scholarly journals Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

10.3791/62954 ◽  
2021 ◽  
Author(s):  
Katherina Hemmen ◽  
Susobhan Choudhury ◽  
Mike Friedrich ◽  
Johannes Balkenhol ◽  
Felix Knote ◽  
...  
Keyword(s):  
Protein Dynamics ◽  
Protein Interaction ◽  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Live Cells ◽  
Protein Protein Interaction ◽  
Dual Color

scholarly journals Protein–protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy

2006 ◽  
Vol 34 (14) ◽  
pp. e102-e102 ◽  
Author(s):  
Rieko Oyama ◽  
Hideaki Takashima ◽  
Masato Yonezawa ◽  
Nobuhide Doi ◽  
Etsuko Miyamoto-Sato ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Cross Correlation ◽  
Interaction Analysis ◽  
Correlation Spectroscopy ◽  
Fluorescence Labeling ◽  
Specific Fluorescence ◽  
Protein Protein Interaction

Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

Lab on a Chip ◽  
2011 ◽  
Vol 11 (8) ◽  
pp. 1502 ◽  
Author(s):  
A. Chen ◽  
M. M. Eberle ◽  
E. J. Lunt ◽  
S. Liu ◽  
K. Leake ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Dual Color ◽  
Optofluidic Chip

scholarly journals Fluorescence Correlation and Cross-Correlation Spectroscopy Unveil Cytoplasmic mRNP Composition and Dynamics

2020 ◽  
Author(s):  
Àngels Mateu-Regué ◽  
Jan Christiansen ◽  
Christian Hellriegel ◽  
Finn Cilius Nielsen
Keyword(s):  
Life Cycle ◽  
Dynamic Analysis ◽  
Cross Correlation ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Correlation Spectroscopy ◽  
Live Cells ◽  
Macromolecular Complexes ◽  
Fluorescence Correlation ◽  
Macromolecular Composition

ABSTRACTUnderstanding the mRNA life cycle requires analysis of the dynamic macromolecular composition and stoichiometry of mRNPs. Fluorescence correlation and cross-correlation spectroscopy (FCS and FCCS) are appealing technologies to study mRNP complexes because they readily provide information about the molecular composition, stoichiometry, heterogeneity and dynamics of the particles. We developed FCS protocols for analysis of live cells and cellular lysates, and demonstrate the feasibility of analysing common cytoplasmic mRNPs composed of core factor YBX1, IMPs (or IGF2BPs) and their interactions with other RNA binding proteins such as PABPC1, ELAVL2 (HuB), STAU1 and FMRP. FCCS corroborated previously reported RNA dependent interactions between the factors and provided an estimate of the relative overlap between the factors in the mRNPs. In this way FCS and FCCS provide a new and useful approach for the quantitative and dynamic analysis of mRNP macromolecular complexes that may complement current biochemical approaches.

A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy

2006 ◽  
Vol 24 (5) ◽  
pp. 577-581 ◽  
Author(s):  
Takako Kogure ◽  
Satoshi Karasawa ◽  
Toshio Araki ◽  
Kenta Saito ◽  
Masataka Kinjo ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Laser Fluorescence ◽  
Stony Coral ◽  
Single Laser ◽  
Dual Color

Dual-color Fluorescence Cross Correlation Spectroscopy on an Integrated Optofluidic Chip

2010 ◽  
Author(s):  
A. Chen ◽  
M.I. Rudenko ◽  
E.J. Lunt ◽  
B.S. Phillips ◽  
A. R. Hawkins ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Dual Color ◽  
Optofluidic Chip
Sign in / Sign up

Export Citation Format

Share Document