karabulut ◽  
K Erturk ◽  
D Duranyildiz
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Histological Grade ◽  
Clinical Importance ◽  
Disease Stage ◽  
Control Group ◽  
Tissue Samples ◽  
Caveolin 1 ◽  
Study Results ◽  
Gastric Cancer Patients

Aim: Caveolin-1 plays a significant role in the pathogenesis of various carcinomas and its expression affects the survival of cancer patients. However, the molecular function of caveolin-1 and its possible clinical importance has remained uncertain in gastric cancer. No clinical trial has examined serum caveolin-1 levels in gastric cancer patients so far, instead all available results were provided from studies conducted on tissue samples. In the current study, we analyzed the soluble serum caveolin-1 levels in gastric cancer patients, and specified its associations with the clinical factors and prognosis. Material and Methods: Sixty-three patients with pathologically confirmed gastric cancer were enrolled into the trial. Serum caveolin-1 concentrations were detected by ELISA method. Thirty healthy subjects were also included in the study. Results: The median age of patients was 62 years, ranging from 28 to 82 years. The serum caveolin-1 levels in gastric cancer patients were significantly higher than those in control group (p < 0.001). The common clinical parameters including patient age, sex, lesion localization, histopathology, histological grade, disease stage, and various serum tumor markers (e.g. LDH, CEA, and CA 19.9) were not found to be associated with serum caveolin-1 levels (p > 0.05). Similarly, no correlation existed between serum caveolin-1 concentration and chemotherapy responsiveness (p = 0.93). Furthermore, serum caveolin-1 level was not found to have a prognostic role (p = 0.16). Conclusion: Even though it is neither predictive nor prognostic, serum caveolin-1 level may be a valuable diagnostic indicator in patients with gastric cancer. Key

scholarly journals Arginase Isoform Expression in Chronic Rhinosinusitis

2019 ◽  
Vol 8 (11) ◽  
pp. 1809 ◽  
Author(s):  
Diana Vlad ◽  
Silviu Albu
Keyword(s):  
Nitric Oxide ◽  
Chronic Rhinosinusitis ◽  
Defense Mechanisms ◽  
Upper Airway ◽  
Control Group ◽  
Mrna Levels ◽  
Tissue Samples ◽  
Endoscopic View ◽  
Important Regulator ◽  
Arginase I

Nitric oxide (NO) has emerged as an important regulator of upper airway inflammation, mainly as part of the local naso-sinusal defense mechanisms. Increased arginase activity can reduce NO levels by decreasing the availability of its precursor, L-arginine. Chronic rhinosinusitis (CRS) has been associated with low levels of nasal nitric oxide (nNO). Thus, the present study investigates the activity of arginase I (ARG1) and II (ARG2) in CRS and its possible involvement in the pathogenesis of this disease. Under endoscopic view, tissue samples of pathologic (n = 36) and normal (n = 29) rhinosinusal mucosa were collected. Arginase I and II mRNA levels were measured using real-time PCR. Our results showed low arginase I activity in all samples. The levels of ARG2 were significantly higher in patients with chronic rhinosinusitis compared to the control group (fold regulation (FR) 2.22 ± 0.42 vs. 1.31 ± 0.21, p = 0.016). Increased ARG2 expression was found in patients with CRS without nasal polyposis (FR 3.14 ± 1.16 vs. 1.31 ± 0.21, p = 0.0175), in non-allergic CRS (FR 2.55 ± 0.52 vs. 1.31 ± 0.21, p = 0.005), and non-asthmatic CRS (FR 2.42 ± 0.57 vs. 1.31 ± 0.21, p = 0.028). These findings suggest that the upregulation of ARG2 may play a role in the pathology of a distinctive phenotype of CRS.

scholarly journals Cyanobacteria, cyanotoxins, and their histopathological effects on fish tissues in Fehérvárcsurgó reservoir, Hungary

2021 ◽  
Vol 193 (9) ◽  
Author(s):  
Damjana Drobac Backović ◽  
Nada Tokodi ◽  
Zoran Marinović ◽  
Jelena Lujić ◽  
Tamara Dulić ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Fish Tissue ◽  
Freshwater Ecosystems ◽  
Abramis Brama ◽  
Histopathological Changes ◽  
Fish Tissues ◽  
The World ◽  
Encoding Gene ◽  
Microcystis Wesenbergii ◽  
Histopathological Effects

AbstractCyanobacteria are important members of lake plankton, but they have the ability to form blooms and produce cyanotoxins and thus cause a number of adverse effects. Freshwater ecosystems around the world have been investigated for the distribution of cyanobacteria and their toxins and the effects they have on the ecosystems. Similar research was performed on the Fehérvárcsurgó reservoir in Hungary during 2018. Cyanobacteria were present and blooming, and the highest abundance was recorded in July (2,822,000 cells/mL). The species present were Aphanizomenon flos-aquae, Microcystis flos-aquae, Microcystis wesenbergii, Cuspidothrix issatschenkoi, Dolichospermum flos-aquae, and Snowella litoralis. In July and September, the microcystin encoding gene mcyE and the saxitoxin encoding gene sxtG were amplified in the biomass samples. While a low concentration of microcystin-RR was found in one water sample from July, analyses of Abramis brama and Carassius gibelio caught from the reservoir did not show the presence of the investigated microcystins in the fish tissue. However, several histopathological changes, predominantly in gills and kidneys, were observed in the fish, and the damage was more severe during May and especially July, which coincides with the increase in cyanobacterial biomass during the summer months. Cyanobacteria may thus have adverse effects in this ecosystem.

scholarly journals PETTLEP imagery and tennis service performance: an applied investigation

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nafih Cherappurath ◽  
Masilamani Elayaraja ◽  
Dilshith A. Kabeer ◽  
Amila Anjum ◽  
Paris Vogazianos ◽  
...  
Keyword(s):  
Age Groups ◽  
Training Session ◽  
Performance Outcomes ◽  
Service Performance ◽  
Control Group ◽  
Specific Training ◽  
Tennis Players ◽  
Imagery Training ◽  
Before And After ◽  
Junior Tennis

AbstractTennis is one of the most popular and widely played sports enjoyed by players of different age groups and genders as a profession as well as a mode of recreation. A novel method, PETTLEP imagery combines both conventional and non-conventional style of training of an athlete and improves one’s performance. This study aimed to analyze the tennis service performance of junior tennis players based on PETTLEP imagery training. Forty-four junior male tennis players (Mage=13.22 years, SD=0.42) were selected for the study. The investigator handed over the MIQ-R questionnaire to all the participants in which they scored 16 and above points as per previous research. The participants were equally divided (n=11) into three experimental groups (E1, E2, and E3) and a control group. The service performance outcomes of all the players were compared before and after a training session. The three experimental groups were assigned with service-specific training, service-specific training combined with PETTLEP imagery training, and PETTLEP imagery training alone, respectively, for three days per week for 12 weeks. They were tested on their service accuracy based on the International tennis number (ITN) manual on-court assessment test. The data were assessed for normality and analyzed using non-parametric methods to reveal main effects (each training method alone) as well as to calculate the combined effect of PETTLEP and service-specific training. Certain significant improvements in tennis service were observed with service-specific training alone. Though it marginally outperformed the PETTLEP imagery method, the most improved services were observed with both PETTLEP and service-specific training utilized together. This implies an additive effect when both methods are used together.

Sign in / Sign up

Export Citation Format

Share Document