Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

10.3791/63167 ◽  
2021 ◽  
Author(s):  
Weijia Lu ◽  
Ruimin Miao ◽  
Sijun Hu ◽  
Junhong Liu ◽  
Fanqi Jin ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Blood Sampling ◽  
Serial Blood ◽  
Vein Cannulation ◽  
Serial Blood Sampling

Simple Cannulation Procedure for Serial Blood Sampling Through Cutaneous Ulnar Vein in Chickens

2012 ◽  
Vol 15 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Darmel M. Bayer ◽  
K. Mohan ◽  
K. Jayakumar ◽  
Milad Manafi ◽  
B. H. Pavithra
Keyword(s):  
Blood Sampling ◽  
Serial Blood ◽  
Serial Blood Sampling

A method for catheterization, harnessing and chronic infusion of undisturbed chickens

1989 ◽  
Vol 23 (3) ◽  
pp. 270-274 ◽  
Author(s):  
T. L. Cravener ◽  
R. Vasilatos-Younken
Keyword(s):  
Blood Sampling ◽  
Free Movement ◽  
Indwelling Catheter ◽  
Serial Blood ◽  
Chronic Infusion ◽  
Serial Blood Sampling ◽  
Meat Type

A system was designed to facilitate the chronic infusion and/or serial blood sampling of rapidly growing, meat-type chickens with little or no stress to the bird. Techniques for surgically preparing an indwelling catheter, fabricating a harness system to protect this catheter, utilizing a miniature fluid swivel to provide free movement and infusing solutions via a programmable pump are described.

scholarly journals Usefulness of Serial Blood Sampling and PCR Replicates for Treatment Monitoring of Patients with Chronic Chagas Disease

2018 ◽  
Vol 63 (2) ◽  
pp. e01191-18 ◽  
Author(s):  
Rudy Parrado ◽  
Juan Carlos Ramirez ◽  
Anabelle de la Barra ◽  
Cristina Alonso-Vega ◽  
Natalia Juiz ◽  
...  
Keyword(s):  
Treatment Failure ◽  
Chagas Disease ◽  
Sampling Strategy ◽  
Blood Sampling ◽  
Clinical Sensitivity ◽  
Serial Blood ◽  
The Third ◽  
Two Samples ◽  
Serial Blood Sampling ◽  
Chronic Chagas Disease

ABSTRACT This work evaluated a serial blood sampling procedure to enhance the sensitivity of duplex real-time quantitative PCR (qPCR) for baseline detection and quantification of parasitic loads and posttreatment identification of failure in the context of clinical trials for treatment of chronic Chagas disease, namely, DNDi-CH-E1224-001 (ClinicalTrials.gov registration no. NCT01489228) and the MSF-DNDi PCR Sampling Optimization Study (NCT01678599). Patients from Cochabamba (n = 294), Tarija (n = 257), and Aiquile (n = 220) were enrolled. Three serial blood samples were collected at each time point, and qPCR triplicates were tested for each sample. The first two samples were collected during the same day and the third one 7 days later. A patient was considered PCR positive if at least one qPCR replicate was detectable. Cumulative results of multiple samples and qPCR replicates enhanced the proportion of pretreatment sample positivity from 54.8% to 76.2%, 59.5% to 77.8%, and 73.5% to 90.2% in Cochabamba, Tarija, and Aiquile cohorts, respectively. This strategy increased the detection of treatment failure from 72.9% to 91.7%, 77.8% to 88.9%, and 42.9% to 69.1% for E1224 low-, short-, and high-dosage regimens, respectively, and from 4.6% to 15.9% and 9.5% to 32.1% for the benznidazole arm in the DNDi-CH-E1224-001 and MSF-DNDi studies, respectively. The addition of the third blood sample and third qPCR replicate in patients with nondetectable PCR results in the first two samples gave a small, non-statistically significant improvement in qPCR positivity. No change in clinical sensitivity was seen with a blood volume increase from 5 to 10 ml. The monitoring of patients treated with placebo in the DNDi-CH-E1224-001 trial revealed fluctuations in parasitic loads and occasionally nondetectable results. In conclusion, a serial sampling strategy enhanced PCR sensitivity to detecting treatment failure during follow-up and has the potential for improving recruitment capacity in Chagas disease trials, which require an initial positive qPCR result for patient admission.

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