scholarly journals Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

10.3791/63049 ◽  
2021 ◽  
Author(s):  
Rashmi Thapa ◽  
Bo Liang ◽  
Rongsong Liu ◽  
Yun Li
Keyword(s):  
Calcium Imaging ◽  
Gradient Index ◽  
Lens Implantation ◽  
Gradient Index Lens ◽  
Deep Brain

scholarly journals In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems

2012 ◽  
Vol 3 (5) ◽  
pp. 1077 ◽  
Author(s):  
David M. Huland ◽  
Christopher M. Brown ◽  
Scott S. Howard ◽  
Dimitre G. Ouzounov ◽  
Ina Pavlova ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Gradient Index ◽  
Gradient Index Lens

scholarly journals A diencephalic pathway for movement initiation and rescue of Parkinsonian symptoms

2018 ◽  
Author(s):  
Glenn D.R. Watson ◽  
Ryan N. Hughes ◽  
Elijah A. Petter ◽  
Henry H. Yin
Keyword(s):  
Calcium Imaging ◽  
Movement Initiation ◽  
Movement Velocity ◽  
Parafascicular Nucleus ◽  
Behavioral Classification ◽  
Highly Correlated ◽  
3D Motion Capture ◽  
Major Target ◽  
Deep Brain

SummaryThe parafascicular nucleus (Pf) of the thalamus projects to the subthalamic nucleus (STN), a major target for deep brain stimulation (DBS) in Parkinson’s disease (PD), but the function of this projection remains unknown. Here, we used optogenetics, 3D motion capture, in vivo electrophysiology and 1-photon calcium imaging, unsupervised behavioral classification, and viral-based neuroanatomical tracing to examine the contribution of Pf efferents to movement generation in mice. We discovered that Pf neurons are highly correlated with movement velocity and excitation of Pf neurons generates turning and orienting movements. Movement initiation was not due to Pf projections to the striatum, but rather its projections to the STN. Optogenetic excitation of the Pf-STN pathway restores movement in a common mouse model of PD with complete akinesia. Collectively, our results reveal a thalamo-subthalamic pathway regulating movement initiation, and demonstrate a circuit mechanism that could potentially explain the clinical efficacy of DBS for relief of PD motor symptoms.

scholarly journals A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

2021 ◽  
Vol 15 ◽  
Author(s):  
Xiaoting Wu ◽  
Xiangyu Yang ◽  
Lulu Song ◽  
Yang Wang ◽  
Yamin Li ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Adult Mouse ◽  
Field Potential ◽  
Simultaneous Recording ◽  
Ca1 Region ◽  
Volume Weight ◽  
The Brain ◽  
Local Field Potential Lfp ◽  
Deep Brain

The miniscope system is one of the calcium (Ca2+) imaging tools with small size and lightweight and can realize the deep-brain Ca2+ imaging not confined to the cerebral cortex. Combining Ca2+ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatial resolution signals in the brain. In this study, a particular electrode probe was developed and assembled on the imaging lens to modify the miniscope system. The electrode probe can be tightly integrated into the lens of the miniscope without increasing the volume, weight, and implantation complexity. In vivo tests verified that the proposed modified system has realized the simultaneous recording of Ca2+ signals and local field potential (LFP) signal in the hippocampus CA1 region of an adult mouse.

Sonic Gradient Index Lens in Water

2010 ◽  
Author(s):  
Theodore P. Martin ◽  
Michael Nicholas ◽  
Gregory J. Orris ◽  
Liang-Wu Cai ◽  
Daniel Torrent ◽  
...  
Keyword(s):  
Gradient Index ◽  
Gradient Index Lens

Three-Dimensional Spatial Image Forming Using Gradient-Index Lens Arrays.

2002 ◽  
Vol 30 (12) ◽  
pp. 731-736
Author(s):  
Fumio OKANO ◽  
Jun ARAI ◽  
Makoto OKUI
Keyword(s):  
Three Dimensional ◽  
Gradient Index ◽  
Spatial Image ◽  
Gradient Index Lens

In Vivo Deep-Brain Structural and Hemodynamic Multiphoton Microscopy Enabled by Quantum Dots

Nano Letters ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 5260-5265 ◽  
Author(s):  
Hongji Liu ◽  
Xiangquan Deng ◽  
Shen Tong ◽  
Chen He ◽  
Hui Cheng ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Multiphoton Microscopy ◽  
Deep Brain

scholarly journals Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens

2009 ◽  
Vol 34 (11) ◽  
pp. 1684 ◽  
Author(s):  
Nicolas Olivier ◽  
Alexandre Mermillod-Blondin ◽  
Craig B. Arnold ◽  
Emmanuel Beaurepaire
Keyword(s):  
Depth Of Field ◽  
Gradient Index ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Gradient Index Lens ◽  
Extended Depth Of Field

scholarly journals Functional Microarchitecture of the Mouse Dorsal Inferior Colliculus Revealed through In Vivo Two-Photon Calcium Imaging

2015 ◽  
Vol 35 (31) ◽  
pp. 10927-10939 ◽  
Author(s):  
O. Barnstedt ◽  
P. Keating ◽  
Y. Weissenberger ◽  
A. J. King ◽  
J. C. Dahmen
Keyword(s):  
Inferior Colliculus ◽  
Calcium Imaging ◽  
Two Photon

Fiber compatible fast acousto‐optic modulator using a gradient index lens as the interaction medium

1993 ◽  
Vol 62 (26) ◽  
pp. 3402-3404 ◽  
Author(s):  
I. Abdulhalim ◽  
C. N. Pannell ◽  
D. N. Payne
Keyword(s):  
Gradient Index ◽  
Acousto Optic Modulator ◽  
Gradient Index Lens
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