scholarly journals Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

10.3791/990 ◽  
2009 ◽  
Author(s):  
Hae Young Lee ◽  
Lloyd A. Greene ◽  
Carol A. Mason ◽  
M. Chiara Manzini
Keyword(s):  
Progenitor Cells ◽  
Cerebellar Granule Neuron ◽  
Cerebellar Granule ◽  
Granule Neuron

scholarly journals Reciprocal actions of ATF5 and Shh in proliferation of cerebellar granule neuron progenitor cells

2012 ◽  
Vol 72 (6) ◽  
pp. 789-804 ◽  
Author(s):  
Hae Young Lee ◽  
James M. Angelastro ◽  
Anna Marie Kenney ◽  
Carol A. Mason ◽  
Lloyd A. Greene
Keyword(s):  
Progenitor Cells ◽  
Cerebellar Granule Neuron ◽  
Cerebellar Granule ◽  
Granule Neuron

scholarly journals Shh-Mediated Increase in β-Catenin Levels Maintains Cerebellar Granule Neuron Progenitors in Proliferation

2020 ◽  
Vol 19 (5) ◽  
pp. 645-664 ◽  
Author(s):  
Shyamala Mani ◽  
Saranya Radhakrishnan ◽  
Rajit Narayanan Cheramangalam ◽  
Shalini Harkar ◽  
Samyutha Rajendran ◽  
...  
Keyword(s):  
Cerebellar Granule Neuron ◽  
Cerebellar Granule ◽  
Granule Neuron

scholarly journals Forkhead Transcription Factor FoxM1 Regulates Mitotic Entry and Prevents Spindle Defects in Cerebellar Granule Neuron Precursors

2007 ◽  
Vol 27 (23) ◽  
pp. 8259-8270 ◽  
Author(s):  
Ulrich Schüller ◽  
Qing Zhao ◽  
Susana A. Godinho ◽  
Vivi M. Heine ◽  
René H. Medema ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Target Genes ◽  
Cyclin B1 ◽  
Null Alleles ◽  
Cerebellar Granule Neuron ◽  
Forkhead Transcription Factor ◽  
Cerebellar Granule ◽  
Granule Neuron ◽  
Granule Neuron Precursors

ABSTRACT The forkhead transcription factor FoxM1 has been reported to regulate, variously, proliferation and/or spindle formation during the G2/M transition of the cell cycle. Here we define specific functions of FoxM1 during brain development by the investigation of FoxM1 loss-of-function mutations in the context of Sonic hedgehog (Shh)-induced neuroproliferation in cerebellar granule neuron precursors (CGNP). We show that FoxM1 is expressed in the cerebellar anlagen as well as in postnatal proliferating CGNP and that it is upregulated in response to activated Shh signaling. To determine the requirements for FoxM1 function, we used transgenic mice carrying conventional null alleles or conditionally targeted alleles in conjunction with specific Cre recombinase expression in CGNP or early neural precursors driven by Math1 or Nestin enhancers. Although the overall cerebellar morphology was grossly normal, we observed that the entry into mitosis was postponed both in vivo and in Shh-treated CGNP cultures. Cell cycle analysis and immunohistochemistry with antibodies against phosphorylated histone H3 indicated a significant delay in the G2/M transition. Consistent with this, FoxM1-deficient CGNP showed decreased levels of the cyclin B1 and Cdc25b proteins. Furthermore, the loss of FoxM1 resulted in spindle defects and centrosome amplification. These findings indicate that the functions of FoxM1 in Shh-induced neuroproliferation are restricted to the regulation of the G2/M transition in CGNP, most probably through transcriptional effects on target genes such as those coding for B-type cyclins.

scholarly journals Conditional Induction of Math1 Specifies Embryonic Stem Cells to Cerebellar Granule Neuron Lineage and Promotes Differentiation into Mature Granule Neurons

Stem Cells ◽  
2013 ◽  
Vol 31 (4) ◽  
pp. 652-665 ◽  
Author(s):  
Rupali Srivastava ◽  
Manoj Kumar ◽  
Stéphane Peineau ◽  
Zsolt Csaba ◽  
Shyamala Mani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Granule Neurons ◽  
Cerebellar Granule Neuron ◽  
Cerebellar Granule ◽  
Granule Neuron

scholarly journals ZNHIT3 is defective in PEHO syndrome, a severe encephalopathy with cerebellar granule neuron loss

Brain ◽  
2017 ◽  
Vol 140 (5) ◽  
pp. 1267-1279 ◽  
Author(s):  
Anna-Kaisa Anttonen ◽  
Anni Laari ◽  
Maria Kousi ◽  
Yawei J. Yang ◽  
Tiina Jääskeläinen ◽  
...  
Keyword(s):  
Cerebellar Granule Neuron ◽  
Neuron Loss ◽  
Cerebellar Granule ◽  
Granule Neuron
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