Generation and Characterization of Murine Oral Mucosal Organoid Cultures

10.3791/62529 ◽  
2021 ◽  
Author(s):  
Anna C. Seubert ◽  
Marion Krafft ◽  
Kai Kretzschmar
Keyword(s):  
Organoid Cultures

scholarly journals Development and characterization of salivary gland cancer organoid cultures

2019 ◽  
Vol 30 ◽  
pp. v472-v473
Author(s):  
W.V. Boxtel ◽  
T.M.W. Aalders ◽  
G. Lassche ◽  
I.C.H. van Engen - van Grunsven ◽  
G.W. Verhaegh ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Salivary Gland Cancer ◽  
Organoid Cultures

Characterization of hepatic-organoid cultures

2009 ◽  
Vol 42 (3) ◽  
pp. 472-481 ◽  
Author(s):  
Toshihiro Mitaka ◽  
Hidekazu Ooe
Keyword(s):  
Organoid Cultures

scholarly journals Characterization of Organoid Cultures to Study the Effects of Pregnancy Hormones on the Epigenome and Transcriptional Output of Mammary Epithelial Cells

2020 ◽  
Author(s):  
Michael F. Ciccone ◽  
Marygrace C. Trousdell ◽  
Camila O. dos Santos
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Ex Vivo ◽  
Mammary Epithelial ◽  
Suitable Model ◽  
Activation Of Transcription ◽  
Organoid Cultures ◽  
Pregnancy Hormones

Abstract The use of mouse derived mammary organoids can provide a unique strategy to study mammary gland development across a normal life cycle, as well as offering insights into how malignancies form and progress. Substantial cellular and epigenomic changes are triggered in response to pregnancy hormones, a reaction that engages molecular and cellular changes that transform the mammary epithelial cells into “milk producing machines”. Such epigenomic alterations remain stable in post-involution mammary epithelial cells and control the reactivation of gene transcription in response to re-exposure to pregnancy hormones. Thus, a system that tightly controls exposure to pregnancy hormones, epigenomic alterations, and activation of transcription will allow for a better understanding of such molecular switches. Here, we describe the characterization of ex vivo cultures to mimic the response of mammary organoid cultures to pregnancy hormones and to understand gene regulation and epigenomic reprogramming on consecutive hormone exposure. Our findings suggest that this system yields similar epigenetic modifications to those reported in vivo, thus representing a suitable model to closely track epigenomic rearrangement and define unknown players of pregnancy-induced development.

Characterization of bladder organoid cultures from healthy and cancer tissues

2019 ◽  
Vol 18 (1) ◽  
pp. e660
Author(s):  
P.M. Patard ◽  
A. Rubio ◽  
V. Tostivint ◽  
C. Rouget ◽  
P. Lluel ◽  
...  
Keyword(s):  
Organoid Cultures ◽  
Cancer Tissues

Characterization of bicarbonate secretion in human pancreatic ductal organoid cultures

Pancreatology ◽  
2021 ◽  
Vol 21 ◽  
pp. S2
Author(s):  
T. Madacsy ◽  
A. Varga ◽  
A. Kiss ◽  
V. Szabo ◽  
T. Takacs ◽  
...  
Keyword(s):  
Bicarbonate Secretion ◽  
Organoid Cultures

Characterization of canine epidermal organoid cultures by immunohistochemical analysis and quantitative PCR

2020 ◽  
Author(s):  
Dominique J. Wiener ◽  
Isabelle C. Studer ◽  
Magdalena A.T. Brunner ◽  
Andreas Hermann ◽  
Simona Vincenti ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Immunohistochemical Analysis ◽  
Organoid Cultures

Characterization of HCO3- secretory processes in human pancreatic ductal organoid cultures

Pancreatology ◽  
2020 ◽  
Vol 20 ◽  
pp. S83-S84
Author(s):  
T. Madacsy ◽  
A. Varga ◽  
A. Kiss ◽  
V. Szabo ◽  
T. Takacs ◽  
...  
Keyword(s):  
Organoid Cultures

Characterization of 2D and 3D organoid cultures derived from mouse and human pancreas

Pancreatology ◽  
2020 ◽  
Vol 20 ◽  
pp. S82
Author(s):  
V. Szabó ◽  
Varga î ◽  
J. Maléth
Keyword(s):  
Human Pancreas ◽  
Organoid Cultures ◽  
2D And 3D

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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