Organ Culture System for Assessing the Toxicity of Intraocular Treatment Excipients and Pharmaceuticals

10.3791/63176 ◽  
2022 ◽  
Author(s):  
Jordan Rossy ◽  
David J. McCanna ◽  
Bernard Fresco ◽  
Jacob G. Sivak
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

scholarly journals An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes

2013 ◽  
Vol 15 (5) ◽  
pp. R121 ◽  
Author(s):  
Dessislava Z Markova ◽  
Christopher K Kepler ◽  
Sankar Addya ◽  
Hallie B Murray ◽  
Alexander R Vaccaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Global Gene Expression ◽  
Degenerative Changes ◽  
Organ Culture System

scholarly journals Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential

2011 ◽  
Vol 20 (8) ◽  
pp. 1244-1254 ◽  
Author(s):  
Bernice Jim ◽  
Thomas Steffen ◽  
Janet Moir ◽  
Peter Roughley ◽  
Lisbet Haglund
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

scholarly journals In the absence of a basal lamina, ameloblasts absorb enamel in a serumless and chemically defined organ culture system

2021 ◽  
Author(s):  
Retsu Ohki ◽  
M. Matsuki-Fukushima ◽  
K. Fujikawa ◽  
Mitsuori Mayahara ◽  
Kayo Matsuyama ◽  
...  
Keyword(s):  
Organ Culture ◽  
Basal Lamina ◽  
Culture System ◽  
Organ Culture System

Bench-Top Validation Tests for Tissue-Engineered Arteries

2000 ◽  
Author(s):  
Shawn Chin Quee ◽  
Hai-Chao Han ◽  
David N. Ku
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Living System ◽  
Organ Culture System ◽  
Flow Pressure ◽  
Validation Tests

Abstract Standard tests are needed for evaluating and comparing the mechanical and biological functions of tissue engineered arteries and other vascular grafts. We propose an ex vivo organ culture system as a living system for testing tissue-engineered vascular grafts. This bench-top organ culture system mimics the physiological environment of arteries including the flow, pressure, and the axial stretch. Arterial mechanical properties and physiologic functions including compliance, burst pressure, and contractile functions can be assessed before an expensive long-term animal test is initiated. Test results of natural arteries indicate that organ culture is a valid model for comprehensive evaluation of tissue-engineered vascular grafts.

Axial Stretch Increases Cell Proliferation in Arteries in Organ Culture

2000 ◽  
Author(s):  
Hai-Chao Han ◽  
Raymond P. Vito ◽  
Kristin Michael ◽  
David N. Ku
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Organ Culture ◽  
Vascular Function ◽  
Culture System ◽  
Carotid Arteries ◽  
Ex Vivo ◽  
Axial Stretch ◽  
Organ Culture System ◽  
Physiological Flow

Abstract To study the effect of axial stretch on vascular function and wall remodeling, porcine carotid arteries were cultured under conditions of physiological flow and elevated axial stretch in an ex vivo organ culture system. Smooth muscle cell proliferation was measured by bromodeoxyuridine index. Results showed that cell proliferation was significantly increased in the highly stretched arteries when compared to the normally stretched arteries. This may indicate the feasibility of stimulating new arterial growth by stretching natural arteries.

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