Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation

10.3791/62499 ◽  
2021 ◽  
Author(s):  
Anaïs Pongerard ◽  
Lea Mallo ◽  
Christian Gachet ◽  
Henri de La Salle ◽  
François Lanza ◽  
...  
Keyword(s):  
Cd34 Cells ◽  
Cell Source ◽  
A Cell ◽  
Megakaryocyte Differentiation ◽  
Platelet Formation

Thromboxane Synthase Has the Same Pattern of Expression as Platelet Specific Glycoproteins during Human Megakaryocyte Differentiation

2000 ◽  
Vol 83 (05) ◽  
pp. 759-768 ◽  
Author(s):  
Natacha Vitrat ◽  
Rémi Letestu ◽  
Aline Massé ◽  
Vladimir Lazar ◽  
Najet Debili ◽  
...  
Keyword(s):  
Formation Process ◽  
The Other ◽  
Rt Pcr ◽  
Thromboxane Synthase ◽  
Platelet Production ◽  
Cd34 Cells ◽  
Specific Proteins ◽  
Deficient Mice ◽  
Megakaryocyte Differentiation ◽  
Platelet Formation

SummaryRegulation of the platelet formation process is poorly understood. It has been shown that p45NF-E2 deficient mice have a profound defect in platelet formation and recently the first platelet/megakaryocytic gene regulated by NF-E2, thromboxane synthase (TXS), has been identified. In this study, we investigated TXS expression as a model of a gene regulated by NF-E2 during MK differentiation. Megakaryocytic cells derived from blood CD34+ cells were purified according to their stage of maturation on the basis of expression of CD34, CD41a and CD42a, permitting to define different stages in MK differentiation. By means of real-time quantitative RT-PCR, we could determine that the level of TXS increased during differentiation in parallel with the expression of c-mpl and GPIIb (CD41). However, amounts of TXS transcripts increased about 1.6-fold more than that of GPIIb or c-mpl transcripts during maturation. Expression of TXS and MK specific proteins such as CD41a, CD42a and vWF was also correlated in maturing MKs. In addition, staining by anti-TXS antibody of proplatelet bearing MKs was not increased in comparison to that observed in mature MK, suggesting that TXS is not upregulated during platelet formation. In addition, we investigated whether TXS and cyclooxygenase could be involved in platelet formation by adding aspirin into the cultures. No significant decrease of platelet production was observed.In conclusion, this study shows that TXS is coordinately expressed with the other platelet proteins during MK differentiation but is not directly involved in platelet formation.

Cholangiocyte Organoids as a Cell Source for Biliary Repair

2021 ◽  
Author(s):  
Jeremy J Velazquez ◽  
Mo R Ebrahimkhani
Keyword(s):  
Cell Source ◽  
A Cell

scholarly journals Aging: A cell source limiting factor in tissue engineering

2019 ◽  
Vol 11 (10) ◽  
pp. 787-802 ◽  
Author(s):  
Mohammadhossein Khorraminejad-Shirazi ◽  
Mohammadreza Dorvash ◽  
Alireza Estedlal ◽  
Amir Human Hoveidaei ◽  
Mohsen Mazloomrezaei ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Limiting Factor ◽  
Cell Source ◽  
A Cell

Use of Embryonic Stem Cell-Derived Endothelial Cells as a Cell Source to Generate Vessel Structuresin Vitro

2005 ◽  
Vol 11 (3-4) ◽  
pp. 497-505 ◽  
Author(s):  
Kara E. McCloskey ◽  
Meghan E. Gilroy ◽  
Robert M. Nerem
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cell Source ◽  
A Cell

scholarly journals Arachidonic acid and Docosahexanoic acid enhance platelet formation from human apheresis-derived CD34+ cells

Cell Cycle ◽  
2017 ◽  
Vol 16 (10) ◽  
pp. 979-990 ◽  
Author(s):  
Ankita Dhenge ◽  
Kedar Limbkar ◽  
Sameer Melinkeri ◽  
Vaijayanti Prakash Kale ◽  
Lalita Limaye
Keyword(s):  
Arachidonic Acid ◽  
Docosahexanoic Acid ◽  
Cd34 Cells ◽  
Platelet Formation

Embryonic Stem Cells as a Cell Source for Tissue Engineering

2007 ◽  
pp. 445-458
Author(s):  
Ali Khademhosseini ◽  
Jeffrey M. Karp ◽  
Sharon Gerecht ◽  
Lino Ferreira ◽  
Gordana Vunjak-Novakovic ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Source ◽  
A Cell

379 Restoring urea cycle function in HepG2 cells by multiple gene transfer; A cell source for a bio-artificial liver device

2006 ◽  
Vol 44 ◽  
pp. S144 ◽  
Author(s):  
D. Mavd-Damelin ◽  
S. Eaton ◽  
S. Coward ◽  
L. Damelin ◽  
M. Rees ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Hepg2 Cells ◽  
Urea Cycle ◽  
Artificial Liver ◽  
Multiple Gene ◽  
Cell Source ◽  
A Cell
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