Isolation of Mouse Megakaryocyte Progenitors

10.3791/62498 ◽  
2021 ◽  
Author(s):  
Quentin Kimmerlin ◽  
Manuela Tavian ◽  
Christian Gachet ◽  
François Lanza ◽  
Nathalie Brouard
Keyword(s):  
Megakaryocyte Progenitors

scholarly journals Recombinant human interleukin-11 stimulates megakaryocytopoiesis and increases peripheral platelets in normal and splenectomized mice

Blood ◽  
1993 ◽  
Vol 81 (4) ◽  
pp. 901-908 ◽  
Author(s):  
TY Neben ◽  
J Loebelenz ◽  
L Hayes ◽  
K McCarthy ◽  
J Stoudemire ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Color Flow ◽  
Platelet Counts ◽  
In Vivo Treatment ◽  
Interleukin 11 ◽  
Megakaryocyte Progenitors

Abstract The effects of recombinant human interleukin-11 (rhIL-11) on in vivo mouse megakaryocytopoeisis were examined. Normal C57Bl/6 mice and splenectomized C57Bl/6 mice were treated for 7 days with 150 micrograms/kg rhIL-11 administered subcutaneously. In normal mice, peripheral platelet counts were elevated compared with vehicle-treated controls after 3 days of rhIL-11 treatment and remained elevated until day 10. Splenectomized mice treated with rhIL-11 showed elevated peripheral platelet counts that were similar in magnitude to normal rhIL-11-treated mice. However, on day 10 the platelet counts in rhIL-11- treated, splenectomized mice were no longer elevated. Analysis of bone marrow megakaryocyte ploidy by two-color flow cytometry showed an increase, relative to controls, in the percentage of 32N megakaryocytes in both normal and splenectomized animals treated with rhIL-11. In normal mice, the number of spleen megakaryocyte colony-forming cells (MEG-CFC) were increased twofold to threefold relative to controls after 3 and 7 days of rhIL-11 treatment, whereas the number of bone marrow MEG-CFC were increased only on day 7. The number of MEG-CFC in the bone marrow of rhIL-11-treated, splenectomized mice was increased twofold compared with controls on both days 3 and 7 of the study. These data show that in vivo treatment of normal or splenectomized mice with rhIL-11 increased megakaryocyte progenitors, stimulated endoreplication of bone marrow megakaryocytes, and increased peripheral platelet counts. In addition, results in splenectomized mice showed that splenic hematopoiesis was not essential for the observed increases in peripheral platelets in response to rhIL-11 administration.

scholarly journals Early megakaryocyte lineage-committed progenitors in adult mouse bone marrow

2021 ◽  
Author(s):  
Zixian Liu ◽  
Jinhong Wang ◽  
Miner Xie ◽  
Peng Wu ◽  
Yao Ma ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Single Cell ◽  
Pcr Analysis ◽  
Mouse Bone Marrow ◽  
Hematopoietic Stem ◽  
Colony Assay ◽  
Megakaryocyte Progenitors ◽  
Cell Colony

Hematopoietic stem cells (HSCs) have been considered to progressively lose their self-renewal and differentiation potentials prior to the commitment to each blood lineage. However, recent studies have suggested that megakaryocyte progenitors are generated at the level of HSCs. In this study, we newly identified early megakaryocyte lineage-committed progenitors (MgPs) in CD201-CD48- cells and CD48+ cells separated from the CD150+CD34-Kit+Sca-1+Lin- HSC population of the bone marrow in C57BL/6 mice. Single-cell transplantation and single-cell colony assay showed that MgPs, unlike platelet-biased HSCs, had little repopulating potential in vivo, but formed larger megakaryocyte colonies in vitro (on average eight megakaryocytes per colony) than did previously reported megakaryocyte progenitors (MkPs). Single-cell RNA-sequencing supported that these MgPs lie between HSCs and MkPs along the megakaryocyte differentiation pathway. Single-cell colony assay and single-cell RT-PCR analysis suggested the coexpression of CD41 and Pf4 is associated with megakaryocyte colony-forming activity. Single-cell colony assay of a small number of cells generated from single HSCs in culture suggested that MgPs are not direct progeny of HSCs. In this study, we propose a differentiation model in which HSCs give rise to MkPs through MgPs.

