Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

10.3791/62978 ◽  
2021 ◽  
Author(s):  
Zhiwei Xie ◽  
Jizhou Shi ◽  
Min Zong ◽  
Qiuping Xu ◽  
Chang Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Gingival Epithelial Cells ◽  
Human Gingival Epithelial Cells

scholarly journals Cigarette Smoke Stimulates SARS-CoV-2 Internalization by Activating AhR and Increasing ACE2 Expression in Human Gingival Epithelial Cells

2021 ◽  
Vol 22 (14) ◽  
pp. 7669
Author(s):  
Cassio Luiz Coutinho Almeida-da-Silva ◽  
Harmony Matshik Dakafay ◽  
Kaitlyn Liu ◽  
David M. Ojcius
Keyword(s):  
Epithelial Cells ◽  
Oral Cavity ◽  
Cigarette Smoke ◽  
Large Body ◽  
Receptor Expression ◽  
Host Cells ◽  
Dependent Manner ◽  
Gingival Epithelial Cells ◽  
Human Gingival Epithelial Cells ◽  
Oral Cells

A large body of evidence shows the harmful effects of cigarette smoke to oral and systemic health. More recently, a link between smoking and susceptibility to coronavirus disease 2019 (COVID-19) was proposed. COVID-19 is due to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which uses the receptor ACE2 and the protease TMPRSS2 for entry into host cells, thereby infecting cells of the respiratory tract and the oral cavity. Here, we examined the effects of cigarette smoke on the expression of SARS-CoV-2 receptors and infection in human gingival epithelial cells (GECs). We found that cigarette smoke condensates (CSC) upregulated ACE2 and TMPRSS2 expression in GECs, and that CSC activated aryl hydrocarbon receptor (AhR) signaling in the oral cells. ACE2 was known to mediate SARS-CoV-2 internalization, and we demonstrate that CSC treatment potentiated the internalization of SARS-CoV-2 pseudovirus in GECs in an AhR-dependent manner. AhR depletion using small interference RNA decreased SARS-CoV-2 pseudovirus internalization in CSC-treated GECs compared with control GECs. Our study reveals that cigarette smoke upregulates SARS-CoV-2 receptor expression and infection in oral cells. Understanding the mechanisms involved in SARS-CoV-2 infection in cells of the oral cavity may suggest therapeutic interventions for preventing viral infection and transmission.

scholarly journals Identification of Porphyromonas gingivalis Genes Specifically Expressed in Human Gingival Epithelial Cells by Using Differential Display Reverse Transcription-PCR

2004 ◽  
Vol 72 (7) ◽  
pp. 3752-3758 ◽  
Author(s):  
Yoonsuk Park ◽  
Özlem Yilmaz ◽  
Il-Young Jung ◽  
Richard J. Lamont
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Abc Transporter ◽  
Differential Display ◽  
Molecular Mechanisms ◽  
Gene Products ◽  
Reverse Transcription Pcr ◽  
Gingival Epithelial Cells ◽  
Insertional Inactivation ◽  
Human Gingival Epithelial Cells

ABSTRACT Porphyromonas gingivalis, one of the causative agents of adult periodontitis, can invade and survive within host epithelial cells. The molecular mechanisms by which P. gingivalis induces uptake and adapts to an intracellular environment are not fully understood. In this study, we have investigated the genetic responses of P. gingivalis internalized within human gingival epithelial cells (GECs) in order to identify factors involved in invasion and survival. We compared the differential display of arbitrarily PCR-amplified gene transcripts in P. gingivalis recovered from GECs with the display of transcripts in P. gingivalis control cultures. Over 20 potential differentially expressed transcripts were identified. Among these, pepO, encoding an endopeptidase, and genes encoding an ATP-binding cassette (ABC) transporter and a cation-transporting ATPase were upregulated in GECs. To investigate the functionality of these gene products, mutants were generated by insertional inactivation. Compared to the parental strain, mutants of each gene showed a significant reduction in their invasion capabilities. In addition, GEC cytoskeletal responses to the mutants were distinct from those induced by the parent. In contrast, adhesion of the mutant strains to GECs was not affected by lack of expression of the gene products. These results suggest that PepO, a cation-transporting ATPase, and an ABC transporter are required for the intracellular lifestyle of P. gingivalis.

scholarly journals Response of Human Gingival Epithelial Cells (HGE-15) to Bioactive Glass RKKP

2008 ◽  
Vol 7 (1) ◽  
pp. 5-11
Author(s):  
Hitoshi Oguchi ◽  
Yasuyo Karube ◽  
Kameji Matsumoto ◽  
Mitsuhiko Morito
Keyword(s):  
Epithelial Cells ◽  
Bioactive Glass ◽  
Gingival Epithelial Cells ◽  
Human Gingival Epithelial Cells

scholarly journals TNF-α Expression in Oral Candida albicans-Infected Human Gingival Epithelial Cells

2011 ◽  
Vol 10 (2) ◽  
pp. 77-82 ◽  
Author(s):  
Yoko Tanaka ◽  
Lin Zhang ◽  
Tonami Ikuta ◽  
Joutaro Omori ◽  
Hirotaka Omine ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Gingival Epithelial Cells ◽  
Tnf Α ◽  
Oral Candida ◽  
Human Gingival Epithelial Cells

Lipoproteins of Actinomyces viscosus induce inflammatory responses through TLR2 in human gingival epithelial cells and macrophages

2012 ◽  
Vol 14 (11) ◽  
pp. 916-921 ◽  
Author(s):  
Eri Shimada ◽  
Hideo Kataoka ◽  
Yasushi Miyazawa ◽  
Matsuo Yamamoto ◽  
Takeshi Igarashi
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Responses ◽  
Gingival Epithelial Cells ◽  
Human Gingival Epithelial Cells

IFN-β secretion is through TLR3 but not TLR4 in human gingival epithelial cells

2019 ◽  
Vol 111 ◽  
pp. 27-31 ◽  
Author(s):  
Hellen Teixeira ◽  
Jiawei Zhao ◽  
Denis F. Kinane ◽  
Manjunatha R. Benakanakere
Keyword(s):  
Epithelial Cells ◽  
Gingival Epithelial Cells ◽  
Human Gingival Epithelial Cells
Sign in / Sign up

Export Citation Format

Share Document