A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

10.3791/63312 ◽  
2021 ◽  
Author(s):  
Qingliang Yang ◽  
Jordan C. Langston ◽  
Yuan Tang ◽  
Balabhaskar Prabhakarpandian ◽  
Laurie E. Kilpatrick ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Interaction ◽  
Endothelial Cell Interaction

In Vivo Assessment of Synovial Microcirculation and Leukocyte–Endothelial Cell Interaction in Mouse Antigen-Induced Arthritis

1999 ◽  
Vol 6 (4) ◽  
pp. 281-290 ◽  
Author(s):  
A N D R E A S VEIHELMANN ◽  
ANTHONY G U S T A V E HARRIS ◽  
F R I T Z KROMBACH ◽  
E L K E SCHÜTZE ◽  
HANS JÜRGEN REFIOR ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Interaction ◽  
Endothelial Cell Interaction ◽  
In Vivo Assessment

A New Model for Quantitative In Vivo Microscopic Analysis of Thrombus Formation and Vascular Recanalisation: The Ear of the Hairless (hr/hr) Mouse

1997 ◽  
Vol 78 (05) ◽  
pp. 1408-1414 ◽  
Author(s):  
Frank Roesken ◽  
Martin Ruecker ◽  
Brigitte Vollmar ◽  
Nicole Boeckel ◽  
Eberhard Morgenstern ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Light Exposure ◽  
Thrombus Formation ◽  
Microscopic Analysis ◽  
Cell Interaction ◽  
Vascular Perfusion ◽  
Vessel Occlusion ◽  
Endothelial Cell Interaction ◽  
Platelet Deposition

SummaryThe alteration of rheological blood properties as well as deterioration of vascular perfusion conditions and cell-cell interactions are major determinants of thrombus formation. Herein, we present an experimental model which allows for quantitative in vivo microscopic analysis of these determinants during both thrombus formation and vascular recanalisation. The model does not require surgical preparation procedures, and enables for repeated analysis of identical microvessels over time periods of days or months, respectively. After i.v. administration of FITC-dextran thrombus formation was induced photochemically by light exposure to individual arterioles and venules of the ear of ten anaesthetised hairless mice. In venules, epiillumination induced rapid thrombus formation with first platelet deposition after 0.59 ± 0.04 min and complete vessel occlusion within 7.48 ±1.31 min. After a 24-h time period, 75% of the thrombosed venules were found recanalised. Marked leukocyte-endothelial cell interaction in those venules indicated persistent endothelial cell activation and/or injury, even after an observation period of 7 days. In arterioles, epi-illumination provoked vasomotion, while thrombus formation was significantly (p <0.05) delayed with first platelet deposition after 2.32 ± 0.22 min and complete vessel occlusion within 20.07 ±3.84 min. Strikingly, only one of the investigated arterioles was found recanalised after 24 h, which, however, did not show leukocyte-endothelial cell interaction. Heparin (300 U/kg, i.v.) effectively counteracted the process of thrombus formation in this model, including both first platelet deposition and vessel occlusion. We conclude that the model of the ear of the hairless mouse allows for distinct in vivo analysis of arteriolar and venular thrombus formation/ recanalisation, and, thus, represents an interesting tool for the study of novel antithrombotic and thrombolytic strategies, respectively.

Integrated Microfluidic Platform with Multiple Functions To Probe Tumor–Endothelial Cell Interaction

2017 ◽  
Vol 89 (18) ◽  
pp. 10037-10044 ◽  
Author(s):  
Ling Lin ◽  
Xuexia Lin ◽  
Luyao Lin ◽  
Qiang Feng ◽  
Takehiko Kitamori ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Interaction ◽  
Microfluidic Platform ◽  
Multiple Functions ◽  
Endothelial Cell Interaction ◽  
Tumor Endothelial Cell

Tumor-endothelial cell interaction in an experimental model of human hepatocellular carcinoma

2018 ◽  
Vol 372 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Azemat Jamshidi-Parsian ◽  
Robert J. Griffin ◽  
Rajshekhar A. Kore ◽  
Valentina K. Todorova ◽  
Issam Makhoul
Keyword(s):  
Hepatocellular Carcinoma ◽  
Endothelial Cell ◽  
Experimental Model ◽  
Cell Interaction ◽  
Human Hepatocellular Carcinoma ◽  
Endothelial Cell Interaction ◽  
Tumor Endothelial Cell

In VivoAssessment of Synovial Microcirculation and Leukocyte–Endothelial Cell Interaction in Mouse Antigen-Induced Arthritis

1999 ◽  
Vol 6 (4) ◽  
pp. 281-290 ◽  
Author(s):  
ANDREAS VEIHELMANN ◽  
ANTHONY GUSTAVE HARRIS ◽  
FRITZ KROMBACH ◽  
ELKE SCHÜTZE ◽  
HANS JÜRGEN REFIOR ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Interaction ◽  
Endothelial Cell Interaction

Basement Membrane Assembly and Differentiation of Cultured Corneal Cells: Importance of Culture Environment and Endothelial Cell Interaction

1994 ◽  
Vol 214 (2) ◽  
pp. 621-633 ◽  
Author(s):  
James D. Zieske ◽  
Valerie S. Mason ◽  
Michael E. Wasson ◽  
Susan F. Meunier ◽  
Cynthia J.M. Nolte ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Basement Membrane ◽  
Cell Interaction ◽  
Membrane Assembly ◽  
Endothelial Cell Interaction ◽  
Culture Environment ◽  
Corneal Cells
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