Deficiency of ATP binding cassette transporter b6 in megakaryocyte progenitors accelerates atherosclerosis in mice

2014 ◽  
Vol 235 (2) ◽  
pp. e28
Author(s):  
A. Murphy ◽  
V. Sarrazy ◽  
N. Wang ◽  
N. Bijl ◽  
S. Abramowicz ◽  
...  
Keyword(s):  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Megakaryocyte Progenitors ◽  
Atp Binding Cassette

scholarly journals Direct Conversion of Fibroblasts to Megakaryocyte Progenitors

Cell Reports ◽  
2016 ◽  
Vol 17 (3) ◽  
pp. 671-683 ◽  
Author(s):  
Julian Pulecio ◽  
Oriol Alejo-Valle ◽  
Sandra Capellera-Garcia ◽  
Marianna Vitaloni ◽  
Paula Rio ◽  
...  
Keyword(s):  
Direct Conversion ◽  
Megakaryocyte Progenitors

scholarly journals Serum‐free medium allows the optimal growth of human megakaryocyte progenitors compared with human plasma supplemented cultures: Role of TGF β

Stem Cells ◽  
1993 ◽  
Vol 11 (2) ◽  
pp. 120-129 ◽  
Author(s):  
Rolande Berthier ◽  
Odile Valiron ◽  
Annie Schweitzer ◽  
Gérard Marguerie
Keyword(s):  
Human Plasma ◽  
Optimal Growth ◽  
Tgf Beta ◽  
Free Medium ◽  
Megakaryocyte Progenitors

The response of cord blood megakaryocyte progenitors to IL-3, IL-6 and aplastic canine serum varies with gestational age

1995 ◽  
Vol 89 (1) ◽  
pp. 8-16 ◽  
Author(s):  
Varda R. Deutsch ◽  
Thomas A. Olson ◽  
Arnon Nagler ◽  
Shimon Slavin ◽  
Richard F. Levine ◽  
...  
Keyword(s):  
Cord Blood ◽  
Gestational Age ◽  
Canine Serum ◽  
Megakaryocyte Progenitors

scholarly journals Megakaryocyte Progenitors Are the Main APCs Inducing Th17 Response to Lupus Autoantigens and Foreign Antigens

2012 ◽  
Vol 188 (12) ◽  
pp. 5970-5980 ◽  
Author(s):  
Hee-Kap Kang ◽  
Ming-Yi Chiang ◽  
Diane Ecklund ◽  
Li Zhang ◽  
Rosalind Ramsey-Goldman ◽  
...  
Keyword(s):  
Th17 Response ◽  
Megakaryocyte Progenitors

INCREASED SPONTANEOUS NUMBER OF MEGAKARYOCYTE COLONIES IN ESSENTIAL THROMBOCYTHEMIA (ET)

1987 ◽  
Author(s):  
J F Deschamps ◽  
E Bodevin ◽  
J P Caen
Keyword(s):  
Platelet Count ◽  
Human Serum ◽  
Progenitor Cells ◽  
Platelet Function ◽  
Essential Thrombocythemia ◽  
Antiplatelet Agents ◽  
Iron Therapy ◽  
Plasma Clot ◽  
Megakaryocyte Progenitors

Megakaryocytes were grown from medullary progenitor cells using the plasma clot technique, with 5 % human serum and with or without 2,5 % PHA-LCM. Using this technique megakaryocyte cultures were done in 5 patients essential thrombocythemia (ET) ( platelet count :0,6 x 106 to 1,4 x 106 /μi) before chemotherapy and antiplatelet agents and in 2 patients with secondary thrombocytosis (ST) (platelet count : 0,65 x lO6 and 0,8 x 106 /μi) corrected after effective anti-bacterial or iron therapy. The results were as followsx number of colonies mean number (in brackets) of cells per colonyIt appears therefore that in ET, megakaryocyte progenitors grow without PHA-LCM and show however a better proliferation in its presence. On the contrary in ST, PHA-LCM is required for obtaining a 6 times increase of MK colonies. The effect of MK growth under chemotherapy is reported in some of the patients studied before treatment and results analyzed and compared to platelet function involvement

Ex VivoExpansion of Early and Late Megakaryocyte Progenitors

2000 ◽  
Vol 9 (6) ◽  
pp. 913-921 ◽  
Author(s):  
Phil Lefebvre ◽  
Jane N. Winter ◽  
Yuru Meng ◽  
Isaac Cohen
Keyword(s):  
Megakaryocyte Progenitors
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